Open Access

MicroRNA‑208a‑3p promotes osteosarcoma progression via targeting PTEN

  • Authors:
    • Yutuo Fu
    • Yan Wang
    • Ke Bi
    • Lei Yang
    • Yi Sun
    • Boyuan Li
    • Zhenzhong Liu
    • Fulin Zhang
    • Yuan Li
    • Chao Feng
    • Zhenggang Bi
  • View Affiliations

  • Published online on: October 23, 2020     https://doi.org/10.3892/etm.2020.9385
  • Article Number: 255
  • Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteosarcoma (OS) is a malignant bone tumor with a poor prognosis. Accumulated evidence has suggested that microRNAs (miRNAs/miRs) may function as either oncogenes or tumor suppressors, which are associated with tumorigenesis and the progression of different types of cancer. In the present study, the role of miR‑208a‑3p in OS was investigated. The expression levels of miR‑208a‑3p in OS tissues and cell lines were determined via reverse transcription‑quantitative PCR (RT‑qPCR). MTT and colony formation assays were performed to verify the proliferation rate of OS cells. In addition, the effects of miR‑208a‑3p on the migration and invasion of OS cells were revealed using wound‑healing and Transwell assays, respectively. Furthermore, the association between miR‑208a‑3p and phosphatase and tensin homolog (PTEN) 3'‑untranslated region was determined via luciferase reporter assays, western blot and RT‑qPCR analysis. The results indicated that miR‑208a‑3p was upregulated in OS tissues and cell lines compared with adjacent normal tissues and human osteoblastic cells, respectively. miR‑208a‑3p overexpression promoted and miR‑208a‑3p knockdown inhibited OS cells proliferation and metastatic potential. Additionally, PTEN was validated as a direct target of miR‑208a‑3p and its expression was negatively associate with that of miR‑208a‑3p in OS cells. Taken together, these results may suggest that miR‑208a‑3p promoted OS cells proliferation and metastatic potential via targeting PTEN. Therefore, miR‑208a‑3p may be considered as a diagnostic biomarker for OS.
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December-2020
Volume 20 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Fu Y, Wang Y, Bi K, Yang L, Sun Y, Li B, Liu Z, Zhang F, Li Y, Feng C, Feng C, et al: MicroRNA‑208a‑3p promotes osteosarcoma progression via targeting PTEN. Exp Ther Med 20: 255, 2020.
APA
Fu, Y., Wang, Y., Bi, K., Yang, L., Sun, Y., Li, B. ... Bi, Z. (2020). MicroRNA‑208a‑3p promotes osteosarcoma progression via targeting PTEN. Experimental and Therapeutic Medicine, 20, 255. https://doi.org/10.3892/etm.2020.9385
MLA
Fu, Y., Wang, Y., Bi, K., Yang, L., Sun, Y., Li, B., Liu, Z., Zhang, F., Li, Y., Feng, C., Bi, Z."MicroRNA‑208a‑3p promotes osteosarcoma progression via targeting PTEN". Experimental and Therapeutic Medicine 20.6 (2020): 255.
Chicago
Fu, Y., Wang, Y., Bi, K., Yang, L., Sun, Y., Li, B., Liu, Z., Zhang, F., Li, Y., Feng, C., Bi, Z."MicroRNA‑208a‑3p promotes osteosarcoma progression via targeting PTEN". Experimental and Therapeutic Medicine 20, no. 6 (2020): 255. https://doi.org/10.3892/etm.2020.9385