MicroRNA‑451a plays a role in polycystic ovary syndrome by regulating ovarian granulosa cell proliferation and apoptosis

Yang,Tianjin; Wang,Lie; Zhang,Yun; Zheng,Jindan; Liu,Lili

doi:10.3892/etm.2021.10015

MicroRNA‑451a plays a role in polycystic ovary syndrome by regulating ovarian granulosa cell proliferation and apoptosis

Authors:
- Tianjin Yang
- Lie Wang
- Yun Zhang
- Jindan Zheng
- Lili Liu
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Affiliations: Department of Obstetrics and Gynecology, Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China, Reproductive Center, Qingdao Women and Children's Hospital, Qingdao, Shandong 266000, P.R. China, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China
Published online on: April 2, 2021 https://doi.org/10.3892/etm.2021.10015
Article Number: 583
Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate whether microRNA (miR)‑451a plays a role in polycystic ovary syndrome by regulating the biological function of ovarian granulosa cells and investigate the underlying molecular mechanism. In the present study, reverse transcription‑quantitative PCR (RT‑qPCR) analysis detected markedly low expression of miR‑451a in KGN cells. TargetScan predicted that cyclic AMP‑dependent transcription factor ATF‑2 (ATF2) was a potential target gene of miR‑451a, which was confirmed by a dual-luciferase reporter gene assay. Moreover, western blotting and RT‑qPCR experiments indicated that ATF2 was significantly overexpressed in KGN cells. In addition, western blotting and RT‑qPCR experiments were utilized to assess cell transfection efficiency, and it was found that miR‑451a mimic significantly increased miR‑451a expression in KGN cells. Subsequently, MTT assay was performed to detect cell proliferation and flow cytometry was utilized to detect cell apoptosis. Western blot and RT‑qPCR assays were utilized to assess the protein and mRNA expression of ATF2 and cyclin D1. The results confirmed that miR‑451a mimic significantly decreased ATF2 protein and mRNA expression in KGN cells, and this decrease was reversed by ATF2‑plasmid co‑transfection. Moreover, miR‑451a mimic inhibited cell proliferation, enhanced cell apoptosis, reduced cyclin D1 expression, increased caspase‑3 activity and cleaved caspase‑3 protein levels, while it reduced pro‑caspase 3 protein levels in KGN cells, and these effects were significantly reversed by ATF2‑plasmid. The present preliminary results demonstrated that miR‑451a regulated the proliferation and apoptosis of ovarian granulosa cells by targeting ATF2. Thus, the miR‑451a/ATF2 axis may be a new potential target for the treatment of polycystic ovary syndrome.

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