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Contrast‑enhanced ultrasound molecular imaging of activated platelets in the progression of atherosclerosis using microbubbles bearing the von Willebrand factor A1 domain
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- Published online on: May 3, 2021 https://doi.org/10.3892/etm.2021.10153
- Article Number: 721
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Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
It is now well established that platelet-endothelial interactions not only contribute to atherogenesis in the late stage of atherosclerosis (AS), which is characterized by plaque destabilization and thrombus formation, but also participate in earlier stages of plaque development, through platelet-mediated inflammatory pathways (1). Although a growing body of literature indicates that contrast-enhanced ultrasound (CEU) with targeted microbubbles allows for the detection of molecular and cellular events related to AS inflammation and plaque vulnerability (2), few studies have been performed to explore the role of activated platelets in the procession of AS.
At sites of vascular injury, the initial adhesion of platelets to the endothelium is mediated through interaction between the glycoprotein-Ibα (GPIbα) subunit of the platelet GPIb-IX-V complex and von Willebrand factor (vWF) (3). After binding to exposed subendothelial collagen, vWF multimers undergo conformational change with exposure of the A1 binding domain to GPIbα. This is regarded as the primary adhesive mechanism, helping the platelet attach itself to the target endothelium in the situation of high shear stress generated by blood flow (4,5).
As the binding of the vWF-A1 domain to the platelet GPIbα receptor is the primary adhesive mechanism for platelet-endothelial interactions (6), it was hypothesized that a recombinant protein with an amino acid sequence corresponding to the vWF-A1 domain would be an ideal target ligand in the preparation of targeted microbubbles to activated platelets. In the present study, targeted CEU microbubbles bearing the recombinant vWF-A1 domain were prepared and their use to image platelets-endothelium interaction and assess platelet-mediated inflammation were assessed in flow-chamber experiments and in a murine model of AS.
Materials and methods
Microbubble preparation
Targeted microbubbles were prepared by surface conjugation of biotinylated ligands, as previously described, using a streptavidin bridge (7). Soluble recombinant vWF-A1 domain (amino acids 1238-1481; U-Protein Express BV) was biotinylated at its N terminus for conjugation to the microbubble shell. For this purpose, a molar excess of EZ-Link™ NHS-Biotin (Thermo Fisher Scientific, Inc.) was reacted overnight at 4˚C with the recombinant vWF-A1 domain in phosphate-buffered saline (PBS, pH 8.0). The resulting bioconjugate was purified using Slide-A-Lyzer Dialysis Cassettes (Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. The concentration of the recombinant vWF-A1 domain was determined using an automatic amino acid analyzer (model no. L8900; Hitachi, Ltd.) according to the manufacturer's instructions.
Biotinylated, lipid-shelled decafluorobutane microbubbles were prepared by sonication of a gas-saturated aqueous suspension of distearoylphosphatidylcholine (Avanti Polar Lipids, Inc.), polyoxyethylene-40-stearate (Sigma-Aldrich; Merck KGaA) and distearoylphosphatidylethanolamine-PEG (2000) biotin (Avanti Polar Lipids, Inc.). The size and concentration were assessed by an electrozone sensing cell counter (Multisizer III; Beckman-Coulter,Inc.). Control microbubbles (Mbctrl) were prepared by conjugating biotinylated rat IgG (cat. no. 553880; κ isotype control antibody; BD Biosciences) to microbubbles. Microbubbles targeted to platelets GPIbα (MbA1) were prepared by conjugating biotinylated vWF-A1 to the shell surface. As described in a previous study (8), biotinylated ligands in excess amounts (50 µg per 1x108 microbubbles) were added to occupy all binding sites on the shell of the microbubbles.
In in vitro flow chamber studies, MbA1 and Mbctrl were labeled with dioctadecyloxacarbocyanine (DiO) and dioctadecyltetramethylindocarbocyanine (DiI) perchlorate (Sigma-Aldrich; Merck KGaA), separately.
Flow chamber activated platelets attachment studies Preparation of platelet-rich plasma
Blood samples were obtained from 20 random healthy human volunteers (age range, 20-50 years; mean age, 35±10 years; 11 males; 9 females) at the Department of Ultrasound, Tongji Hospital from March 2015 to May 2015. A standardized technique of double centrifugation (9) was used to prepare platelet-rich plasma (PRP). Automatic hemocyte analyzer (model no. XS-800i; Sysmex Corporation) was used to count platelets in PRP. The platelet recovery rate was the total amount of platelets obtained in PRP compared to the number in whole blood. PRP smears were stained with Wright Giemsa (10) to reveal the purity of platelet suspensions. The present study was approved by Tongji Hospital Ethics Committee and written informed consent was obtained from all volunteers before the study.
