Latent‑transforming growth factor β‑binding protein 2 accelerates cardiac fibroblast apoptosis by regulating the expression and activity of caspase‑3

Shi,Chunlai; Li,Xuan; Hong,Fei; Wang,Xueyan; Jiang,Tingting; Sun,Bingbing; Li,Shuang

doi:10.3892/etm.2021.10580

Latent‑transforming growth factor β‑binding protein 2 accelerates cardiac fibroblast apoptosis by regulating the expression and activity of caspase‑3

Authors:
- Chunlai Shi
- Xuan Li
- Fei Hong
- Xueyan Wang
- Tingting Jiang
- Bingbing Sun
- Shuang Li
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Affiliations: Cardiovascular Department, Jinqiu Hospital of Liaoning Province, Shenyang, Liaoning 110016, P.R. China
Published online on: August 9, 2021 https://doi.org/10.3892/etm.2021.10580
Article Number: 1146
Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cardiac fibrosis is a core process in the development of heart failure. However, the underlying mechanism of cardiac fibrosis remains unclear. Recently, a study found that in an isoproterenol (ISO)‑induced cardiac fibrosis animal model, there is high expression of latent‑transforming growth factor β‑binding protein 2 (LTBP2) in cardiac fibroblasts. Whether LTBP2 serves a role in cardiac fibrosis is currently unknown. In the present study, mouse cardiac fibroblasts (MCFs) were treated with 100 µM/l ISO for 24, 48, or 72 h, and small interfering RNAs (siRNAs) were used to knockdown LTBP2. Reverse transcription‑quantitative PCR and western blotting were used to determine gene and protein expression levels, respectively. Caspase‑3 serves a key role in cell apoptosis and is related to cardiac fibrosis‑induced heart failure. Caspase‑3 activity was therefore determined using a caspase‑3 assay kit, CCK8 was used to determine the rate of cell proliferation and apoptosis rates were quantified using a cell death detection ELISA kit. The present study demonstrated that cell apoptosis and LTBP2 expression increased in MCFs treated with 100 µM/l ISO in a time‑dependent manner. Expression and activity of caspase‑3 also increased in MCFs treated with 100 µM/l ISO for 48 h compared with the control group. In addition, ISO stimulation‑induced MCF apoptosis, along with the increased expression of caspase‑3 were partly abolished when LTBP2 was knocked down. In conclusion, LTBP2 expression increased in ISO‑treated MCFs and accelerated mouse cardiac fibroblast apoptosis by enhancing the expression and activity of caspase‑3. LTBP2 may therefore be a potential therapeutic target for treating patients with cardiac fibrosis.

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