MicroRNA‑29b participates in the epithelial‑mesenchymal transition of retinal pigment epithelial cells through p‑p65

Li,Min; Li,Hui; Yang,Shuai; Liao,Xin; Zhao,Chun; Wang,Fang

doi:10.3892/etm.2021.10300

MicroRNA‑29b participates in the epithelial‑mesenchymal transition of retinal pigment epithelial cells through p‑p65

Authors:
- Min Li
- Hui Li
- Shuai Yang
- Xin Liao
- Chun Zhao
- Fang Wang
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Affiliations: Department of Ophthalmology, Shanghai Tenth People's Hospital, Shanghai 200072, P.R. China
Published online on: June 13, 2021 https://doi.org/10.3892/etm.2021.10300
Article Number: 868

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Abstract

Epithelial‑mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells is considered to be the main mechanism of proliferative vitreoretinopathy (PVR). Our previous study demonstrated that microRNA‑29b (miR‑29b) and its target protein kinase B (Akt2) played vital roles in this process. miR‑29b, a mesenchymal marker α‑smooth muscle actin (α‑SMA) and the epithelial marker E‑cadherin were assessed in epiretinal membranes of patients with PVR. The potential mechanism of miR‑29b and EMT was also investigated. The expression levels of miR‑29b, E‑cadherin, and α‑SMA in PVR epiretinal membranes were measured using quantitative PCR. The expression levels of Akt2, phosphorylated (p)‑Akt2, p65, p‑p65, and Snail in ARPE‑19 cells were assessed using western blotting. The expression levels of miR‑29b were positively correlated with E‑cadherin mRNA expression, while an inverse correlation was observed between miR‑29b and α‑SMA mRNA expression in epiretinal membranes of patients with PVR. When miR‑29b was transfected into ARPE‑19 cells, the expression levels of Akt2, p‑Akt2, p‑p65 and Snail were downregulated. shRNA‑Akt2 inhibited p‑p65 and Snail expression, while the NF‑κB inhibitor BAY11‑7082 reduced Snail expression. The Akt2/p‑p65/Snail pathway may be the underlying mechanism of miR‑29b in EMT of RPE cells. The results of the present study may provide a new strategy for prevention and therapy of PVR.

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