CGFe and TGF‑β1 enhance viability and osteogenic differentiation of human dental pulp stem cells through the MAPK pathway

Li,Xiaoju; Yang,Huixiao; Zhang,Yan; Du,Xinya; Yan,Zhengbin; Li,Jiang; Wu,Bin

doi:10.3892/etm.2021.10482

CGFe and TGF‑β1 enhance viability and osteogenic differentiation of human dental pulp stem cells through the MAPK pathway

Authors:
- Xiaoju Li
- Huixiao Yang
- Yan Zhang
- Xinya Du
- Zhengbin Yan
- Jiang Li
- Bin Wu
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Affiliations: Department of Stomatology, The People's Hospital of Longhua, Shenzhen, Guangdong 518109, P.R. China, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatological Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, P.R. China, Department of General Therapy Dentistry, Stomatology Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
Published online on: July 23, 2021 https://doi.org/10.3892/etm.2021.10482
Article Number: 1048
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to evaluate the effects of concentrated growth factor exudate (CGFe) and TGF‑β1 on the viability and osteogenic differentiation of human dental pulp stem cells (hDPSCs). CGFe was prepared from the peripheral blood of healthy donors (obtained with informed consent). STRO‑1+ hDPSCs were isolated from dental pulp tissues and treated in four groups: i) Control; ii) TGF‑β1 (1 ng/ml); iii) 100% CGFe; and iv) TGF‑β1 (1 ng/ml) + 100% CGFe group. hDPSC viability was measured via MTT assay. The osteogenic differentiation of hDPSCs was quantified via alkaline phosphatase (ALP) activity, western blotting and reverse transcription‑quantitative PCR assays. CGFe and TGF‑β1 enhanced hDPSC viability, upregulated ALP activity, upregulated the expression of phosphorylated (p)‑ERK1/2, p‑JNK and p‑p38 in hDPSCs, and promoted transcription and protein expression of osteogenic‑related genes (bone sialoprotein, Runt‑related transcription factor 2 and osteocalcin) in hDPSCs. The present study demonstrated that CGFe and TGF‑β1 facilitated the viability and osteogenic differentiation of hDPSCs potentially through activation of the MAPK signaling pathway.

