miR‑454‑3p prevents ox‑LDL‑induced apoptosis in HAECs by targeting TRPC3

Liao,Luming; Yang,Qiaolan; Li,Hailiang; Meng,Rongsen; Li,Yuntian

doi:10.3892/etm.2021.9754

miR‑454‑3p prevents ox‑LDL‑induced apoptosis in HAECs by targeting TRPC3

Authors:
- Luming Liao
- Qiaolan Yang
- Hailiang Li
- Rongsen Meng
- Yuntian Li
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Affiliations: The Second School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China, Department of General Surgery, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong 510317, P.R. China, Department of Cardiovascular Internal Medicine, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong 510317, P.R. China
Published online on: February 5, 2021 https://doi.org/10.3892/etm.2021.9754
Article Number: 323
Copyright: © Liao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Endothelial‑cell (EC) apoptosis serves a vital role in the pathogenesis of atherosclerosis. Accumulating evidence has implicated microRNA (miRNA/miR) dysregulation in EC apoptosis. Although the role of miR‑454‑3p in carcinogenesis has been well documented, its role and underlying mechanism in EC apoptosis remain unclear. In the present study, the results revealed that miR‑454‑3p expression was substantially downregulated in human aortic endothelial cells (HAECs) following oxidized low‑density lipoprotein (ox‑LDL) treatment. miR‑454‑3p suppression significantly attenuated the viability of HAECs, while miR‑454‑3p overexpression repressed ox‑LDL‑induced HAEC apoptosis. Bioinformatics analysis and luciferase reporter assays revealed that transient receptor potential canonical 3 (TRPC3), a key regulator of atherosclerosis development, was the direct target of miR‑454‑3p. Furthermore, TRPC3 overexpression abolished the anti‑apoptotic effect of miR‑454‑3p on HAECs. These results revealed a novel role of miR‑454‑3p in ox‑LDL‑induced apoptosis in HAECs.

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