Open Access

Combined culture experiment of mouse bone marrow mesenchymal stem cells and bioceramic scaffolds

  • Authors:
    • Xin Huang
    • Zhenhao Chen
    • Guanglei Zhao
    • Jingsheng Shi
    • Gangyong Huang
    • Feiyan Chen
    • Yibing Wei
    • Jun Xia
    • Jie Chen
    • Siqun Wang
  • View Affiliations

  • Published online on: August 27, 2020     https://doi.org/10.3892/etm.2020.9147
  • Article Number: 19
  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Articular cartilage injuries are common orthopedic conditions that severely affect the quality of life of patients. Tissue engineering can facilitate cartilage repair and the key points involve scaffolding and seed cell selection. Pre‑experiments found a range of microstructures of bioceramic scaffolds suitable for chondrocyte adhesion and proliferation, and maintaining chondrocyte phenotype. Three‑dimensional cultures of bone marrow mesenchymal stem cell (BMSC) scaffolds were implanted into mice. According to the shape of the bioceramic scaffolds and the implantation time in vivo, RNA sequencing was performed on the removed scaffolds to explore the molecular mechanism. The in vitro bone plate culture can induce differentiation of chondrocytes, making culture different to that produced in vitro. Implantation of scaffolds in vivo increases the expression of bone‑related genes. The ceramic rod‑like material was found to be superior to the disc shape, and the bone repair effect was more marked with longer implantation times. Gene Ontology analysis revealed that ‘cell chemotaxis’, ‘negative regulation of ossification’ and ‘bone development’ pathways were involved in recovery. It was further confirmed that BMSCs were suitable as seed cells for cartilage tissue engineering, and that the β‑tricalcium phosphate scaffold maybe ideal as cartilage tissue engineering scaffold material. The present research provided new insights into the molecular mechanism of cartilage repair by BMSCs and bioceramic scaffolds. Bioinformatics analysis revealed that AMMECR1L‑like protein, tumor necrosis factor‑induced protein 2, inhibitor of nuclear factor‑B kinase subunit and protein kinase C type and ‘negative regulation of ossification’ and ‘bone development’ pathways may be involved in osteoblast maturation and bone regeneration.
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November-2020
Volume 20 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Huang X, Chen Z, Zhao G, Shi J, Huang G, Chen F, Wei Y, Xia J, Chen J, Wang S, Wang S, et al: Combined culture experiment of mouse bone marrow mesenchymal stem cells and bioceramic scaffolds. Exp Ther Med 20: 19, 2020.
APA
Huang, X., Chen, Z., Zhao, G., Shi, J., Huang, G., Chen, F. ... Wang, S. (2020). Combined culture experiment of mouse bone marrow mesenchymal stem cells and bioceramic scaffolds. Experimental and Therapeutic Medicine, 20, 19. https://doi.org/10.3892/etm.2020.9147
MLA
Huang, X., Chen, Z., Zhao, G., Shi, J., Huang, G., Chen, F., Wei, Y., Xia, J., Chen, J., Wang, S."Combined culture experiment of mouse bone marrow mesenchymal stem cells and bioceramic scaffolds". Experimental and Therapeutic Medicine 20.5 (2020): 19.
Chicago
Huang, X., Chen, Z., Zhao, G., Shi, J., Huang, G., Chen, F., Wei, Y., Xia, J., Chen, J., Wang, S."Combined culture experiment of mouse bone marrow mesenchymal stem cells and bioceramic scaffolds". Experimental and Therapeutic Medicine 20, no. 5 (2020): 19. https://doi.org/10.3892/etm.2020.9147