miR‑3677‑5p promotes the proliferation, migration and invasion of hepatocellular carcinoma cells and is associated with prognosis

Mao,Hai-Xiang; Chen,Bai-Wen; Wang,Jie; Ma,Chen-Yang; Gan,Yi-Chao; Qiu,Kai-Jie

doi:10.3892/etm.2021.10212

miR‑3677‑5p promotes the proliferation, migration and invasion of hepatocellular carcinoma cells and is associated with prognosis

Authors:
- Hai-Xiang Mao
- Bai-Wen Chen
- Jie Wang
- Chen-Yang Ma
- Yi-Chao Gan
- Kai-Jie Qiu
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Affiliations: Department of Pancreatic and Hepatobiliary Surgery, Ningbo Medical Treatment Center, Lihuili Hospital, Ningbo, Zhejiang 315000, P.R. China
Published online on: May 19, 2021 https://doi.org/10.3892/etm.2021.10212
Article Number: 780
Copyright: © Mao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNA (miRNA/miR)‑3677 has been indicated to be negatively associated with the survival of patients with hepatocellular carcinoma (HCC) based on The Cancer Genome Atlas database. However, as a novel miRNA, the role of miR‑3677‑5p in HCC has remained to be elucidated. In the present study, the expression of miR‑3677‑5p was assessed in HCC tissues and cell lines using reverse transcription‑quantitative PCR. Survival analysis was performed using Kaplan‑Meier curves. Furthermore, the prognostic significance of miR‑3677‑5p was evaluated using Cox regression analysis. The effects of miR‑3677‑5p on cell proliferation, as well as migration and invasion capacities, were analyzed using Cell Counting Kit‑8, crystal violet and Transwell assays. The results demonstrated that the level of miR‑3677‑5p expression was upregulated in human HCC tissues and cell lines and that miR‑3677‑5p expression was closely associated with tumor size, TNM stage and vascular invasion. Furthermore, high miR‑3677‑5p expression was significantly associated with unfavorable clinical prognosis for patients with HCC. Overexpression of miR‑3677‑5p was indicated to significantly promote the proliferation, migration and invasion of HCC cells, whereas knockdown of miR‑3677‑5p was observed to have an inhibitory effect. In conclusion, the present study demonstrated that miR‑3677‑5p acts as an oncogene that has a critical role in the regulation of HCC proliferation and progression. Hence, miR‑3677‑5p may serve as a valuable prognostic biomarker and may be developed as a promising therapeutic target for HCC.

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