TFAP4 promotes the growth of prostate cancer cells by upregulating FOXK1 Retraction in /10.3892/etm.2024.12754

Gu,Yuan; Jiang,Jiujin; Liang,Chaozhao

doi:10.3892/etm.2021.10734

TFAP4 promotes the growth of prostate cancer cells by upregulating FOXK1

Retraction in: /10.3892/etm.2024.12754

Authors:
- Yuan Gu
- Jiujin Jiang
- Chaozhao Liang
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Affiliations: Department of Urology, Anhui No. 2 Provincial People's Hospital, Hefei, Anhui 230041, P.R. China, Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230041, P.R. China
Published online on: September 16, 2021 https://doi.org/10.3892/etm.2021.10734
Article Number: 1299
Copyright: © Gu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Transcription factor activating enhancer binding protein 4 (TFAP4) has been indicated to be correlated with the progression of various human malignancies. However, the effect and regulatory mechanism of TFAP4 in prostate cancer (PC) remain unclear. The protein and mRNA expression were detected by western blotting and RT‑qPCR. TFAP4 was overexpressed or knocked down in PC cells. The viability, invasion and migration of PC cells were analyzed by CCK‑8, Transwell and wound healing assays. The colony formation was also determined. TFAP4 expression was upregulated in PC patients and cells; high TFAP4 expression predicted poor prognosis, and was associated with a range of clinicopathological features, including metastasis, clinical stage and Gleason score. Moreover, overexpression of TFAP4 promoted cell viability, migration, and invasion in vitro, whereas knockdown of TFAP4 revealed the opposite results. TFAP4 also positively regulated forkhead box K1 (FOXK1) expression. In addition, overexpression of FOXK1 reversed the effects of TFAP4 knockdown on PC cells. These findings clarified the biologic significance of TFAP4 in PC progression and revealed an association between TFAP4 and FOXK1, thus providing a new potential target for clinical therapy of PC.

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