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cRGDyK‑modified procaine liposome inhibits the proliferation and motility of glioma cells via the ERK/p38MAPK pathway
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- Published online on: June 9, 2021 https://doi.org/10.3892/etm.2021.10291
- Article Number: 859
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Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Glioma is a common primary tumor of the central nervous system (1). The incidence of glioma is increasing year by year, with an annual growth rate of ~1.2% (2). Glioma is a serious threat to human life and health. Despite the emergence of treatment regimens comprising combined surgery and chemoradiotherapy, the prognosis of patients with malignant glioma has not been markedly improved and the median survival time is only 12-15 months (3,4). Since glioma usually grows and infiltrates surrounding tissue and its boundary is not clear, it is challenging to remove completely by surgery (5). Therefore, the use of targeted therapy is therapeutically important (6). A variety of targeted drugs have been used clinically or tested for the treatment of glioma in clinical trials; however, the development of more effective therapeutic drugs is necessary in order to improve the prognosis of patients (7).
The antitumor effects and mechanisms of certain anesthetics have been revealed (8). Studies have demonstrated that anesthetics affect the apoptosis, proliferation, metastasis and DNA demethylation of some types of cancer cells (9-11). Anesthetics have exhibited antitumor effects on various malignancies. For example, studies have shown that clinical doses of lidocaine and bupivacaine can induce the apoptosis of MCF-7 breast cancer cells, while dibucaine has a pro-apoptotic effect on HL-60 promyelocytic leukemia cells (10,11). In addition, procaine is able to inhibit the proliferation of CNE-2Z nasopharyngeal carcinoma cells (12).
The p38 MAP kinase (MAPK) participates in a signaling pathway that mediates the response of cells to cytokines and stress (13). The involvement of the ERK/p38 MAPK pathway in the progression of several types of cancer, including glioma, has already been revealed (14). Several oncogenes have been shown to affect the progression and metastasis of multiple types of cancer, including breast, lung and gastric cancer, through this pathway, and numerous drugs targeting the ERK/p38MAPK pathway have been developed or are in clinical trials (15).
Strategies for the effective delivery of anesthetics, such as procaine, into the brain are of great interest for the treatment of glioma. Researchers have attempted to increase the penetration of the blood-brain barrier (BBB) by drugs, and active targeting is proving to be an attractive approach for the enhancement of glioma-targeting ability using specific ligands (16,17). The pentapeptide cyclic (arginine-glycine-aspartic acid-tyrosine-lysine) (cRGDyK) is a promising ligand (18,19) for targeting the integrin αvβ3 receptor, which is highly expressed on the surface of multiple types of cancer cells, including C6 glioma and MDA-MB-231 breast cancer cells (20-22). As the expression of integrin αvβ3 on normal cells is limited, it is an attractive receptor for glioma targeting. In addition, the integrin receptor is highly expressed on cerebral capillary endothelial cells (23). Therefore, the cRGDyK peptide may have the ability to cross the BBB and target glioma.
To verify the aforementioned functions of cRGDyK peptide and investigate the antitumor mechanism of procaine, a procaine-loaded liposome modified with cRGDyK (Pro/cRGDyK-L) was developed in the present study. The ability of the cyclic peptide to enhance the penetration of the BBB by procaine and improve cellular uptake was investigated. Additionally, the inhibitory effects of Pro/cRGDyK-L on cell migration, apoptosis and cell cycle arrest were evaluated. Furthermore, whether Pro/cRGDyK-L targets the ERK/p38 MAPK pathway and inhibits tumor growth in vivo was analyzed.
Materials and methods
Chemistry
Synthesis of (3S,10R,13R,17R)-10,13-dimethyl-17-((R) -6-methylheptan-2-yl)-2,3,4,7,8,9,10,11,12,13,14,15,16,17- tetradecahydro-1H-cyclopenta[a]phenanthren-3-yl 4-methyl benzenesulfonate (compound 2). To a solution of cholesterol (compound 1; 1.00 g, 2.59 mmol) in pyridine (5 ml), a solution of p-toluenesulfonyl chloride (0.74 g, 3.88 mmol) in pyridine (5 ml) was added. After stirring the mixture for 10 h at 50˚C, the solvent was removed and the residue was redissolved in ethyl acetate (20 ml). The mixture was washed with 1 mol/l HCl and saturated NaCl. The solvent was then removed to yield compound 2 (1.19 g, 85%), which was used for the next step without further purification. Melting point: 127-129˚C. High-resolution mass spectrometry (HRMS): Electrospray ionization (ESI+) calculated for C34H52O3SNa [M+Na]+, 563.3535; found, 563.3532. Elemental analysis: Calculated, C, 75.51; H, 9.69; S, 5.93; found, C, 75.43; H, 9.78; S, 6.11.
