Understanding orthopedic infections through a different perspective: Microcalorimetry growth curves

  • Authors:
    • Mihnea Popa
    • Adrian Cursaru
    • Vlad Popa
    • Alexandru Munteanu
    • Bogdan Șerban
    • Bogdan Crețu
    • Sergiu Iordache
    • Catalin Gabriel Smarandache
    • Carmen Orban
    • Cătălin Cîrstoiu
  • View Affiliations

  • Published online on: February 4, 2022     https://doi.org/10.3892/etm.2022.11189
  • Article Number: 263
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Abstract

Infectious disease is a serious healthcare problem in orthopedics, as well as other surgical specialties. Accurate and prompt diagnosis, as well as proper care, is critical, as infection of a surgical wound, particularly in the case of arthroplasties or the use of orthopedic implants, can have a catastrophic effect in most cases, necessitating the removal of foreign material. Lyophilized bacteria samples were obtained from Cantacuzino National Institute of Research and Development for Microbiology and Immunology and investigated microcalorimetrically. Isothermal microcalorimetry measures the temperature generated by the multiplication of microorganisms; using an adapted program, it describes the growth curve in real‑time according to the received electrical signal. The thermograms of Escherichia coli and Klebsiella pneumonie were analyzed, and similarities were observed for both the time required for the bacteria to grow and the heat flow generated by their growth. Bacterial microcalorimetry has a variety of benefits, and should be regarded as a means of rapid and accurate diagnosis. Sensitivity is a valuable attribute for a diagnostic technique; when only a few microorganisms are present, microcalorimetric signs of bacterial multiplication can be observed. Microcalorimetry has potential as a simple diagnostic tool in a variety of infections, but further research is needed to ensure that it is used correctly. A thorough investigation (including kinetic analysis) of a reproducible thermal signal of bacterial growth could lead to the creation of new methods for quickly identifying bacteria.
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April-2022
Volume 23 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Popa M, Cursaru A, Popa V, Munteanu A, Șerban B, Crețu B, Iordache S, Smarandache CG, Orban C, Cîrstoiu C, Cîrstoiu C, et al: Understanding orthopedic infections through a different perspective: Microcalorimetry growth curves. Exp Ther Med 23: 263, 2022.
APA
Popa, M., Cursaru, A., Popa, V., Munteanu, A., Șerban, B., Crețu, B. ... Cîrstoiu, C. (2022). Understanding orthopedic infections through a different perspective: Microcalorimetry growth curves. Experimental and Therapeutic Medicine, 23, 263. https://doi.org/10.3892/etm.2022.11189
MLA
Popa, M., Cursaru, A., Popa, V., Munteanu, A., Șerban, B., Crețu, B., Iordache, S., Smarandache, C. G., Orban, C., Cîrstoiu, C."Understanding orthopedic infections through a different perspective: Microcalorimetry growth curves". Experimental and Therapeutic Medicine 23.4 (2022): 263.
Chicago
Popa, M., Cursaru, A., Popa, V., Munteanu, A., Șerban, B., Crețu, B., Iordache, S., Smarandache, C. G., Orban, C., Cîrstoiu, C."Understanding orthopedic infections through a different perspective: Microcalorimetry growth curves". Experimental and Therapeutic Medicine 23, no. 4 (2022): 263. https://doi.org/10.3892/etm.2022.11189