Open Access

Polygonatum sibiricum polysaccharides prevent LPS‑induced acute lung injury by inhibiting inflammation via the TLR4/Myd88/NF‑κB pathway

  • Authors:
    • Tian‑Yin Liu
    • Li‑Li Zhao
    • Shi‑Biao Chen
    • Ben‑Chao Hou
    • Jian Huang
    • Xiu Hong
    • Lian Qing
    • Yu Fang
    • Zhe Tao
  • View Affiliations

  • Published online on: August 5, 2020     https://doi.org/10.3892/etm.2020.9097
  • Pages: 3733-3739
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Inflammation plays an important role in cases of acute lung injury (ALI), and the Toll‑like receptor 4/nuclear factor‑κB (TLR4/NF‑κB) pathway, which can be regulated by Polygonatum sibiricum polysaccharides (PSPs), is closely related to the dynamics of lipopolysaccharide (LPS)‑induced inflammation. Thus, we sought to evaluate whether or not PSPs prevent LPS‑induced ALI by way of inhibiting inflammation via the TLR4/NF‑κB pathway in rats. We established an ALI rat model by tracheal instillation of LPS, and by pre‑injection of PSPs into rats to examine PSPs in the ALI rat model. We found that PSPs attenuated LPS‑induced lung pathological changes in ALI rats, decreased LPS‑induced myeloperoxidase (MOP) activity, and elevated malondialdehyde (MDA) levels in lung tissue. However, PSPs also decreased the LPS‑induced increase in the neutrophil ratio, and decreased inflammatory factor levels in bronchoalveolar lavage fluid (BALF). Moreover, PSPs decreased LPS‑induced increases in inflammatory factors measured by mRNA expression, and altered the levels of expression of TLR4, medullary differentiation protein 88 (Myd88), p‑IKB‑α/IKB‑α and p‑p65/p65 proteins in lung tissue. In vitro, PSPs also reduced apoptosis induced by LPS in BEAS‑2B cells by suppressing inflammation through its effect of inhibiting the TLR4/NF‑κB pathway. In conclusion, our results suggest that PSPs may be a potential drug for effective treatment of LPS‑induced ALI, due to the ability to inhibit inflammation through effects exerted on the TLR4/Myd88/NF‑κB pathway.
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October-2020
Volume 20 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu TY, Zhao LL, Chen SB, Hou BC, Huang J, Hong X, Qing L, Fang Y and Tao Z: Polygonatum sibiricum polysaccharides prevent LPS‑induced acute lung injury by inhibiting inflammation via the TLR4/Myd88/NF‑κB pathway. Exp Ther Med 20: 3733-3739, 2020.
APA
Liu, T., Zhao, L., Chen, S., Hou, B., Huang, J., Hong, X. ... Tao, Z. (2020). Polygonatum sibiricum polysaccharides prevent LPS‑induced acute lung injury by inhibiting inflammation via the TLR4/Myd88/NF‑κB pathway. Experimental and Therapeutic Medicine, 20, 3733-3739. https://doi.org/10.3892/etm.2020.9097
MLA
Liu, T., Zhao, L., Chen, S., Hou, B., Huang, J., Hong, X., Qing, L., Fang, Y., Tao, Z."Polygonatum sibiricum polysaccharides prevent LPS‑induced acute lung injury by inhibiting inflammation via the TLR4/Myd88/NF‑κB pathway". Experimental and Therapeutic Medicine 20.4 (2020): 3733-3739.
Chicago
Liu, T., Zhao, L., Chen, S., Hou, B., Huang, J., Hong, X., Qing, L., Fang, Y., Tao, Z."Polygonatum sibiricum polysaccharides prevent LPS‑induced acute lung injury by inhibiting inflammation via the TLR4/Myd88/NF‑κB pathway". Experimental and Therapeutic Medicine 20, no. 4 (2020): 3733-3739. https://doi.org/10.3892/etm.2020.9097