MSK1 downregulation is involved in inflammatory responses following subarachnoid hemorrhage in rats

Ning,Bo; Li,Zhen; Ning,Lei; Wu,Jun; Chen,Xin; Jiang,Pengjun; Lin,Fuxin; Zhao,Bing

doi:10.3892/etm.2021.9795

MSK1 downregulation is involved in inflammatory responses following subarachnoid hemorrhage in rats

Authors:
- Bo Ning
- Zhen Li
- Lei Ning
- Jun Wu
- Xin Chen
- Pengjun Jiang
- Fuxin Lin
- Bing Zhao
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Affiliations: Department of Neurosurgery, Shandong Provincial Hospital, Shandong University, Jinan, Shandong 250021, P.R. China, Department of Medical Records, Affiliated Hospital of Taishan Medical University, Taishan Medical University, Tai'an, Shandong 271000, P.R. China, Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China, Department of Neurosurgery, The First Affiliated Hospital of Fujian Medical University, Fujian Medical University, Fuzhou, Fujian 350005, P.R. China, Department of Neurosurgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China
Published online on: February 18, 2021 https://doi.org/10.3892/etm.2021.9795
Article Number: 364
Copyright: © Ning et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Subarachnoid hemorrhage (SAH) is a life‑threatening neurological disease. Recently, inflammatory factors have been confirmed to be responsible for the brain damage associated with SAH. Therefore, studying the post‑SAH inflammatory reaction may clarify the mechanism of SAH. Mitogen and stress‑activated protein kinase 1 (MSK1) causes the phosphorylation of NF‑κB and regulates the activity of pro‑inflammatory transcription factors. The present study aimed to identify the potential role of MSK1 in inflammation and brain damage development following SAH. A cisterna magna blood injection model was established in Sprague‑Dawley rats. Hematoxylin and eosin staining, reverse transcription‑quantitative polymerase chain reaction assays and double immunofluorescence staining were used to analyze the role of MSK1, IL‑1β and TNF‑α in the inflammatory process after SAH. In a model of lipopolysaccharide‑induced astrocyte inflammation, the effect of overexpressing MSK1 overexpression was analyzed by western blot analysis. The results demonstrated that MSK1 expression were negatively correlated with TNF‑α and IL‑1β expression levels, and reached peak levels 2 days after TNF‑α and IL‑1β. The double immunofluorescence staining results showed that the expression of MSK1 was in the same plane of view as TNF‑α and IL‑1β in the brain cortex. Furthermore, the in vitro studies indicated that the overexpression of MSK1 inhibited the expression of TNF‑α and IL‑1β following LPS challenge. These results imply that MSK1 may be involved in the inflammatory reaction following SAH, and may potentially serve as a negative regulator of inflammation.

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