STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells

Zhang,Lu; Lu,Shun-Yuan; Guo,Rui; Ma,Jin-Xia; Tang,Ling-Yun; Wang,Jin-Jin; Shen,Chun-Ling; Lu,Li-Ming; Liu,Jie; Wang,Zhu-Gang; Zhang,Hong-Xin

doi:10.3892/etm.2021.10283

STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells

Authors:
- Lu Zhang
- Shun-Yuan Lu
- Rui Guo
- Jin-Xia Ma
- Ling-Yun Tang
- Jin-Jin Wang
- Chun-Ling Shen
- Li-Ming Lu
- Jie Liu
- Zhu-Gang Wang
- Hong-Xin Zhang
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Affiliations: Research Center for Experimental Medicine, State Key Laboratory of Medical Genomics, Shanghai Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China, Shanghai Model Organisms Center, Shanghai 201321, P.R. China, Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China, Shanghai Institute of Orthopaedics and Traumatology, Shanghai Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China
Published online on: June 8, 2021 https://doi.org/10.3892/etm.2021.10283
Article Number: 851

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Abstract

Prostate cancer (PCa) is one of the most common types of cancer and is a serious threat to men's health due to the high rate of incidence and metastasis. However, the exact underlying pathology of this malignant disease has yet to be fully elucidated. The ezrin‑radixin‑moesin (ERM) family of proteins are associated with the development and metastasis of various types of cancer. Serine threonine kinase 10 (STK10) is an ERM kinase that is involved in the activation of ERM proteins and serves essential roles in the aggregation and adhesion of lymphocytes. To evaluate the functional roles of STK10 in the pathogenesis of PCa, a STK10‑knockout (KO) DU145 PCa cell line was generated using the CRISPR‑Cas9 gene editing system, and the effects of STK10 deletion on tumor biological behaviors were further analyzed. The present data suggested that STK10 KO promoted PCa cell proliferation by inhibiting p38 MAPK activation and suppressed migration primarily via the inhibition of p38 MAPK signaling and ERM protein activation. To the best of our knowledge, this is the first study to provide evidence that STK10 plays important roles in the proliferation and migration of PCa cells, which will be useful for further investigation into the pathogenesis of this disease.

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