Knockdown of LINC01123 inhibits cell viability, migration and invasion via miR‑361‑3p/TSPAN1 targeting in cervical cancer

Li,Cui; Li,Yujuan; Zhang,Yuying; Yan,Hua; Huang,Chengxiang

doi:10.3892/etm.2021.10618

Knockdown of LINC01123 inhibits cell viability, migration and invasion via miR‑361‑3p/TSPAN1 targeting in cervical cancer

Authors:
- Cui Li
- Yujuan Li
- Yuying Zhang
- Hua Yan
- Chengxiang Huang
View Affiliations

Affiliations: Department of Gynecology, Linyi Central Hospital, Linyi, Shandong 276400, P.R. China
Published online on: August 16, 2021 https://doi.org/10.3892/etm.2021.10618
Article Number: 1184

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Cervical cancer (CC) is a type of gynecological malignancy that poses a significant threat to females. The aim of the present study was to examine the role of long intergenic non‑protein coding RNA 1123 (LINC01123) and its underlying molecular mechanism in the development of CC. mRNA expression levels of LINC01123 and microRNA (miR)‑361‑3p in CC tissue samples and cell lines were evaluated using reverse transcription‑quantitative PCR. Cell viability, migration and invasion were detected using 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide, wound healing and Transwell assays. Moreover, a xenograft tumor model was established for elucidating the influence of LINC01123 knockdown on tumor growth in vivo. A dual‑luciferase reporter assay was used to confirm the association between LINC01123 and miR‑361‑3p, and miR‑361‑3p and tetraspanin 1 (TSPAN1). Western blot analysis was used to determine TSPAN1 protein expression. LINC01123 expression was upregulated and miR‑361‑3p expression was reduced in CC tissue samples and cell lines. Knockdown of LINC01123 inhibited cell viability, migration and invasion in vitro, and suppressed tumor growth in vivo. Furthermore, LINC01123 targeted miR‑361‑3p and negatively regulated miR‑361‑3p expression. Overexpression of miR‑361‑3p inhibited cell viability, migration and invasion in HeLa and CaSki cells. Additionally, miR‑361‑3p targeted TSPAN1 and negatively regulated TSPAN1 expression. Inhibition of miR‑361‑3p and overexpression of TSPAN1 reversed the effect of LINC01123 knockdown on cell proliferation, migration and invasion in HeLa cells. Knockdown of LINC01123 inhibited cell proliferation, migration and invasion via miR‑361‑3p/TSPAN1 regulation in CC, which may present an effective target for treatment of CC.

