Open Access

Sarpogrelate and rosuvastatin synergistically ameliorate aortic damage induced by hyperlipidemia in apolipoprotein E‑deficient mice

  • Authors:
    • Hongyang Liu
    • Siwei Xu
    • Guihua Li
    • Dayuan Lou
    • Xiaodan Fu
    • Qin Lu
    • Liman Hao
    • Jingsi Zhang
    • Jiajie Mei
    • Zheng Sui
    • Yu Lou
  • View Affiliations

  • Published online on: October 9, 2020     https://doi.org/10.3892/etm.2020.9300
  • Article Number: 170
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The current study aimed to investigate whether sarpogrelate and rosuvastatin possess anti‑arterial injury, and attempted to elucidate the mechanism of action underlying this activity. Sarpogrelate, a 5‑hydroxytryptamine type 2A antagonist, is extensively used to prevent arterial thrombosis; however, its effects on atherosclerosis remain unknown. In the present study, sarpogrelate combined with rosuvastatin or rosuvastatin alone were administered to male ApoE‑/‑ mice fed a high‑fat diet (HFD) for 8 weeks. Metabolic parameters in the blood samples were analyzed using an automatic analyzer. Aortic tissues were stained with hematoxylin and eosin for morphological analysis. The expression levels of oxidized‑low density lipoprotein (LDL) specific scavenging receptors, lectin‑like oxidized low‑density lipoprotein receptor‑1 (LOX‑1) and cluster of differentiation 68 were detected via immunostaining. mRNA expression levels of interleukin (IL)‑1β, IL‑6 and tumor necrosis factor‑α were determined via reverse transcription‑quantitative PCR analysis, while protein expression levels of LOX‑1 and phosphor(p)‑ERK were determined via western blot analysis. The results demonstrated that sarpogrelate combined with rosuvastatin treatment significantly decreased total cholesterol and LDL cholesterol levels in the serum, and alleviated intimal hyperplasia and lipid deposition, accompanied by decreased inflammatory cell infiltration and lower expression levels of inflammatory cytokines, compared with rosuvastatin monotherapy or HFD treatment. Furthermore, sarpogrelate combined with rosuvastatin treatment significantly decreased the expression levels of LOX‑1 and p‑ERK. Taken together, these results suggest that the positive effects of sarpogrelate combined with rosuvastatin treatment on aortic injury may be associated with the regulation of the LOX‑1/p‑ERK signaling pathway. Sarpogrelate and rosuvastatin synergistically decreased aortic damage in ApoE‑/‑ HFD mice, and thus provide a basis for the treatment of aortic injury caused by hyperlipidemia with sarpogrelate.
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December-2020
Volume 20 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu H, Xu S, Li G, Lou D, Fu X, Lu Q, Hao L, Zhang J, Mei J, Sui Z, Sui Z, et al: Sarpogrelate and rosuvastatin synergistically ameliorate aortic damage induced by hyperlipidemia in apolipoprotein E‑deficient mice. Exp Ther Med 20: 170, 2020.
APA
Liu, H., Xu, S., Li, G., Lou, D., Fu, X., Lu, Q. ... Lou, Y. (2020). Sarpogrelate and rosuvastatin synergistically ameliorate aortic damage induced by hyperlipidemia in apolipoprotein E‑deficient mice. Experimental and Therapeutic Medicine, 20, 170. https://doi.org/10.3892/etm.2020.9300
MLA
Liu, H., Xu, S., Li, G., Lou, D., Fu, X., Lu, Q., Hao, L., Zhang, J., Mei, J., Sui, Z., Lou, Y."Sarpogrelate and rosuvastatin synergistically ameliorate aortic damage induced by hyperlipidemia in apolipoprotein E‑deficient mice". Experimental and Therapeutic Medicine 20.6 (2020): 170.
Chicago
Liu, H., Xu, S., Li, G., Lou, D., Fu, X., Lu, Q., Hao, L., Zhang, J., Mei, J., Sui, Z., Lou, Y."Sarpogrelate and rosuvastatin synergistically ameliorate aortic damage induced by hyperlipidemia in apolipoprotein E‑deficient mice". Experimental and Therapeutic Medicine 20, no. 6 (2020): 170. https://doi.org/10.3892/etm.2020.9300