Open Access

Combination of baicalein and miR‑106a‑5p mimics significantly alleviates IL‑1β‑induced inflammatory injury in CHON‑001 cells

  • Authors:
    • Qingtian Xiang
    • Jijun Wang
    • Tongwei Wang
    • Hongguang Zuo
  • View Affiliations

  • Published online on: February 11, 2021     https://doi.org/10.3892/etm.2021.9776
  • Article Number: 345
  • Copyright: © Xiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Osteoarthritis (OA) induces inflammation and degeneration of all joint components, and as such, is a considerable source of disability, pain and socioeconomic burden worldwide. Baicalein (BAI) and microRNA (miR)‑106a‑5p suppress the progression of OA; however, the effects of BAI and miR‑106a‑5p for the combined treatment of OA are not completely understood. An in vitro OA model was established by treating CHON‑001 cells with 20 ng/ml interleukin (IL)‑1β. Cell Counting Kit‑8 and flow cytometry assays were conducted to evaluate cell viability and apoptosis, respectively. Western blotting was performed to determine the expression levels of Bax, active caspase‑3, Bcl‑2, collagen I, collagen III, aggrecan, matrix metallopeptidase (MMP)‑13, MMP‑9, active Notch1 and transcription factor hes family bHLH transcription factor 1 (Hes1). The levels of IL‑6 and tumor necrosis factor‑α in the cell culture medium were quantified via ELISA. The present study revealed that treatment with BAI or miR‑106a‑5p mimic alleviated IL‑1β‑induced apoptosis, and BAI + miR‑106a‑5p combination treatment exerted enhanced anti‑inflammatory effects compared with monotherapy. Furthermore, IL‑1β‑induced accumulation of collagen, collagen III, MMP‑13 and MMP‑9 in CHON‑001 cells was reversed to a greater degree following combination treatment compared with monotherapy. Likewise, IL‑1β‑induced aggrecan degradation was markedly reversed by combination treatment. IL‑1β‑induced upregulation of active Notch1 and Hes1 in CHON‑001 cells was also significantly attenuated by combined BAI + miR‑106a‑5p treatment. In conclusion, the results of the present study revealed that the combination of BAI and miR‑106a‑5p mimic significantly decreased IL‑1β‑induced inflammatory injury in CHON‑001 cells, which may serve as a novel therapeutic strategy for OA.
View Figures
View References

Related Articles

Journal Cover

April-2021
Volume 21 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Xiang Q, Wang J, Wang T and Zuo H: Combination of baicalein and miR‑106a‑5p mimics significantly alleviates IL‑1β‑induced inflammatory injury in CHON‑001 cells. Exp Ther Med 21: 345, 2021
APA
Xiang, Q., Wang, J., Wang, T., & Zuo, H. (2021). Combination of baicalein and miR‑106a‑5p mimics significantly alleviates IL‑1β‑induced inflammatory injury in CHON‑001 cells. Experimental and Therapeutic Medicine, 21, 345. https://doi.org/10.3892/etm.2021.9776
MLA
Xiang, Q., Wang, J., Wang, T., Zuo, H."Combination of baicalein and miR‑106a‑5p mimics significantly alleviates IL‑1β‑induced inflammatory injury in CHON‑001 cells". Experimental and Therapeutic Medicine 21.4 (2021): 345.
Chicago
Xiang, Q., Wang, J., Wang, T., Zuo, H."Combination of baicalein and miR‑106a‑5p mimics significantly alleviates IL‑1β‑induced inflammatory injury in CHON‑001 cells". Experimental and Therapeutic Medicine 21, no. 4 (2021): 345. https://doi.org/10.3892/etm.2021.9776