miR‑146b correlates with increased disease activity and psoriatic tissue inflammation and promotes keratinocyte proliferation in psoriasis

Zhang,Ling; Zhang,Shenglan; Wang,Jianbin; Li,Xiaojing

doi:10.3892/etm.2021.9727

miR‑146b correlates with increased disease activity and psoriatic tissue inflammation and promotes keratinocyte proliferation in psoriasis

Authors:
- Ling Zhang
- Shenglan Zhang
- Jianbin Wang
- Xiaojing Li
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Affiliations: Department of Dermatology, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei 056002, P.R. China, Medical Department, Handan Central Hospital, Handan, Hebei 056002, P.R. China
Published online on: January 28, 2021 https://doi.org/10.3892/etm.2021.9727
Article Number: 296
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the expression of microRNA (miR)‑146b in psoriatic tissue and its correlation with psoriasis activity and inflammation. The effect of miR‑146b overexpression on keratinocyte proliferation and apoptosis was also explored. The expression of miR‑146b in the psoriasis‑affected tissue and non‑affected tissue of 110 patients was determined via reverse transcription‑quantitative (RT‑q)PCR. The psoriasis‑affected body surface area and psoriasis area severity index (PASI) score were recorded for evaluating disease activity. The expression of various inflammatory cytokines in psoriasis‑affected tissue was also detected via RT‑qPCR. miR‑146b overexpression and control plasmids were constructed and transfected into HaCaT cells in vitro. Subsequently, cell proliferation, apoptosis and tumor necrosis factor (TNF)‑related apoptosis‑inducing ligand (TRAIL)‑induced cell apoptosis were determined. The results revealed that the expression of miR‑146b was increased in psoriasis‑affected tissue compared with that in unaffected tissue. The results obtained from a receiver operating characteristic curve analysis demonstrated that miR‑146b levels were able to discriminate between psoriasis‑affected tissue and unaffected tissue, with an area under the curve value of 0.781 (95% CI: 0.720‑0.843). In addition, miR‑146b expression in psoriatic tissue was correlated with an increased PASI score in patients with psoriasis. miR‑146b expression in psoriatic tissue was positively correlated with TNF‑α, interleukin (IL)‑6 and IL‑17 mRNA levels. In vitro, miR‑146b overexpression enhanced HaCaT cell proliferation and suppressed apoptosis as well as TRAIL‑induced apoptosis when compared with that in control‑transfected HaCaT cells. In conclusion, miR‑146b was positively correlated with disease activity and psoriatic tissue inflammation. Keratinocyte proliferation was also promoted in psoriasis.

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