View Figures

View References

1	Morris E and Roett MA: Genital cancers in Women: Cervical cancer. FP Essent. 438:18–23. 2015.PubMed/NCBI
2	Arbyn M, Weiderpass E, Bruni L, de Sanjosé S, Saraiya M, Ferlay J and Bray F: Estimates of incidence and mortality of cervical cancer in 2018: A worldwide analysis. Lancet Glob Health. 8:e191–e203. 2020.PubMed/NCBI View Article : Google Scholar
3	Kori M and Yalcin Arga K: Potential biomarkers and therapeutic targets in cervical cancer: Insights from the meta-analysis of transcriptomics data within network biomedicine perspective. PLoS One. 13(e0200717)2018.PubMed/NCBI View Article : Google Scholar
4	Li H, Wu X and Cheng X: Advances in diagnosis and treatment of metastatic cervical cancer. J Gynecol Oncol. 27(e43)2016.PubMed/NCBI View Article : Google Scholar
5	Yee GP, de Souza P and Khachigian LM: Current and potential treatments for cervical cancer. Curr Cancer Drug Targets. 13:205–220. 2013.PubMed/NCBI View Article : Google Scholar
6	Sanchez Calle A, Kawamura Y, Yamamoto Y, Takeshita F and Ochiya T: Emerging roles of long non-coding RNA in cancer. Cancer Sci. 109:2093–2100. 2018.PubMed/NCBI View Article : Google Scholar
7	Luan X and Wang Y: LncRNA XLOC_006390 facilitates cervical cancer tumorigenesis and metastasis as a ceRNA against miR-331-3p and miR-338-3p. J Gynecol Oncol. 29(e95)2018.PubMed/NCBI View Article : Google Scholar
8	Liu Y, Yang Y, Li L, Liu Y, Geng P, Li G and Song H: LncRNA SNHG1 enhances cell proliferation, migration, and invasion in cervical cancer. Biochem Cell Biol. 96:38–43. 2018.PubMed/NCBI View Article : Google Scholar
9	Ye S, Sun B, Wu W, Yu C, Tian T, Lian Z, Liang Q and Zhou Y: LINC01123 facilitates proliferation, invasion and chemoresistance of colon cancer cells. Biosci Rep. 40(BSR20194062)2020.PubMed/NCBI View Article : Google Scholar
10	Hua Q, Jin M, Mi B, Xu F, Li T, Zhao L, Liu J and Huang G: LINC01123, a c-Myc-activated long non-coding RNA, promotes proliferation and aerobic glycolysis of non-small cell lung cancer through miR-199a-5p/c-Myc axis. J Hematol Oncol. 12(91)2019.PubMed/NCBI View Article : Google Scholar
11	Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004.PubMed/NCBI View Article : Google Scholar
12	Juan C, Hua Q, Ruping Z and Tingting W: miRNA-489 as a biomarker in diagnosis and treatment of cervical cancer. Bratisl Lek Listy. 119:278–283. 2018.PubMed/NCBI View Article : Google Scholar
13	Yin XZ, Zhao DM, Zhang GX and Liu L: Effect of miRNA-203 on cervical cancer cells and its underlying mechanism. Genet Mol Res. 15:2016.PubMed/NCBI View Article : Google Scholar
14	Hua FF, Liu SS, Zhu LH, Wang YH, Liang X, Ma N and Shi HR: MiRNA-338-3p regulates cervical cancer cells proliferation by targeting MACC1 through MAPK signaling pathway. Eur Rev Med Pharmacol Sci. 21:5342–5352. 2017.PubMed/NCBI View Article : Google Scholar
15	Liu S, Song L, Yao H, Zhang L, Xu D, Li Q and Li Y: Preserved miR-361-3p Expression is an independent prognostic indicator of favorable survival in cervical cancer. Dis Markers. 2018(8949606)2018.PubMed/NCBI View Article : Google Scholar
16	Wang J, Li H and Liang Z: circ-MYBL2 serves as a sponge for miR-361-3p promoting cervical cancer cells proliferation and invasion. Onco Targets Ther. 12:9957–9964. 2019.PubMed/NCBI View Article : Google Scholar
17	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar
18	Matsuo K, Machida H, Mandelbaum RS, Konishi I and Mikami M: Validation of the 2018 FIGO cervical cancer staging system. Gynecol Oncol. 152:97–93. 2019.PubMed/NCBI View Article : Google Scholar
19	Tewari KS, Sill MW, Long HJ III, Penson RT, Huang H, Ramondetta LM, Landrum LM, Oaknin A, Reid TJ, Leitao MM, et al: Improved survival with bevacizumab in advanced cervical cancer. N Engl J Med. 370:734–743. 2014.PubMed/NCBI View Article : Google Scholar
