Reducing oxidative stress may be important for treating pirarubicin‑induced cardiotoxicity with schisandrin B

Tang,Heng; Zhao,Junhao; Feng,Rui; Pu,Peng; Wen,Li

doi:10.3892/etm.2021.10991

Reducing oxidative stress may be important for treating pirarubicin‑induced cardiotoxicity with schisandrin B

Authors:
- Heng Tang
- Junhao Zhao
- Rui Feng
- Peng Pu
- Li Wen
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Affiliations: Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400042, P.R. China, The First Clinical College, Jinyun Mountain Campus of Chongqing Medical University, Chongqing 401331, P.R. China
Published online on: November 23, 2021 https://doi.org/10.3892/etm.2021.10991
Article Number: 68
Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The cardiotoxicity of pirarubicin (THP) seriously affects its clinical application, which cannot be ignored. The antioxidant effect of schisandrin B (SchB) has been extensively reported in the context of dietotherapy. However, whether this antioxidant effect can protect the heart from THP damage remains unknown. The aim of the present study was to investigate whether the antioxidant effect of SchB can antagonize the cardiotoxicity of THP. Changes in electrocardiogram (ECG), echocardiography and serum lactate dehydrogenase, brain natriuretic peptide, creatine kinase MB and cardiac troponin T levels were used to detect the degree of cardiac damage. The levels of superoxide dismutase (SOD), malondialdehyde, catalase and total antioxidant capacity in the serum and heart were measured to observe the oxidative stress state of rats. Primary cardiomyocytes were cultured, and cell viability and reactive oxygen species (ROS) production were detected. Western blotting was used to detect the expression levels of SOD2, NOX2, pro/cleaved‑caspase3 and Bcl‑2/Bax in heart tissue and primary cardiomyocytes to verify the related signaling pathways. THP‑treated rats showed a range of cardiac damage, including an abnormal ECG, echocardiography and myocardial enzymes. In the cellular experiments, cell viability decreased and ROS increased. However, this damage was alleviated after SchB treatment. Further studies demonstrated that SchB antagonized THP cardiotoxicity via its antioxidant effect. In conclusion, SchB protects the heart from THP damage in rats, and the mechanism may be closely associated with its antioxidant effect.

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