Synthesis of 23-(((3R,10R,13R,17R)-10,13-dimethyl-17- ((R)-6-methylheptan-2-yl)-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-yl)oxy)- 3,6,9,12,15,18,21-heptaoxatricosan-1-ol (compound 3). To a solution of compound 2 (1.00 g, 1.85 mmol) in dioxane (15 ml) was added octaethylene glycol (6.85 g, 18.49 mmol), and the reaction mixture was refluxed for 20 h. The solvent was then removed and the residue was redissolved in dichloromethane (CH2Cl2; 30 ml). The mixture was washed with saturated NaCl. After removing the CH2Cl2, the residue was purified by chromatography to give compound 3 (0.66 g, 48%) as a colorless oil. HRMS: (ESI+) calculated for C43H78O9Na [M+Na]+, 761.5544; found, 761.5540. Elemental analysis: Calculated, C, 69.88; H, 10.64; found, C, 69.76; H, 10.79.
Synthesis of tert-butyl 26-(((3R,10R,13R,17R)-10, 13-dimethyl-17-((R)-6-methylheptan-2-yl)-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-yl)oxy)-3,6,9,12,15,18,21,24-octaoxahexacosanoate (compound 4). To a solution of compound 3 (0.50 g, 0.68 mmol) in toluene (10 ml) was added 50% NaOH aqueous solution (5 ml) at 0˚C. Then, tert-butyl bromoacetate (0.16 ml, 1.02 mmol) and tetra-n-butylammonium hydrogen sulfate (24 mg, 0.07 mmol) were added to the solution and the mixture was stirred for 20 h at ambient temperature. The organic layer was separated and washed with saturated NaCl. After removing the solvent, the residue was purified by chromatography to give compound 4 (0.44 g, 75%) as a colorless oil. HRMS: (ESI+) calculated for C49H88O11Na [M+Na]+, 875.6224; found, 875.6227. Elemental analysis: Calculated, C, 68.98; H, 10.40; found, C, 68.85; H, 10.50.
Synthesis of 26-(((3R,10R,13R,17R)-10,13-dimethyl-17- ((R)-6-methylheptan-2-yl) -2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-yl)oxy)- 3,6,9,12,15,18,21,24-octaoxahexacosanoic acid (compound 5). To a solution of compound 4 (1.50 g, 1.76 mmol) in toluene (20 ml) was added p-toluenesulfonic acid (60 mg, 0.35 mmol), and the mixture was heated to reflux for 10 h. The solvent was removed and the residue was purified by chromatography to give compound 5 (1.23 g, 88%) in the form of a yellow oil. HRMS: (ESI+) calculated for C45H80O11Na [M+Na]+, 819.5598; found, 819.5595. Elemental analysis: Calculated, C, 67.81; H, 10.12; found C, 67.89; H, 10.03.
Synthesis of 2,5-dioxopyrrolidin-1-yl 26-(((3R,10R,13R, 17R)-10,13-dimethyl-17-((R)-6-methylheptan-2-yl) -2,3,4,7,8,9,10,11,12,13,14,15,16,17- tetradecahydro-1H-cyclopenta[a]phenanthren-3-yl)oxy)-3,6,9,12,15,18,21,24-octaoxahexacosanoate (compound 6). To a solution of compound 5 (0.80 g, 1.00 mmol) in tetrahydrofuran (THF; 10 ml) was added N-hydroxysuccinimide (NHS; 0.35 g, 3.00 mmol) and N,N-dicyclohexylcarbodiimide (DCC; 0.62 g, 3.00 mmol), and the mixture was stirred at room temperature for 20 h. After removing the THF, the residue was purified by chromatography to give compound 6 (0.58 g, 65%) as a yellow oil. HRMS: (ESI+) calculated for C49H83O13Na [M+Na]+, 916.5762; found, 916.5766. Elemental analysis: Calculated, C, 65.82; H, 9.36; N, 1.57; found, C, 65.88; H, 9.45; N, 1.42.
