search button
Open Access

Visfatin promotes angiogenesis of RF/6A cells through upregulation of VEGF/VEGFR‑2 under high‑glucose conditions

  • Authors:
    • Dongjun Chen
    • Yi Wang
    • Mengyi Liu
    • Jing Cheng
    • Zhe Liu
    • Yang Song
    • Junhui Du

  • View Affiliations

  • Published online on: February 24, 2021     https://doi.org/10.3892/etm.2021.9820
  • Article Number: 389

  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Visfatin is a type of adipocytokine that is highly expressed in the serum and vitreous of patients with diabetic retinopathy. The purpose of the present study was to investigate the effect and mechanism of visfatin on angiogenesis in RF/6A monkey chorioretinal retinal endothelial cells under high glucose (HG) conditions in vitro. RF/6A cells were randomly divided into four groups: Control group, under high glucose (HG) group (25 mM D‑glucose), visfatin group 1 (10 nM visfatin + 25 mM D‑glucose), visfatin group 2 (20 nM visfatin + 25 mM D‑glucose) and visfatin group 3 (30 nM visfatin + 25 mM D‑glucose). After 24 and 48 h, a Cell Counting Kit‑8, wound‑healing assay and Matrigel tube formation assay were used to detect cell proliferation, migration and cell tube formation, respectively. Subsequently, the expression levels of VEGF and VEGF receptor 2 (VEGFR‑2) in cells of visfatin group 3 were observed by western blot and reverse transcription‑quantitative PCR analyses. At 24 and 48 h, the cell proliferation and migration distance in the HG group were reduced compared with those in the control group (P<0.05). Compared with those in the HG group, the cell proliferation and migration distance in all visfatin groups were significantly increased (P<0.05), with the highest significance in visfatin group 3. Visfatin significantly promoted tube‑like structure formation by RF/6A cells, particularly at the concentration of 30 nM. The protein and mRNA expression levels of VEGF and VEGFR‑2 were significantly increased in the HG group as compared with those in the control group (P<0.05). Furthermore, compared with those in the HG group, VEGF and VEGFR‑2 protein and mRNA expression levels were significantly increased in visfatin group 3 (P<0.05). Overall, visfatin promoted the proliferation, migration and tube formation of RF/6A cells under HG conditions, suggesting that visfatin has a potent effect on retinal neovascularization and its mechanism may be associated with the promotion of VEGF and VEGFR‑2 expression under HG conditions.
View Figures
View References

Related Articles

Journal Cover

April-2021
Volume 21 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

  • Journal Metrics
  • Impact Factor: 2.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
International Symposium on Molecular Medicine Conference Banner
The Cancer Story
World Congress on Advances in Oncology and International Symposium on Molecular Medicine Conference Banner
World Academy Of Sciences Banner
Heyday Road Banner

0

Copy and paste a formatted citation
x
Spandidos Publications style
Chen D, Wang Y, Liu M, Cheng J, Liu Z, Song Y and Du J: Visfatin promotes angiogenesis of RF/6A cells through upregulation of VEGF/VEGFR‑2 under high‑glucose conditions. Exp Ther Med 21: 389, 2021
APA
Chen, D., Wang, Y., Liu, M., Cheng, J., Liu, Z., Song, Y., & Du, J. (2021). Visfatin promotes angiogenesis of RF/6A cells through upregulation of VEGF/VEGFR‑2 under high‑glucose conditions. Experimental and Therapeutic Medicine, 21, 389. https://doi.org/10.3892/etm.2021.9820
MLA
Chen, D., Wang, Y., Liu, M., Cheng, J., Liu, Z., Song, Y., Du, J."Visfatin promotes angiogenesis of RF/6A cells through upregulation of VEGF/VEGFR‑2 under high‑glucose conditions". Experimental and Therapeutic Medicine 21.4 (2021): 389.
Chicago
Chen, D., Wang, Y., Liu, M., Cheng, J., Liu, Z., Song, Y., Du, J."Visfatin promotes angiogenesis of RF/6A cells through upregulation of VEGF/VEGFR‑2 under high‑glucose conditions". Experimental and Therapeutic Medicine 21, no. 4 (2021): 389. https://doi.org/10.3892/etm.2021.9820