Open Access

Preliminary RNA‑microarray analysis of long non‑coding RNA expression in abnormally invasive placenta

  • Authors:
    • Huishan Zhang
    • Shuzhen Wu
    • Shaoxin Ye
    • Huiting Ma
    • Zhengping Liu
  • View Affiliations

  • Published online on: November 4, 2020     https://doi.org/10.3892/etm.2020.9445
  • Article Number: 13
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding RNAs (lncRNAs) are reported to have important roles in placental development and function, but the role of lncRNAs in abnormally invasive placenta (AIP) remains elusive. In the present study, the differential expression profiles of lncRNAs were analyzed to identify novel targets for further study of AIP. A total of 10 lncRNAs were chosen for validation by reverse transcription‑quantitative PCR. To further determine the functions of dysregulated lncRNAs and their corresponding mRNAs, functional enrichment analysis, coexpression analysis were performed. A total of 329 lncRNAs and 179 mRNAs were identified to be differently expressed between the invasive and control group. Gene ontology analysis revealed that the 10 most significantly enriched functions included upregulated mRNAs and the most significantly enriched term was related to the proteinaceous extracellular matrix (ECM). In the pathway analysis, the two most significantly enriched pathways were the TGF‑β signaling pathway for upregulated mRNAs and the pentose phosphate pathway for downregulated mRNAs. Furthermore, for certain dysregulated lncRNAs, their associated mRNAs were also dysregulated. Of note, BMP and activin membrane‑bound inhibitor and TGF‑β‑induced, as the target genes of the TGF‑β pathway, were indicated to be closely related to the ECM and invasive placental cells. Their nearby lncRNAs G008916 and vault RNA2‑1 were also significantly dysregulated. In conclusion, significant lncRNAs with the potential to serve as biomarkers for AIP were identified.
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January-2021
Volume 21 Issue 1

Print ISSN: 1792-0981
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Copy and paste a formatted citation
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Spandidos Publications style
Zhang H, Wu S, Ye S, Ma H and Liu Z: Preliminary RNA‑microarray analysis of long non‑coding RNA expression in abnormally invasive placenta. Exp Ther Med 21: 13, 2021.
APA
Zhang, H., Wu, S., Ye, S., Ma, H., & Liu, Z. (2021). Preliminary RNA‑microarray analysis of long non‑coding RNA expression in abnormally invasive placenta. Experimental and Therapeutic Medicine, 21, 13. https://doi.org/10.3892/etm.2020.9445
MLA
Zhang, H., Wu, S., Ye, S., Ma, H., Liu, Z."Preliminary RNA‑microarray analysis of long non‑coding RNA expression in abnormally invasive placenta". Experimental and Therapeutic Medicine 21.1 (2021): 13.
Chicago
Zhang, H., Wu, S., Ye, S., Ma, H., Liu, Z."Preliminary RNA‑microarray analysis of long non‑coding RNA expression in abnormally invasive placenta". Experimental and Therapeutic Medicine 21, no. 1 (2021): 13. https://doi.org/10.3892/etm.2020.9445