Synthesis of 2-((2R,5S,8R,11R)-8-(1-(((3R,10R,13R,17R)- 10,13-dimethyl-17-((R)-6-methylheptan-2-yl)-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-yl)oxy)-26-oxo-3,6,9,12,15,18,21,24-octaoxa- 27-azahentriacontan-31-yl)-11-(3-guanidinopropyl)- 5-(4-hydroxybenzyl)-3,6,9,12,15-pentaoxo-1,4,7,10, 13-pentaazacyclopentadecan-2 -yl)acetic acid (cRGDyK-modified ligand). To a solution of compound 6 (0.20 g, 0.22 mmol) and cRGDyK (0.14 g, 0.22 mmol) in 10 ml dimethylformamide was added triethylamine (0.16 ml, 1.12 mmol), and the mixture was stirred for 5 h at room temperature. The reaction was terminated by adding saturated NaCl (50 ml) and the mixture was extracted with CH2Cl2 three times. After removing CH2Cl2, the residue was purified by chromatography to provide the cRGDyK-modified ligand (0.15 g, 48%) as a yellow oil. HRMS: (ESI+) calculated for C72H119N9O18Na [M+Na]+, 1,420.8571; found, 1,420.8576. Elemental analysis: Calculated, C, 61.82; H, 8.58; N, 9.01; found, C, 61.89; H, 8.65; N, 9.14.
Preparation and characterization of liposomes
The procaine-loaded liposomes were prepared using the thin film hydration method (24). The component ratio was optimized as follows: i) conventional liposome (L), soybean phosphatidyl choline (Shanghai Pharmaceutical Co., Ltd.)/cholesterol molar ratio 62:33; ii) cRGDyK-modified liposome (cRGDyK-L), SPC/cholesterol/ligand molar ratio 62:33:3. In brief, all the lipid materials were dissolved in a mixed solvent comprising CH2Cl2 and methanol (2:1 v/v), and then heated to 37˚C on a rotary evaporator to remove the solvent and form a thin film. After drying in a vacuum for 10 h, the film was hydrated with PBS (pH 7.4) at 20˚C for 30 min and then sonicated intermittently at 80 W for 80 sec to obtain the liposomes. An appropriate amount of procaine (weight ratio, 1:30) or 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-carboxyfluorescein (CFPE) (final concentration, 20 µg/ml) was added to the solution of lipid liposomes before removing the solvent to prepare procaine-loaded liposomes (Pro/L and Pro/cRGDyK-L) or CFPE-labeled liposomes (CFPE/L and CFPE/cRGDyK-L).
The entrapment efficiency [EE (%)] and drug loading efficiency [DL (%)] of procaine were determined by high performance liquid chromatography (HPLC). The detection was performed using an Agilent 1200 HPLC system (Agilent Technologies, Inc.) with an Ultimate LP-C18 column (4.6x250 mm, 5 µm; Sepax Technologies, Inc.) at 25˚C. The mobile phase comprised water and methanol (68:32 v/v) with a flow rate of 1.0 ml/min. For testing, 10 µl procaine-containing sample was injected and the detection wavelength was 290 nm. The EE (%) and DL (%) were calculated according to the following equations: EE (%) = weight of encapsulated procaine / total weight of procaine, and DL (%) = weight of encapsulated procaine / total weight of liposome. In addition, the size and ζ potential of Pro/L and Pro/cRGDyK-L were detected using a Malvern Zetasizer Nano ZS90 (Malvern Panalytical).
Release by Pro/L and Pro/cRGDyK-L in vitro
The release behavior of procaine from the Pro/L and Pro/cRGDyK-L liposomes was evaluated via dialysis. The release behavior of procaine from free procaine was also analyzed as a control. Briefly, the liposomes or free procaine were put into a dialysis bag (molecular weight cut-off, 8,000-14,000 Da) with 50 ml PBS containing 0.1% Tween 80 (v/v) as release medium. The bags were then shaken at 37˚C with gentle oscillation (45 rpm). At predetermined time points between 0 and 48 h, 0.1 ml sample was taken out and replaced with fresh medium. Then, the amount of procaine was tested using the aforementioned HPLC method.
