Open Access

Effects of Streptococcus thermophilus on anterior cruciate ligament transection-induced early osteoarthritis in rats

  • Authors:
    • Yen-You Lin
    • Nan-Fu Chen
    • San-Nan Yang
    • Yen-Hsuan Jean
    • Hsiao-Mei Kuo
    • Pei-Chin Chen
    • Chien-Wei Feng
    • Yu-Wei Liu
    • Yu-Cheng Lai
    • Zhi-Hong Wen
  • View Affiliations

  • Published online on: January 18, 2021     https://doi.org/10.3892/etm.2021.9653
  • Article Number: 222
  • Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteoarthritis (OA) is the most common joint disorder and is classically defined as a progressively degenerative disease of articular cartilage. It manifests as joint pain and disability and currently has no comprehensive treatments. The primary purpose of the present study was to test the effects of probiotics, Streptococcus thermophilus (TCI633), on anterior cruciate ligament transection (ACLT)‑induced experimental osteoarthritis (OA) in rats. In the current study, the experimental groups were given TCI633 (5x109, 5x1010 and 5x1011 CFU/kg/day) and glucosamine sulfate (250 mg/kg) between week 8 and 20 following ACLT. The results showed that oral administration of TCI633 and glucosamine had significant therapeutic effects on pain behaviors and knee swelling. Dose‑dependent effects of TCI633 were also observed in ACLT‑treated rats. Histopathological analysis demonstrated that ACLT+TCI633 (5x109, 5x1010 and 5x1011 CFU/kg/day) improved the synovial inflammation and cartilage damage of ACLT rats. Histology evaluation using the Osteoarthritis Research Society International system and synovial inflammatory score analysis showed the dose‑dependent inhibition of TCI633 on synovial inflammation and cartilage damage. Immunohistochemical staining and TUNEL apoptosis staining showed that TCI633 could effectively increase the expression of type II collagen and reduce the amount of chondrocyte apoptosis in cartilage. Therefore, the present study demonstrated that oral intake of TCI633 could significantly suppressing pain behavior, reduce joint swelling and synovial tissue inflammation and increase type II collagen expression in cartilage. There was also a reduction in chondrocyte apoptosis and decreased progression of OA in ACLT‑treated rats.
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March-2021
Volume 21 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Lin Y, Chen N, Yang S, Jean Y, Kuo H, Chen P, Feng C, Liu Y, Lai Y, Wen Z, Wen Z, et al: Effects of <em>Streptococcus thermophilus</em> on anterior cruciate ligament transection-induced early osteoarthritis in rats. Exp Ther Med 21: 222, 2021.
APA
Lin, Y., Chen, N., Yang, S., Jean, Y., Kuo, H., Chen, P. ... Wen, Z. (2021). Effects of <em>Streptococcus thermophilus</em> on anterior cruciate ligament transection-induced early osteoarthritis in rats. Experimental and Therapeutic Medicine, 21, 222. https://doi.org/10.3892/etm.2021.9653
MLA
Lin, Y., Chen, N., Yang, S., Jean, Y., Kuo, H., Chen, P., Feng, C., Liu, Y., Lai, Y., Wen, Z."Effects of <em>Streptococcus thermophilus</em> on anterior cruciate ligament transection-induced early osteoarthritis in rats". Experimental and Therapeutic Medicine 21.3 (2021): 222.
Chicago
Lin, Y., Chen, N., Yang, S., Jean, Y., Kuo, H., Chen, P., Feng, C., Liu, Y., Lai, Y., Wen, Z."Effects of <em>Streptococcus thermophilus</em> on anterior cruciate ligament transection-induced early osteoarthritis in rats". Experimental and Therapeutic Medicine 21, no. 3 (2021): 222. https://doi.org/10.3892/etm.2021.9653