Coating and activation of platelets. Parallel plate culture dishes with a diameter of 35 mm were incubated with collagen type I (100 µg/ml; Beijing Solarbio Science & Technology Co., Ltd.) at 4˚C overnight and then blocked with bovine serum albumin (5 mg/ml; Wuhan Servicebio Technology Co., Ltd.) at room temperature for 1 h. PRP (platelet concentration, 46x106/ml) and platelet activator was added and incubated at room temperature for at least 15 min. To prepare the platelet activator, 1 ml of 10% calcium chloride was mixed with 500 U of bovine thrombin (Beijing Solarbio Science & Technology Co., Ltd). Indirect immunofluorescence was performed using a rat monoclonal primary antibody against mouse P-selectin (cat. no. CD62P; BD Biosciences) and goat anti-rat Alexa Fluor 488 secondary antibody (Invitrogen; Thermo Fisher Scientific, Inc.) to identify activated platelets.
Flow chamber studies. In vitro flow chamber studies were performed at room temperature without specific CO2 conditions to test the binding of microbubbles to activated platelets under different flow conditions. The parallel plate culture dishes coated with activated platelets were mounted on a parallel plate flow chamber (GlycoTech Corporation) with a gasket thickness of 0.01 in and a channel width of 2.5 mm. To maintain the consistency of the activated platelets on culture dishes, PRP of the same concentration (46x106/ml) was used in each condition. An aqueous suspension of MbA1 labeled with DiO (6x106/ml) or Mbctrl labeled with DiI (6x106/ml) was drawn through the flow chamber with an adjustable withdrawal pump to generate a flow shear stress of 2.0, 4.0, 6.0 or 8.0 dynes/cm2, respectively. After 5 min of continuous infusion, freely circulating microbubbles in the chamber were removed by a 2 min PBS rinse at a low shear stress of 0.1 dynes/cm2. The number of microbubbles adhered to platelets were averaged from 10-15 randomly selected nonoverlapping optical fields under a fluorescent microscope with a 40x objective. Experiments were performed at least in triplicate for each condition.
CEU molecular imaging Animal models
The study was approved by the Animal Care and Use Committee of Tongji Medical College, Huazhong University of Science and Technology. A total of 20 male wild-type C57Bl/6 mice and 31 apolipoprotein E deficient (ApoE-/-) mice with age-dependent atherosclerosis produced on a C57Bl/6 background were studied at 8, 16 and 32 weeks of age (n=8-12 for each strain at each age; Beijing Vital River Laboratory Animal Technology Co., Ltd.). Upon purchase, the mice were 4 weeks of age and weighed ~9 g. As a control, C57Bl/6 mice were fed on a chow diet and from 5 weeks of age onwards, while ApoE-/- mice were fed on a hypercholesterolemic diet (HCD) containing 21% fat by weight, 0.15% cholesterol, and 19.5% casein without sodium cholate. All mice were fed with free food and water access, and were raised in the Animal Experimental Center of Tongji Medical College, Huazhong University of Science and Technology, which had a specifical pathogen free barrier environment. Animals were housed at a temperature of 20-26˚C and a relative humidity of 40-70%, under a 10/14 h light/dark cycle.
CEU molecular imaging. Mice were anesthetized with inhaled isoflurane (1.5%). The jugular vein was cannulated for microbubble administration. Imaging of the proximal ascending aorta was performed with an ultrahigh frequency (25 MHz) mechanical sector imaging system. CEU was performed with Contrast Pulse Sequencing, which detects the nonlinear fundamental signal component for microbubbles. At each injection, images were acquired (MI 0.16) 8 min after intravenous injection of targeted or control microbubbles (3x106/ml; 2.2±1.7 µm) performed in random order. Microbubbles in the sector were then destroyed by increasing the mechanical index to 1.0 for 0.5 sec. Subsequent post-destruction images were acquired at a mechanical index of 0.16. In order to test the specificity of MbA1 to activated platelets on the endothelium, in vivo blocking experiments were performed with an additional group of 3 ApoE-/- mice at 32 weeks of age. CEU molecular imaging of platelets with MbA1 and Mbctrl was performed in these mice before and after administration of 1.5 µg/g GpIbα antibody (cat. no. R300, Emfret Analytics GmbH & Co. KG) by intraperitoneal injection, which can result in 95% platelet immune depletion.
