Open Access

MicroRNA‑301a‑3p increases oxidative stress, inflammation and apoptosis in ox‑LDL‑induced HUVECs by targeting KLF7

  • Authors:
    • Huiqiong Jiang
    • Jiaren Lv
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  • Published online on: March 29, 2021     https://doi.org/10.3892/etm.2021.10001
  • Article Number: 569
  • Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Arteriosclerotic cardiovascular disease is an inflammatory disease of ischemia or endothelial dysfunction caused by atherosclerosis, thereby causing high mortality. The viability and apoptosis of human umbilical vein endothelial cells (HUVECs) following oxidized low‑density lipoprotein (ox‑LDL) induction or transfection was detected by Cell Counting Kit‑8 (CCK‑8) assay and flow cytometry analysis. MicroRNA (miR)‑301a‑3p and Krueppel‑like factor 7 (KLF7) mRNA expression was determined by reverse transcription‑quantitative PCR (RT‑qPCR). The levels of monocyte chemoattractant protein‑1 (MCP‑1) and IL‑6, activities of reactive oxygen species and superoxide dismutase and lactate dehydrogenase leakage were analyzed by respective commercial assay kits. The protein expression of IL‑6, MCP‑1, Bcl2, Bax, poly (ADP‑ribose) polymerase (PARP), cleaved PARP, pro‑caspase3 and cleaved caspase‑3 was detected by western blotting. miR‑301a‑3p expression is highly expressed in ox‑LDL‑induced HUVECs. miR‑301a‑3p is also a target of KLF7. Inhibition of miR‑301a‑3p suppressed oxidative stress, inflammation and apoptosis in ox‑LDL‑induced HUVECs, which was reversed by KLF7 inhibition. In conclusion, miR‑301a‑3p promotes oxidative stress, inflammation and apoptosis in ox‑LDL‑induced HUVECs via decreasing KLF7 expression.
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June-2021
Volume 21 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Jiang H and Lv J: MicroRNA‑301a‑3p increases oxidative stress, inflammation and apoptosis in ox‑LDL‑induced HUVECs by targeting KLF7. Exp Ther Med 21: 569, 2021.
APA
Jiang, H., & Lv, J. (2021). MicroRNA‑301a‑3p increases oxidative stress, inflammation and apoptosis in ox‑LDL‑induced HUVECs by targeting KLF7. Experimental and Therapeutic Medicine, 21, 569. https://doi.org/10.3892/etm.2021.10001
MLA
Jiang, H., Lv, J."MicroRNA‑301a‑3p increases oxidative stress, inflammation and apoptosis in ox‑LDL‑induced HUVECs by targeting KLF7". Experimental and Therapeutic Medicine 21.6 (2021): 569.
Chicago
Jiang, H., Lv, J."MicroRNA‑301a‑3p increases oxidative stress, inflammation and apoptosis in ox‑LDL‑induced HUVECs by targeting KLF7". Experimental and Therapeutic Medicine 21, no. 6 (2021): 569. https://doi.org/10.3892/etm.2021.10001