20	Hsu W, Liu L, Chen X, Zhang Y and Zhu W: LncRNA CASC11 promotes the cervical cancer progression by activating Wnt/beta-catenin signaling pathway. Biol Res. 52(33)2019.PubMed/NCBI View Article : Google Scholar
21	Wang R, Li Y, Du P, Zhang X, Li X and Cheng G: Hypomethylation of the lncRNA SOX21-AS1 has clinical prognostic value in cervical cancer. Life Sci. 233(116708)2019.PubMed/NCBI View Article : Google Scholar
22	Zhang P, Long Q, Zeng S, Wen M and Lu Q: FOXC1-induced LINC01123 acts as a mediator in triple negative breast cancer. Cancer Cell Int. 20(199)2020.PubMed/NCBI View Article : Google Scholar
23	Yang Y, Wu J, Zhou H, Liu W, Wang J and Zhang Q: STAT1-induced upregulation of lncRNA LINC01123 predicts poor prognosis and promotes the progression of endometrial cancer through miR-516b/KIF4A. Cell Cycle. 19:1502–1516. 2020.PubMed/NCBI View Article : Google Scholar
24	Liu J, Zhu J, Xiao Z, Wang X and Luo J: BBOX1-AS1 contributes to colorectal cancer progression by sponging hsa-miR-361-3p and targeting SH2B1. FEBS Open Bio, Jan 27, 2020 (Epub ahead of print).
25	Xia F, Chen Y, Jiang B, Bai N and Li X: Hsa_circ_0011385 accelerates the progression of thyroid cancer by targeting miR-361-3p. Cancer Cell Int. 20(49)2020.PubMed/NCBI View Article : Google Scholar
26	Tay Y, Rinn J and Pandolfi PP: The multilayered complexity of ceRNA crosstalk and competition. Nature. 505:344–352. 2014.PubMed/NCBI View Article : Google Scholar
27	Shang T, Zhou X and Chen W: LINC01123 promotes progression of colorectal cancer via miR-625-5p/LASP1 axis. Cancer Biother Radiopharm, May 18, 2020 (Epub ahead of print).
28	Li F, Zhu W and Wang Z: Long noncoding RNA LINC00460 promotes the progression of cervical cancer via regulation of the miR-361-3p/Gli1 axis. Hum Cell. 34:229–237. 2021.PubMed/NCBI View Article : Google Scholar
29	Chen W, Wang J, Liu S, Wang S, Cheng Y, Zhou W, Duan C and Zhang C: MicroRNA-361-3p suppresses tumor cell proliferation and metastasis by directly targeting SH2B1 in NSCLC. J Exp Clin Cancer Res. 35(76)2016.PubMed/NCBI View Article : Google Scholar
30	Huang S, Yuan S, Dong M, Su J, Yu C, Shen Y, Xie X, Yu Y, Yu X, Chen S, et al: The phylogenetic analysis of tetraspanins projects the evolution of cell-cell interactions from unicellular to multicellular organisms. Genomics. 86:674–684. 2005.PubMed/NCBI View Article : Google Scholar
31	Duan J, Liu J, Liu Y, Huang B and Rao L: miR-491-3p suppresses the growth and invasion of osteosarcoma cells by targeting TSPAN1. Mol Med Rep. 16:5568–5574. 2017.PubMed/NCBI View Article : Google Scholar
32	Wang S, Liu X, Khan AA, Li H, Tahir M, Yan X, Wang J and Huang H: miR-216a-mediated upregulation of TSPAN1 contributes to pancreatic cancer progression via transcriptional regulation of ITGA2. Am J Cancer Res. 10:1115–1129. 2020.PubMed/NCBI
33	Chen L, Li X, Wang GL, Wang Y, Zhu YY and Zhu J: Clinicopathological significance of overexpression of TSPAN1, Ki67 and CD34 in gastric carcinoma. Tumori. 94:531–538. 2008.PubMed/NCBI
34	Scholz CJ, Kurzeder C, Koretz K, Windisch J, Kreienberg R, Sauer G and Deissler H: Tspan-1 is a tetraspanin preferentially expressed by mucinous and endometrioid subtypes of human ovarian carcinomas. Cancer Lett. 275:198–203. 2009.PubMed/NCBI View Article : Google Scholar
35	Tian J, Zhang R, Piao H, Li X, Sheng W, Zhou J, Dong M, Zhang X, Yan X, Shang W, et al: Silencing Tspan1 inhibits migration and invasion, and induces the apoptosis of human pancreatic cancer cells. Mol Med Rep. 18:3280–3288. 2018.PubMed/NCBI View Article : Google Scholar
36	Hölters S, Anacker J, Jansen L, Beer-Grondke K, Dürst M and Rubio I: Tetraspanin 1 promotes invasiveness of cervical cancer cells. Int J Oncol. 43:503–512. 2013.PubMed/NCBI View Article : Google Scholar
37	Lu Z, Luo T, Nie M, Pang T, Zhang X, Shen X, Ma L, Bi J, Wei G, Fang G and Xue X: TSPAN1 functions as an oncogene in gastric cancer and is downregulated by miR-573. FEBS Lett. 589:1988–1994. 2015.PubMed/NCBI View Article : Google Scholar