Image analysis. Signals from microbubbles were quantitatively estimated by commercially available software (Vevo 2100 imaging analysis software; FUJIFILM Visual Sonics, Inc.). During CEU imaging, 8 min after intravenous injection of microbubbles, the first 20 pre-destruction contrast frames were used to derive the total quantity of microbubbles, including retained and freely circulating microbubbles. To determine the signal from retained microbubbles alone, all microbubbles in the region of interest were destroyed and the subsequent 20 post-destruction contrast frames represented the freely circulating microbubbles. Therefore, the signal from targeted retained microbubbles was calculated by digitally subtracting 20 averaged post-destruction contrast frames from 20 averaged pre-destruction frames. Intensity was measured from a region-of-interest placed on the aorta arch.
Histology
After imaging, the 10% neutral formalin perfusion-fixed short-axis sections from the ascending aorta in ApoE-/- mice were evaluated in all study groups. Masson trichrome staining (11) was performed for assessment of plaque morphometry and the severity of atherosclerotic lesions. Immunohistochemistry for platelet expression was performed with a rabbit polyclonal primary antibody against GPIIb (cat. no. Ab63983; Abcam) and species-appropriate Alexa Fluor-594 secondary antibody (Invitrogen; Thermo Fisher Scientific, Inc.) was used.
Statistical analysis
Data were analyzed using SPSS (version 17.0; SPSS, Inc.), all parameter data were tested for normality and homogeneity of variance. The Shapiro Wilk test was used to verify whether continuous variables met the normal distribution, and all the data were presented as the mean ± SD. Comparisons of in vitro microbubble adhesion at different shear conditions were performed using one-way ANOVA method followed by Bonferroni multiple comparison tests. Independent t-tests were used to compare the number of attached microbubbles and the signal enhancements between MbA1 and Mbctrl. Comparisons of the signal enhancements in the different age cohorts within the same animal group were made with one-way ANOVA and Bonferroni for multiple comparison tests. P<0.05 was considered to be statistically significant.
Results
Flow chamber activated platelets attachment studies Preparation of PRP
PRP was prepared with a concentration of 399x109/l. The platelet recovery rate was 167.65%, which exceeded 100%. Under microscopy, platelets with a high purity were seen on PRP smears stained with Wright Giemsa (Fig. S1A).
Platelet activation. Under the microscope, activated platelets were seen in a state of aggregation on the parallel plate culture dishes (Figs. S1B and S2A). Indirect immunofluorescence revealed a CD62 positive green fluorescent signal from activated platelets (Fig. S2B).
Flow chamber studies. Under light microscopy, activated platelets coating the parallel plate culture dishes were seen to be aggregated into irregular clusters (Fig. 1A and C). Under fluorescence microscopy, DiO labeled MbA1 appeared selectively attached to activated platelet aggregates (Fig. 1B; green areas). However, DiI labeled Mbctrl (red areas), were sparse and not related to the aggregated platelets (Fig. 1D), which indicated nonspecific binding to the platelets. Across a range of shear stresses, attachment of MbA1 to activated platelet aggregates was significantly higher than that of Mbctrl lacking a targeting ligand (P<0.05 at each shear stress; Fig. 2). With the shear stress increased from 2.0 to 8.0 dynes/cm2, the number of attached MbA1 remained constant (P>0.05; Fig. 2). There was no statistical difference (P>0.05) in the quantity of attached Mbctrl from 2.0 to 8.0 dynes/cm2.
Molecular imaging of platelet adhesion
In ApoE-/- mice, CEU molecular imaging of the proximal ascending aorta detected selective signal enhancement from MbA1 compared to Mbctrl at 8, 16 and 32 weeks of age (Fig. 3). Imaging signals from MbA1 increased from 8 to 32 weeks of age (P<0.05). Signals from Mbctrl were low and similar between groups. In C57Bl/6 mice, there was no statistical difference in imaging signal between Mbctrl and MbA1 (Fig. 3). After in vivo platelet immune depletion in 3 ApoE-/- mice at 32 weeks of age, the selective signal of MbA1 decreased significantly (P<0.05), from 10.71 before blocking to 2.2 after blocking.
Immunohistochemistry
In ApoE-/- mice, the plaque lesions progressed from 8-32 weeks. There was mild intimal thickening in aorta sections at 8 weeks of age. Small but discrete fibrous plaques were seen at 16 weeks of age. At 32 weeks of age, large plaques with a lipid-rich core and a necros region were seen in all the sections, and these lesions tended to protrude into the aortic lumen (Fig. 4). Immunohistochemistry for GPIIb revealed the presence of platelets on the endothelial cell surface. In ApoE-/- mice, minimal and local GPIIb expression were present on the intimal surface of the aorta at 8 and 16 weeks of age (Fig. 4). With plaque progression, abundant GPIIb expression was detected on the intimal surface and in atherosclerotic lesions at 32 weeks of age (Fig. 4).