Stability of Pro/L and Pro/cRGDyK-L in vitro
The stability of the Pro/L and Pro/cRGDyK-L liposomes was determined by investigating turbidity variations in the presence of fetal bovine serum (FBS, Gibco; Thermo Fisher Scientific, Inc.). In general, each of the liposome formulations was mixed with an equal volume of FBS and then maintained at 37˚C with continuous shaking (50 rpm). The transmittance at 750 nm was measured between 0 and 48 h using a microplate reader (Bio-Rad model 550; Bio-Rad Laboratories, Inc.) and was evaluated relative to the transmittance of PBS, which was defined as 100%.
Hemolysis assays
A female BALB/c mouse (22 g; age, 4 weeks) was anesthetized by the injection of pentobarbital sodium intraperitoneally at a dose of 50 mg/kg. The mouse was kept in a 20˚C environment with 40-60% humidity and a 12-h light/dark cycle and free access to food and water. Fresh mouse blood (0.1 ml) was collected from the orbit and centrifuged at 2,000 x g for 5 min (4˚C). The supernatant was discarded and the precipitated red blood cells were washed with PBS until the supernatant was colorless. The cells were then re-suspended in PBS to a concentration of 2% (w/v). Afterwards, the Pro/L and Pro/cRGDyK-L liposomes were diluted with PBS to prepare liposome samples with a range of lipid concentrations (10, 25, 50, 100, 200 and 400 nM). Subsequently, the liposome sample (0.4 ml) was mixed with the suspension of red blood cells (0.1 ml) and the mixture was maintained at 37˚C for 2 h with gentle shaking (50 rpm). After centrifuging at 6,000 x g for 10 min (4˚C), the absorbance of the supernatant at 540 nm (A540) was measured using a microplate reader. The hemolysis rate of each sample was calculated as follows: Hemolysis (%) = [(A540 sample - A540 negative) / (A540 positive - A540 negative)] x100. The absorbance of PBS and Triton X-100 mixed with the cell suspension were defined as 0 and 100%, respectively.
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay
A cytotoxicity assay was performed using a standard MTT-based colorimetric assay. In brief, bEnd.3 and C6 cells were bought from American Type Culture Collection and cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS at 37˚C in a humidified incubator containing 5% CO2. The cells were seeded into 96-well plates. In each well, cells at a density of ~5x103 cells/well were cultured for 24 h. Fresh medium with procaine, Pro/L and Pro/cRGDyK-L was applied in which the procaine concentration ranged from 0.01 to 10 mM. The cells were incubated for another 24 h at 37˚C, and then MTT (5 mg/ml) was added to each well with further culturing for another 4 h at 37˚C. Next, the cells were lysed using 200 µl DMSO and the absorbance at 490 nm (A490) was read using a microplate reader. Survival percentages were calculated using the following equation: Survival (%) = [(A490 treated cells) / (A490 control cells)] x100.
Transendothelial migration in an in vitro BBB model
Millicell® Hanging Cell Culture Inserts (Corning, Inc.) were used to build an in vitro BBB model as described in a previous study (25). Briefly, bEnd.3 cells were maintained in DMEM with 10% FBS and seeded on 6-well plate at a density of 1x106 cells/well and incubated for 7 days at 37˚C. The transendothelial electric resistance (TEER) of the BBB model was measured using a Millicell ERS volt-ohm meter (EMD Millipore). Only the bEnd.3 monolayers with a TEER >200 Ω were used for further experiments. In addition, C6 cells were plated on another 6-well plate. After transferring the cell culture inserts with bEnd.3 monolayers into the plates containing C6 cells, the cells were co-cultured for another 24 h at 37˚C. Subsequently, the CFPE-labeled liposomes (CFPE/L and CFPE/cRGDyK-L; final concentration, 2 µg/ml) were added to the cell culture inserts (donor chamber) of the BBB model and incubated for 4 h. After this, the bEnd.3 and C6 cells were each washed with cold PBS three times, trypsinized and resuspended in 0.5 ml PBS. The fluorescent intensity of the two types of cells was measured using a flow cytometer (Cytomics FC500; Beckman Coulter, Inc.) and corresponding software.