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1	Chen FM and Yan J: Periodontal tissue engineering and regeneration: Current approaches and expanding opportunities. Tissue Eng Part B Rev. 16:219–255. 2010.PubMed/NCBI View Article : Google Scholar
2	Bosshardt DD and Sculean A: Does periodontal tissue regeneration really work? Periodontol. 5:208–219. 2009.PubMed/NCBI View Article : Google Scholar
3	Mey AH, Yu ZD and Huang TJ: Small molecules affect human dental pulp stem cell properties via multiple signaling pathways. Stem Cells Dev. 22:2402–2413. 2013.PubMed/NCBI View Article : Google Scholar
4	Yu J, Deng Z, Shi J, Zhai H, Nie X, Zhuang H, Li Y and Jin Y: Differentiation of dental pulp stem cells into regular-shaped dentin-pulp complex induced by tooth germ cell conditioned medium. Tissue Eng. 12:3097–3105. 2006.PubMed/NCBI View Article : Google Scholar
5	Wang Y, Li J, Song W and Yu J: Mineral trioxide aggregate upregulates odonto/osteogenic capacity of bone marrow stromal cells from craniofacial bones via JNK and ERK MAPK signalling pathways. Cell Prolif. 47:241–248. 2014.PubMed/NCBI View Article : Google Scholar
6	Zhang W, Shen X, Wan C, Zhao Q, Zhang L, Zhou Q and Deng L: Effects of insulin and insulin-like growth factor 1 on osteoblast proliferation and differentiation: Differential signalling via Akt and ERK. Cell Biochem Funct. 30:297–302. 2012.PubMed/NCBI View Article : Google Scholar
7	Kim TH, Kim SH, Sándor GK and Kim YD: Comparison of platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and concentrated growth factor (CGF) in rabbit-skull defect healing. Arch Oral Biol. 59:550–558. 2014.PubMed/NCBI View Article : Google Scholar
8	Park HC, Kim SG, Oh JS, You JS, Kim JS, Lim SC, Jeong MA, Kim JS, Jung C, Kwon YS and Ji H: Early bone formation at a femur defect using CGF and PRF grafts in adult dogs: A comparative study. Implant Dent. 25:387–393. 2016.PubMed/NCBI View Article : Google Scholar
9	Chin D, Boyle GM, Parsons PG and Coman WB: What is transforming growth factor-beta (TGF-beta)? Br J Plast Surg. 57:215–221. 2004.PubMed/NCBI View Article : Google Scholar
10	Kubiczkova L, Sedlarikova L, Hajek R and Sevcikova S: TGF-β- an excellent servant but a bad master. J Transl Med. 10(183)2012.PubMed/NCBI View Article : Google Scholar
11	Tang Y, Wu X, Lei W, Pang L, Wan C, Shi Z, Zhao L, Nagy TR, Peng X, Hu J, et al: TGF-beta1-induced migration of bone mesenchymal stem cells couples bone resorption with formation. Nat Med. 15:757–765. 2009.PubMed/NCBI View Article : Google Scholar
12	Vahabi S, Torshabi M and Nejad AE: In vitro comparison of the efficacy of TGF-β1 and PDGF-BB in combination with freeze-dried bone allografts for induction of osteogenic differention in MG-63 osteoblast-like cells. J Mater Sci Mater Med. 27(182)2016.PubMed/NCBI View Article : Google Scholar
13	Farea M, Husein A, Halim AS, Abdullah NA, Mokhtar KI, Lim CK, Berahim Z and Mokhtar K: Synergistic effects of chitosan scaffold and TGFβ1 on the proliferation and osteogenic differentiation of dental pulp stem cells derived from human exfoliated deciduous teeth. Arch Oral Biol. 59:1400–1411. 2014.PubMed/NCBI View Article : Google Scholar
14	Yu J, He H, Tang C, Zhang G, Li Y, Wang R, Shi J and Jin Y: Differentiation potential of STRO-1⁺ dental pulp stem cells changes during cell passaging. BMC Cell Biol. 11(32)2010.PubMed/NCBI View Article : Google Scholar
15	Li X, Yang H, Zhang Z, Yan Z, Lv H, Zhang Y and Wu B: Concentrated growth factor exudate enhances the proliferation of human periodontal ligament cells in the presence of TNF-α. Mol Med Rep. 19:943–950. 2019.PubMed/NCBI View Article : Google Scholar
16	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar
17	Qiao J, Duan J, Zhang Y, Chu Y and Sun C: The effect of concentrated growth factors in the treatment of periodontal intrabony defects. Future Sci OA. 2(FS136)2016.PubMed/NCBI View Article : Google Scholar
18	Rodella LF, Favero G, Boninsegna R, Buffoli B, Labanca M, Scarì G, Sacco L, Batani T and Rezzani R: Growth factors, CD34 positive cells, and fibrin network analysis in concentrated growth factors fraction. Microsc Res Tech. 74:772–777. 2011.PubMed/NCBI View Article : Google Scholar
19	Honda H, Tamai N, Naka N, Yoshikawa N and Myoui A: Bone tissue engineering with bone marrow-derived stromal cells integrated with concentrated growth factor in rattus norvegicus calvaria defect model. J Artif Organs. 16:305–315. 2013.PubMed/NCBI View Article : Google Scholar
20	Shinohara Y, Tsuchiya S, Hatae K and Honda MJ: Effect of vitronectin bound to insulin-like growth factor-I and insulin-like growth factor binding protein-3 on porcine enamel organ-derived epithelial cells. Int J Dent. 2012(386282)2012.PubMed/NCBI View Article : Google Scholar
21	Sarahrudi K, Thomas A, Mousavi M, Kaiser G, Köttstorfer J, Kecht M, Hajdu S and Aharinejad S: Elevated transforming growth factor-beta 1 (TGF-β1) levels in human fracture healing. Injury. 42:833–837. 2011.PubMed/NCBI View Article : Google Scholar
22	Poniatowski LA, Wojdasiewicz P, Gasik R and Szukiewicz D: . Transforming growth factor beta family: Insight into the role of growth factors in regulation of fracture healing biology and potential clinical application. Mediators Inflamm. 2015(137823)2015.PubMed/NCBI View Article : Google Scholar
23	Li F, Liu X, Zhao SL, Wu H and Xu HHK: Porous chitosan bilayer membrane containing TGF-β1 loaded microspheres for pulp capping and reparative dentin formation in a dog model. Dent Mater. 30:172–181. 2014.PubMed/NCBI View Article : Google Scholar
24	Hwang YC, Hwang IN, Oh WM, Park JC, Lee DS and Son HH: Influence of TGF-beta1 on the expression of BSP, DSP, TGF-beta1 receptor I and smad proteins during reparative dentinogenesis. J Mol Hist. 39:153–160. 2008.PubMed/NCBI View Article : Google Scholar
25	Chen Q, Liu WB, Sinha KM, Yasuda H and de Crombrugghe B: Identification and characterization of microRNAs controlled by the osteoblast-specific transcription factor osterix. PLoS One. 8(e58104)2013.PubMed/NCBI View Article : Google Scholar
26	Jeong W, Song G, Bazer FW and Kim J: Insulin-like growth factor I induces proliferation and migration of porcine trophectoderm cells through multiple cell signaling pathways, including protooncogenic protein kinase 1 and mitogen-activated protein kinase. Mol Cell Endocrinol. 384:175–184. 2014.PubMed/NCBI View Article : Google Scholar