Discussion
Molecular imaging of inflammation with ultrasonography has been achieved by surface modification of microbubble contrast agents with ligands targeted to specific molecular and cellular events (12). Kaufmann et al (13). successfully applied vascular cell adhesion molecule-1 targeted microbubbles to detect vascular inflammatory responses in apolipoprotein E deficiency mice with atherosclerotic lesions. McCarty et al (14) used GPIbα antibody as a targeting moiety and noninvasive molecular imaging could detect activated vWF on the vascular endothelium, which further contributed to reveal an advanced prothrombotic and inflammatory phenotype in atherosclerotic disease.
It is widely accepted that platelets play a significant role in thromboembolic complications of advanced atherosclerotic lesions (15) and research attention is focused om platelet involvement in the formation of atherosclerotic lesions through platelet-endothelial interactions (16). In the present study, targeted CEU microbubbles bearing a recombinant vWF-A1 domain were prepared and their specific adhesion to activated platelets in a model flow chamber system and in a murine model of AS demonstrated. With these targeted microbubbles, the potent role of activated platelets in the process of AS can be further explored with CEU imaging.
Molecular imaging with CEU relies on the selective targeting and retention of acoustically active contrast agents at sites of disease (17). In the production of targeted microbubbles for molecular imaging, a typical strategy is to attach disease-specific ligands, including monoclonal antibodies, peptides, glycoproteins and other small molecules to the microbubble shell surface (18). A number of molecular imaging studies have focused on the attachment of monoclonal antibodies on the microbubbles because of their easy availability (19,20). However, there have been difficulties concerning the firm adhesion of microbubbles combined with antibodies under shear flow (21). The slow association kinetic properties and the high molecular weight of antibodies could be the reasons that reduce the capture efficiency on the target of interest. In previously studies on platelet targeting, targeted microbubbles were outfitted with monoclonal antibodies against GPIIb/IIIa (3,6). Unfortunately, the specific adhesion of these platelet targeted microbubbles was only observed under static conditions or dynamic flow conditions with low shear stress.
An increasing number of more studies have focused on preparation of targeted microbubbles with smaller peptide or peptide mimetic ligand molecules (22-24). Peptides, including polymeric sialyl Lewis X oligosaccharide derivatives (23) and short glycosulfopeptides (24), have been confirmed to provide efficient microbubble targeting even in fast flow. In the process of AS, platelet GPIbα plays a critical role in platelet adhesion and aggregation under high-shear conditions (7). GPIbα is a component of the GPIb-V-IX complex and initiates platelet adhesion by binding to collagen bound vWF (5). vWF is synthesized by endothelial cells and has multiple A, C and D type domains (25). In human blood flow, when the shear stress exceeds 400 dynes/cm2, active A1 domains become exposed and can bind to GPIbα, acting as a hook for the capture of platelets and contributing to platelet adhesion under high vascular shear stress (26,27). The pivotal role of vWF-platelet interaction in mediating platelet adhesion under high-shear conditions indicated that the A1 domain of vWF could be a potential ligand used to prepare microbubbles targeted to platelets.
In the present study, a recombinant protein with an amino acid sequence corresponding to the A1 domain of vWF was used as the specific targeting ligand attached to the microbubble surface. The present study results confirmed the specific attachment of MbA1 to platelet aggregates under dynamic flow chamber conditions.
Differing from previous flow chamber studies, in which the specific attachment of targeted microbubbles conjugated to antibodies decreased with increasing shear stress, the present flow chamber experiment revealed that the number of MbA1 binding to platelet aggregates remained constant when shear stress increased from 2.0 to 8.0 dynes/cm2. The present results may provide insight into the high-strength interactions between platelets and vWF-A1 domain under dynamic flow conditions. In a study on a molten globule intermediate of the von Willebrand factor, the A1 domain firmly tethers platelets under shear flow. Tischer et al (28) suggested that the mean platelet pause times increased from ~0.75 sec at a shear stress of nearly 2.0 dynes/cm2 to 0.9 sec at 8.0 dynes/cm2, and then decreased again upon further increase of the shear stress. Pause times determined the average length of time a platelet was immobile, which has a complex dependence on the shear stress (28). With high shear stress exposing the vWF-A1 domain expressed on the endothelium, the active A1 domains become firmly bound to platelets. This behavior is referred to as a catch-slip interaction where the bond initially becomes weaker at low force, strengthens at intermediate forces and weakens again at higher forces (29,30), which could be the reason for the stable interactions between MbA1 and platelets in the conditions of increasing shear stress from 2.0 to 8.0 dynes/cm2. To the best of our knowledge, the targeted microbubbles bearing vWF-A1 domain prepared in the present study have higher affinities to platelets, which may produce favorable binding kinetics of microbubbles under high shear forces. Additionally, the vWF-A1 domain as a small molecular peptide is less immunogenic in humans, which may provide a better safety profile in future clinical application (31).