Wound healing assays
To determine the effects of the liposomal formulations on cell migration ability, the wound healing assay was performed. Briefly, C6 cells (confluence, 100%) treated with Pro/L or Pro/cRGDyK-L were wounded by scraping with a pipette tip, and the detached cells were washed away. Subsequently, the cells were cultured with serum-free DMEM (Gibco; Thermo Fisher Scientific, Inc.) to stimulate wound healing. Photographic images were captured using an Olympus light microscope at 0 h and 24 h later to evaluate the migration of the cells (cat. no. CKX53; Olympus Corporation). The images were then analyzed by ImageJ 8.0 software (National Institutes of Health).
Transwell assays
A total of 1x104 C6 cells treated with either Pro/L or Pro/cRGDyK-L were seeded in the upper chamber of Transwell plates in DMEM. The upper cells were induced to migrate toward the bottom chamber which contained DMEM plus 10% FBS at 37˚C for 24 h. Then, the cells were stained with 0.2% crystal violet for 20 min at 25˚C. Transwell migration was determined as the percentage of input cells minus the percentage of cells migrating to the medium alone (baseline). A light microscope (cat. no. IX71; Zeiss AG) was used for imaging at a magnification of x20. The images were then analyzed by ImageJ 8.0 software (National Institutes of Health).
Cell cycle assays
Flow cytometric analysis was performed to determine the cell cycle distribution of C6 cells. Following treatment with Pro/L or Pro/cRGDyK-L (1 mM calculated as Pro) at 37˚C for 24 h, the C6 glioma cells (4x105) were washed with PBS and fixed with pre-cooled 70% ethanol at -20˚C for 30 min. Following centrifugation (450 x g) at 4˚C for 5 min and washing with PBS, the cells were stained with 500 µl propidium iodide (PI; 50 µg/ml; Thermo Fisher Scientific, Inc.), followed by DNA content analyses using a flow cytometer (Cytomics FC500; Beckman Coulter, Inc.) and corresponding software. The percentage of cells in the G1, S and G2/M phases was calculated and analyzed.
Cell apoptosis assays
Cell apoptosis was evaluated using Annexin V-FITC and PI staining (Sangon Biotech Co., Ltd.). Following treatment of the C6 glioma cells with Pro/L or Pro/cRGDyK-L (0-10 mM calculated as Pro) at 37˚C for 48 h, the cells were centrifuged (200 x g) at 4˚C for 3 min and washed with PBS. Subsequently, the cells were re-suspended in 100 µl binding buffer with 5 µl Annexin V-FITC and incubated at room temperature for 10 min. Then, 5 µl PI solution was added and the cells were incubated for another 5 min at room temperature. The proportion of apoptotic cells was analyzed using a flow cytometer (Cytomics FC500; Beckman Coulter, Inc.) and corresponding software.
Immunoblot assay
Following treatment with Pro/L or Pro/cRGDyK-L (1 mM calculated as Pro) at 37˚C for 24 h, and protein determination was performed using the BCA method. Protein samples (10 µg/per lane) were separated on 10% SDS-PAGE gels and transferred onto PVDF membranes (250 mA, 2 h). The proteins were transferred onto PVDF membranes, which were then blocked with 5% milk in TBS with 0.05% Tween 20 at 25˚C for 2 h. The PVDF membranes were subsequently treated with primary antibodies targeting the following proteins: Phosphorylated (p-)ERK1/2 (ERK1 p-T202 + ERK2 p-T185; ab50011; 1:1,000 dilution; Abcam), ERK1/2 (ab17942; 1:1,000 dilution; Abcam), p-p38MAPK (1:500; cat. no. ab4822; Abcam), p38MAPK (1:500; cat. no. ab170099; Abcam) and β-actin (1:3,000; cat. no. ab8226; Abcam) at room temperature for 2 h. The membranes were then incubated with rabbit or mouse HRP-conjugated secondary antibodies (both 1:5,000; cat. nos. ab6271 and ab6728; both Abcam) at room temperature for 1 h. Protein signals were visualized via the use of an ECL kit (Novex™ ECL Chemiluminescent Substrate Reagent kit; Thermo Fisher Scientific, Inc.) and visualized by ImageJ 8.0 software (National Institutes of Health).
