Long non‑coding RNA SNHG1 relieves microglia activation by downregulating miR‑329‑3p expression in an <em>in vitro</em> model of cerebral infarction

He,Jianli; Xuan,Xianjun; Jiang,Minhai; Li,Jiangtao; Li,Ning; Nie,Tian

doi:10.3892/etm.2021.10581

Long non‑coding RNA SNHG1 relieves microglia activation by downregulating miR‑329‑3p expression in an in vitro model of cerebral infarction

Authors:
- Jianli He
- Xianjun Xuan
- Minhai Jiang
- Jiangtao Li
- Ning Li
- Tian Nie
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Affiliations: Department of Neurology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, P.R. China, The Fourth Clinical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310000, P.R. China, Department of Neurology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, P.R. China, Department of General Surgery, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, P.R. China, Department of Urology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, P.R. China
Published online on: August 9, 2021 https://doi.org/10.3892/etm.2021.10581
Article Number: 1148
Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Following cerebral infarction, activated microglia cells can release a large amount of inflammatory cytokines, thereby exacerbating neuronal damage. It has been demonstrated that the long non‑coding RNA small nucleolar RNA host gene 1 (SNHG1) exerts a protective effect against cerebral infarction. However, its specific role in cerebral infarction and underlying mechanism have yet to be fully elucidated. The present study aimed to investigate the effects of the SNHG1 and microRNA (miR)‑329‑3p in cerebral infarction and to determine the underlying molecular mechanisms. An in vitro oxygen‑glucose deprivation (OGD) model was established using the BV‑2 microglial cell line. The mRNA expression levels of SNHG1 and miR‑329‑3p were analyzed using reverse transcription‑quantitative PCR and the protein expression levels of cleaved caspase‑3 and caspase‑3 were detected using western blotting. The binding relationship between SNHG1 and miR‑329‑3p was predicted using starBase and verified using a dual luciferase reporter assay. The release of TNF‑α and nitric oxide, as well as caspase‑3 activity, were detected using appropriate commercial kits. Flow cytometry analysis was performed to measure cell apoptosis. The results of the present study revealed that the expression levels of SNHG1 were upregulated in the OGD‑induced BV‑2 cell model. miR‑329‑3p was discovered to directly target SNHG1, and its mRNA expression levels were downregulated in the OGD‑induced BV‑2 cell model. The SNHG1‑plasmid downregulated miR‑329‑3p expression levels, while this effect was reversed by transfection with the miR‑329‑3p mimic. The overexpression of SNHG1 or knockdown of miR‑329‑3p inhibited OGD‑induced BV‑2 cell activation. In conclusion, the results of the present study suggested that SNHG1 may reduce microglial cell activity by regulating the expression of miR‑329‑3p, indicating its potential protective role in cerebral infarction.

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