Open Access

UBR5 oncogene as an indicator of poor prognosis in gastric cancer

  • Authors:
    • Fanghui Ding
    • Xiaoliang Zhu
    • Xiaojing Song
    • Pei Yuan
    • Longfei  Ren
    • Changpeng Chai
    • Wence Zhou
    • Xun Li
  • View Affiliations

  • Published online on: August 25, 2020     https://doi.org/10.3892/etm.2020.9135
  • Article Number: 7
  • Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The human ubiquitin protein ligase E3 component N‑recognin 5 (UBR5) gene, which is localized to chromosome 8q22, encodes an ~10 kb mRNA and a >300 kDa protein, which can be detected in a number of cell types. UBR5 is implicated in several types of cancer, including ovarian cancer, gallbladder cancer and lymphoma; however, its role in gastric cancer is not completely understood. In the present study, the expression levels of UBR5 in human gastric cancer tissues and cell lines were examined via immunohistochemistry, reverse transcription‑quantitative PCR analysis and western blotting. Furthermore, the association between UBR5 expression and clinicopathological characteristics, as well as the prognosis of patients with gastric cancer, were examined. In addition, the role of UBR5 in gastric cancer cell proliferation, invasion and migration was investigated by conducting MTS, Transwell and wound healing assays, respectively. The results indicated that the mRNA and protein expression levels of UBR5 were significantly increased in gastric cancer tissues compared with para‑carcinoma tissues. High UBR5 expression levels were significantly associated with larger tumor size, advanced TNM stage and lymph node metastasis. In addition, high UBR5 expression indicated a poor prognosis in patients with gastric cancer. Furthermore, in vitro experiments demonstrated that UBR5 knockdown was associated with reduced HGC‑27 gastric cancer cell proliferation, invasion and migration compared with the small interfering RNA control group. Therefore, the results indicated that UBR5 may serve a key role in gastric cancer, indicating that UBR5 may also serve as an important prognostic biomarker.
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November-2020
Volume 20 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Ding F, Zhu X, Song X, Yuan P, Ren L, Chai C, Zhou W and Li X: UBR5 oncogene as an indicator of poor prognosis in gastric cancer. Exp Ther Med 20: 7, 2020.
APA
Ding, F., Zhu, X., Song, X., Yuan, P., Ren, L., Chai, C. ... Li, X. (2020). UBR5 oncogene as an indicator of poor prognosis in gastric cancer. Experimental and Therapeutic Medicine, 20, 7. https://doi.org/10.3892/etm.2020.9135
MLA
Ding, F., Zhu, X., Song, X., Yuan, P., Ren, L., Chai, C., Zhou, W., Li, X."UBR5 oncogene as an indicator of poor prognosis in gastric cancer". Experimental and Therapeutic Medicine 20.5 (2020): 7.
Chicago
Ding, F., Zhu, X., Song, X., Yuan, P., Ren, L., Chai, C., Zhou, W., Li, X."UBR5 oncogene as an indicator of poor prognosis in gastric cancer". Experimental and Therapeutic Medicine 20, no. 5 (2020): 7. https://doi.org/10.3892/etm.2020.9135