Antitumor activity in vivo
All animal experiments in this study, including the extraction of blood from mice in the hemolysis assay, were approved by the Laboratory Animal Ethics Committee of the Second Hospital of Tianjin Medical University. A total of 18 female BALB/c nude mice (4 weeks old; weight 18-20 g) were acquired from the Animal Core Facility. For the tumor growth assay, 1x107 C6 cells were mixed with Matrigel (BD Biosciences) in a 2:1 ratio and subcutaneously implanted into the nude mice to induce tumors for 7 days. To detect the in vivo antitumor effects of procaine and its liposomal formulations on glioma xenografts, the mice were divided into 3 groups (n=6/group), and procaine, Pro/L and Pro/cRGDyK-L were administered at a dose of 10 mg/kg, calculated as procaine, via intravenous injection every 3 days. After 12 days of treatment, all the mice were sacrificed by cervical dislocation, and the tumors were isolated from the mice and weighed.
Statistical analysis
All data are presented as the mean ± standard deviation (n=3). The statistical analyses were conducted using GraphPad Prism 6.0 (GraphPad Software, Inc.). Unpaired Student's t-test was used for statistical comparisons between two groups. Statistical comparisons were performed by one-way analysis of variance for multiple groups followed by Tukey's post hoc tests. P<0.05 was considered to indicate a statistically significant difference.
Results
Chemistry
The synthetic pathway of the cRGDyK-modified ligand is outlined in Fig. 1. Briefly, cholesterol was esterified with p-toluenesulfonyl chloride in pyridine, followed by etherification with octaethylene glycol. Then, a Williamson etherification reaction was conducted with tert-butyl bromoacetate in the mixed solvent of 50% NaOH/toluene. Subsequently, the tert-butyl ester was hydrolyzed in the presence of p-toluenesulfonic acid to obtain the free acid, which was then reacted with NHS/DCC to form an active ester. Finally, condensation of the peptide cRGDyK with the active ester produced the cRGDyK-modified ligand.
Characterization of liposomes
An appropriate size and uniform distribution are critical for procaine-loaded liposomes to cross the BBB and target glioma. The mean diameters and polydispersity index (PDI) were detected through dynamic light scattering, and the results indicated that the liposomes had a suitable size (107 or 114 nm) and PDI (<0.2). Furthermore, the data in Table I also show that the EE (%) of the liposomes was >85% and the DL (%) was ~2.8%. Also, the weak negative ζ potential (-7.91) of Pro/cRGDyK-L should decrease absorption by the reticuloendothelial system and the immune response.
An in vitro release assay of the liposomes was used to mimic drug release behavior in vivo and evaluate the entrapment, membrane flexibility and integrity, and affinity of the liposomal carrier systems. The results revealed that procaine from free procaine exhibited fast release, with ~75% procaine being released in 10 h (Fig. 2A). However, the two procaine-loaded liposomes released ~40% of the loaded procaine within 10 h, and the release rate was slow thereafter. Neither of the two liposomes exhibited an initial burst release pattern.
The stability of the liposomes Pro/L and Pro/cRGDyK-L under physiological conditions is critical for their application in vivo. In the stability assay, the transmittances of the two liposome formulations were >90%, with no marked variation between formulations during the 48-h culture in FBS (Fig. 2B). The results suggest that Pro/L and Pro/cRGDyK-L have adequate stability, which is important for further investigation in vivo. The hemocompatibility of Pro/L and Pro/cRGDyK-L was evaluated by a hemolysis assay, and the results indicated that the two liposome formulations did not induce an excessive increase in hemoglobin release at phospholipid concentrations of ≥400 nM (Fig. 2C), which implies that they have acceptable biosafety.