AS is a chronic inflammatory disease and various complex processes contribute to its pathophysiology and the development of the atherosclerotic plaque over decades (32,33). While it is now well established that platelets play a critical role in thrombotic complications of advanced AS, such as rupture of the vulnerable plaque, a growing experimental literature has established that platelets participate in the initiation of the atherogenic process through platelet-endothelium interaction (34). In vivo study of labeled platelets indicated that substantial numbers of platelets adhered to the carotid endothelium before the development of manifest atherosclerotic lesions in ApoE-/- mice (35). Consistent with these studies, using CEU molecular imaging in the present study, the presence of activated platelets on the vascular endothelium at the early lesion-prone stage of AS in in vivo experiments was confirmed. Moreover, there was also an age-dependent increase in selective signal enhancement from microbubbles bearing the A1 domain, indicating a relationship between the degree of platelet adhesion and disease severity. Platelets interacting with the endothelium may influence the development and progression of AS through a variety of mechanisms (36,37).
The intact nonactivated endothelium represents a natural barrier preventing platelet adhesion to the extracellular matrix. However, platelets can adhere directly to the intact but activated endothelial cell monolayer via GPIb/P-selectin or P-selectin glycoprotein ligand (PSGL)1/P-selectin (38). After contact between platelets and the endothelium has been established, platelets are activated and release proinflammatory cytokines and chemoattractants and express CD40 ligand to further induce the proatherogenic phenotype of endothelial cells (39,40). In this manner, adherent platelets enhance the recruitment of leukocytes, progenitor cells and dendritic cells to the vascular wall and tissues in the process for AS (41). Platelets not only promote an inflammatory response in leukocytes and endothelial cells, but may also themselves respond to inflammatory mediators produced by these cells. Studies have demonstrated that the bidirectional interaction between platelets and leukocytes and endothelial cells involves both inflammatory and prothrombotic pathways, contributing to a pathogenic loop in AS and plaque destabilization (37,42). Our application of CEU molecular imaging to evaluate the biological process of activated platelets in AS not only contribute to understanding the inflammatory role of platelets in AS, also can be used to explore the effects of new anti-platelets therapy in preventing AS.
The present study had several limitations. Firstly, the selective attachment of targeted microbubbles to activated platelets was explored in a parallel plate chamber. However, the flow in parallel plate chamber may not accurately simulate hemodynamics in the vessel to assess the binding efficiency of microbubbles in vivo. Secondly, the specific attachment to activated platelets was not directly compared between microbubbles bearing the vWF-A1 domain and those bearing antibodies. However, in the flow chamber studies with targeted microbubbles conjugated to monoclonal antibodies, the specific attachment of the targeted microbubbles decreased with increasing shear stress. Thirdly, though CEU molecular imaging was used to assess the extent of activated platelets in the process of atherogenesis, the underlying mechanisms contributing to the inflammatory progress were not explored in the present study.
Supplementary Material
PRP smears with Wright Giemsa staining. (A) A large number of platelets presenting normal morphology (black arrow) were observed on PRP smears. Several red blood cells (white arrowhead) and neutrophils (black arrowhead) were occasionally seen surrounding the platelets. (B) After coating and activation of PRP, aggregated platelets (yellow arrow) and collagen (red arrowhead) were observed on parallel plate culture dishes. PRP, platelet rich plasma.
Identification of platelet activation. (A) Aggregated platelets (black arrow) were observed on the parallel plate culture dishes. (B) CD62 positive green fluorescent signals were detected from activated platelets (white arrow).
Acknowledgements
Not applicable.
Funding
Funding: This research was supported by grants from the National Science Foundation of China (grant no. 81371581) and from the Natural Science Foundation of Hubei Province in China (grant no. 2019CFB691).
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Authors' contributions
YL, JT and HL designed the experiments and wrote the manuscript. JT, YW, RS, YZ and JZ performed experiments and analyzed data. YL, HL and YZ assessed all the raw data and confirmed its authenticity and legitimacy. All authors discussed the results and reviewed and approved the final manuscript.
Ethics approval and consent to participate
All animal experimental procedures were approved by the Animal Care and Use Committee of Tongji Medical College, Huazhong University of Science and Technology (Wuhan, China). Experiments involving human tissues were approved by Tongji Hospital Ethics Committee and volunteers provided their informed consent.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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