Insulin growth factor‑1 promotes the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells through the Wnt/β‑catenin pathway

Feng,Jing; Meng,Zhiqiang

doi:10.3892/etm.2021.10323

Insulin growth factor‑1 promotes the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells through the Wnt/β‑catenin pathway

Authors:
- Jing Feng
- Zhiqiang Meng
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Affiliations: Jiaozuo Coal Industry (Group) Co. Ltd. Central Hospital, Jiaozuo, Henan 454000, P.R. China, Department of Orthopedics, The General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China
Published online on: June 17, 2021 https://doi.org/10.3892/etm.2021.10323
Article Number: 891
Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Bone marrow mesenchymal stem cells (BMSCs) are stem cells that exist in bone marrow tissue and have osteogenic differentiation potential. Insulin growth factor‑1 (IGF‑1) plays a key role in the proliferation and osteogenic differentiation of BMSCs. However, the specific mechanism of IGF‑1 in cell proliferation and osteogenic differentiation remains unclear. In the present study, BMSCs were transfected with lentivirus carrying the siRNA‑Wnt3a gene, and the Wnt3a level in BMSCs was revealed to be reduced by western blotting, real‑time quantitative polymerase chain reaction and immunofluorescence detection. Then, BMSCs were treated with 80 ng/ml IGF‑1 in complete medium for 5 days. CCK‑8 and cell cycle assays revealed that cell proliferation was significantly decreased in the siRNA‑Wnt3a group than in the control group. The protein and mRNA levels of β‑catenin and cyclin D1 were significantly downregulated in the siRNA‑Wnt3a group compared with the control group. In addition, BMSCs were treated with IGF‑1 in osteogenic differentiation medium for 7 and 21 days, and alkaline phosphatase staining and Alizarin Red staining demonstrated significantly reduced osteogenic differentiation ability in the siRNA‑Wnt3a group compared with the control group. Furthermore, the protein and mRNA levels of β‑catenin, RUNX2, and OPN were downregulated compared with the control group. Our findings revealed that IGF‑1 promoted the proliferation and differentiation of BMSCs at least partially through the Wnt/β‑catenin pathway. These findings provided new insight into the clinical treatment of bone disease.

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