MTT assay
The cytotoxicity of the liposomes to bEnd.3 and C6 cells was evaluated using an MTT assay. As shown in Fig. 3A, free procaine exhibited a greater cytotoxic effect than the procaine-loaded liposomes on the C6 cells, because the free drugs could directly enter the cells by passive diffusion, without a drug-release process. In addition, Pro/cRGDyK-L had a stronger antiproliferative effect than Pro/L, presumably due to the ability of cRGDyK peptide to enhance cellular uptake. The inhibitory effects of the procaine formulations on bEnd.3 cells were also evaluated (Fig. 3B). The results revealed that the formulations had a weaker effect on the survival of the endothelial cells, which indicated the specific antitumor effects of procaine.
Transendothelial migration in a BBB model
The bEnd.3 cells were used to establish a BBB model in vitro. The bEnd.3 cells on the Transwell membrane in the donor chamber and the underlying layer of C6 cells on the plate from the acceptor chamber were collected, and the fluorescence intensity was measured using flow cytometry. The results revealed that the bEnd.3 monolayer on the Transwell membrane internalized liposomes by cellular uptake (Figs. 3C and S1). The CFPE/cRGDyK-L formulation induced a significant increase in the fluorescence intensity of the C6 cells after penetrating the bEnd.3 monolayer when compared with the CFPE/L formulation, which indicates that cRGDyK-L was transported across the bEnd.3 monolayer and delivered to the C6 cells. These results suggest that the transport of liposomes across the BBB model was enhanced when they were modified with cRGDyK peptide.
Wound closure and Transwell assays
Wound closure and Transwell assays were performed to investigate the effects of Pro/L and Pro/cRGDyK-L on C6 cell migration-. The results revealed that the Pro/cRGDyK-L formulation significantly suppressed the migration of C6 cells (Fig. 4A and B) in the wound healing assay compared with that in the control group. Similarly, the Transwell assay results confirmed that Pro/cRGDyK-L significantly inhibited the migration of C6 cells, with a stronger inhibitory effect than the Pro/L formulation (Fig. 4C and D). These results indicate that the suppressive effect of Pro/cRGDyK-L on glioma cell migration was superior to that of Pro/L.
Cell cycle and apoptosis detection assays
The effects of Pro/L and Pro/cRGDyK-L on the cell cycle and apoptosis of C6 glioma cells were investigated using flow cytometry. Notably, the results revealed that Pro/cRGDyK-L stimulated cell apoptosis in a concentration-dependent manner (Figs. 4E and S2). Interestingly, comparison with control cells revealed that Pro/L led to cell cycle arrest while treatment with Pro/cRGDyK-L resulted in increased cell cycle arrest at the G1 phase (Fig. 4F). These results indicate Pro/cRGDyK-L significantly stimulates glioma cell cycle arrest and apoptosis.
Pro/cRGDyK-L exhibits antitumor activity in glioma by targeting the ERK/p38MAPK pathway
The previous results demonstrate that Pro/cRGDyK-L can suppress the proliferation and migration of glioma cells and stimulate their apoptosis. By performing immunoblot assays, it was observed that the phosphorylation level of ERK was significantly decreased by treatment with Pro/cRGDyK-L and Pro/L, with Pro/cRGDyK-L exhibiting a stronger effect than Pro/L (Fig. 5A and B). Additionally, the results revealed that Pro/L treatment inhibited the phosphorylation of p38MAPK, and Pro/cRGDyK-L treatment decreased p38MAPK phosphorylation levels compared with those in the Pro/L treatment group (Fig. 5A and C). Therefore, it appears that Pro/cRGDyK-L exhibits antitumor activity in glioma by targeting the ERK/p38MAPK pathway.
The antitumor activity of Pro/cRGDyK-L was also confirmed in vivo. The findings from the xenograft assay demonstrate the effectiveness of this targeted therapy. The tumors observed in mice from the Pro/cRGDyK-L treatment group were smaller than those in the other two groups. Representative image of the tumors from each group are shown in Fig. 5D. Although there was no significant different in the weight of the mice in the three groups (Fig. 5E), the tumor volume (Fig. 5F) and weight (Fig. 5G) of the mice treated with Pro/cRGDyK-L were significantly reduced compared with those in the control group according to the tumor growth curves and tumor weight measurements, further demonstrating the antitumor effect of Pro/cRGDyK-L.
