Open Access

Total astragalosides decrease apoptosis and pyroptosis by inhibiting enterovirus 71 replication in gastric epithelial cells

  • Authors:
    • Xiaoyan Zhang
    • Jinfang Hao
    • Chenxi Sun
    • Jianping Du
    • Qian Han
    • Qingshan Li
  • View Affiliations

  • Published online on: January 24, 2022     https://doi.org/10.3892/etm.2022.11162
  • Article Number: 237
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Enterovirus 71 (EV71) is one of the primary pathogens involved in severe hand, foot and mouth disease in children. EV71 infection causes various types of programmed cell death. However, there are currently no clinically approved specific antiviral drugs for control of EV71 infection. Astragalus membranaceus (AM), a Traditional Chinese medicine, has been used in antiviral therapy in China. The aim of the present study was to determine whether total astragalosides (ASTs), bioactive components of AM, protect against EV. DAPI nuclear staining was used to observe morphological changes of the nucleus and the protective effect of ASTs, which revealed that the nucleus shrank following EV71 infection, while ASTs reversed it. Cell Counting Kit‑8 assay found that human normal gastric epithelial cell (GES‑1 cell) viability decreased following EV71 infection, while lactate dehydrogenase (LDH) assay showed that EV71 infection induced GES‑1 cell damage. Western blotting was used to measure the expression levels of apoptosis and pyroptosis marker protein to determine whether EV71 infection induced apoptosis and pyroptosis in GES‑1 cells. Reverse transcription‑quantitative PCR was used to determine the anti‑EV71 effect of ASTs. The results showed that ASTs protected GES‑1 cells from EV71‑induced cell apoptosis and pyroptosis. Furthermore, the present data demonstrated that the protective effect of ASTs was exerted by suppressing EV71 replication and release. These findings suggested that ASTs may represent a potential antiviral agent for the treatment of EV71 infection.
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March-2022
Volume 23 Issue 3

Print ISSN: 1792-0981
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Spandidos Publications style
Zhang X, Hao J, Sun C, Du J, Han Q and Li Q: Total astragalosides decrease apoptosis and pyroptosis by inhibiting enterovirus 71 replication in gastric epithelial cells. Exp Ther Med 23: 237, 2022.
APA
Zhang, X., Hao, J., Sun, C., Du, J., Han, Q., & Li, Q. (2022). Total astragalosides decrease apoptosis and pyroptosis by inhibiting enterovirus 71 replication in gastric epithelial cells. Experimental and Therapeutic Medicine, 23, 237. https://doi.org/10.3892/etm.2022.11162
MLA
Zhang, X., Hao, J., Sun, C., Du, J., Han, Q., Li, Q."Total astragalosides decrease apoptosis and pyroptosis by inhibiting enterovirus 71 replication in gastric epithelial cells". Experimental and Therapeutic Medicine 23.3 (2022): 237.
Chicago
Zhang, X., Hao, J., Sun, C., Du, J., Han, Q., Li, Q."Total astragalosides decrease apoptosis and pyroptosis by inhibiting enterovirus 71 replication in gastric epithelial cells". Experimental and Therapeutic Medicine 23, no. 3 (2022): 237. https://doi.org/10.3892/etm.2022.11162