Discussion
Gliomas are the most common primary craniocerebral malignancies caused by cancerous changes in glial cells (26). The incidence rate of glioma among intracranial tumors is 35.2-61.0% (1). Notably, the incidence and recurrence rates of glioma are high, and the cure rate is low (27). However, due to the existence of the BBB, the invasion and metastasis of glioma is extremely low (27). However, existing therapeutic drugs are not adequate to meet clinical needs due to the BBB blocking their uptake into the brain (28). Therefore, it is urgently necessary to develop a novel strategy for targeted drug delivery to treat this particular malignancy.
Procaine is a type of local anesthetic (29). Procaine is used for local, regional and neuraxial anesthesia, and despite the emergence of several new anesthetic drugs, it remains a widely used anesthetic (30). Interestingly, procaine has also been revealed to have antitumor effects (31). A previous study indicated that procaine may serve as a specific DNA methylation inhibitor with antitumor effects in gastric cancer (31). Additionally, in other studies, procaine suppressed the proliferation and motility of colon cancer cells by suppressing the ERK/MAPK/FAK axis (32), and exhibited an inhibitory effect on osteosarcoma (33). These studies, together with the findings of the present study, indicate the potential role of procaine in tumor therapy.
In the present study, a cRGDyK-cholesterol derivative was designed and prepared, and used as a ligand in a liposomal drug delivery system. The procaine-loaded liposome Pro/cRGDyK-L was characterized for particle size, ζ potential, encapsulation efficiency, release profile, stability and hemolysis in vitro, and the results indicated the liposome had superior properties.
The present study also demonstrated that the Pro/cRGDyK-L formulation significantly enhanced the ability of procaine to penetrate the BBB and improve cellular uptake, and further revealed that it had strong effects on cell migration, induced apoptosis and led to cell cycle arrest. Notably, the present study also indicated that Pro/cRGDyK-L exhibited superior antitumor effects by targeting the ERK/p38MAPK pathway and inhibited tumor growth in vivo, as illustrated by the scheme in Fig. 6.
Tumor angiogenesis is an important factor that affects the prognosis of patients with tumors. During tumor growth, endothelial cells play a major role in the formation of new blood vessels that feed the tumor with nutrients (34). The results of the present study demonstrate that Pro/cRGDyK-L has an important effect on endothelial cells and thus may influence tumor angiogenesis and further affect tumor development.
In conclusion, with the aim of developing an efficient drug delivery system targeting glioma to improve the antitumor effect of procaine and explore its antitumor mechanism, a novel cRGDyK-cholesterol derivative was designed and synthesized for use in liposomes. The cyclic peptide markedly increase the ability of procaine to penetrate a model of the BBB and improved its cellular uptake. Additionally, the Pro/cRGDyK-L formulation exhibited strong inhibitory effects on cell migration, and also induced apoptosis and cell cycle arrest. The results further confirmed the superior antitumor effects of Pro/cRGDyK-L in vivo, which were mediated via targeting the ERK/p38MAPK pathway. Therefore, cRGDyK is indicated to be an effective ligand for BBB and glioma targeting, and the procaine-loaded liposome formulation Pro/cRGDyK-L exerts antitumor effects via targeting ERK/p38MAPK pathway and thereby suppresses tumor growth in mice.
Supplementary Material
Representative flow cytometry plots showing the uptake of CFPE-labeled liposomes by bEnd-3 and C6 cells. CFPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-carboxyfluorescein; L, conventional liposome; cRGDyk-L, cRGDyk-modified liposome.
Representative flow cytometry plots showing the apoptosis of C6 cells stimulated by Pro/L and Pro/cRGDyk-L at different procaine concentrations. Pro, procaine; L, conventional liposome; cRGDyk-L, cRGDyk-modified liposome.
Acknowledgements
Not applicable.
Funding
Funding: This study was supported by Science & Technology Development Fund of Tianjin Education Commission for Higher Education (grant no. 2020KJ174).
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Authors' contributions
DL, JG, CY and YW carried out the molecular biology experiments and drafted the manuscript. DL, JG, CY, BL, JS and MY participated in the design of the study and performed the statistical analysis. DL, HW and YL conceived the study, participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript. DL and YL confirm the authenticity of all the raw data.
Ethics approval and consent to participate
All procedures performed in the current study were approved by the Laboratory Animal Ethics Committee of the Second Hospital of Tianjin Medical University.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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