Open Access

Circular RNA hsa_circ_0011946 promotes the malignant process of salivary adenoid cystic carcinoma by downregulating miR‑1205 expression

  • Authors:
    • Huiping Wei
    • Jun Li
    • Cheng Xie
    • Hao Dong
  • View Affiliations

  • Published online on: February 18, 2022     https://doi.org/10.3892/etm.2022.11224
  • Article Number: 295
  • Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Circular RNA (circRNA/circ) hsa_circ_0011946 has been reported to serve an important role in a number of cancer types; however, to the best of our knowledge, its role in salivary adenoid cystic carcinoma (SACC) has not been reported. In the present study, the primary focus was the effects of hsa_circ_0011946 on the invasion, migration and epithelial‑mesenchymal transformation (EMT) of SACC cells, and the specific mechanisms involved. The expression levels of hsa_circ_0011946 and microRNA (miR)‑1205 in cancer tissues and paracancerous tissues of patients with SACC were analyzed using reverse transcription‑quantitative (RT‑q)PCR. The cell proliferation rate was determined using a Cell Counting Kit‑8 assay. Wound healing assays were performed to analyze the cell migratory ability, while a transwell assay was used to measure the cell invasion ability. Western blotting was used to analyze the expression levels of EMT‑related proteins. Cell transfection was used to knockdown hsa_circ_0011946 and knockdown or overexpress miR‑1205. Subcellular localization assays for hsa_circ_0011946 were performed using RT‑qPCR. A dual‑luciferase reporter gene assay was used to verify the binding between hsa_circ_0011946 and miR‑1205. The results of the present study revealed that the expression levels of hsa_circ_0011946 were significantly upregulated in cancer tissues from patients with SACC. The knockdown of hsa_circ_0011946 expression inhibited the proliferation, invasion and migration of SACC cells, thereby inhibiting the EMT process, which was achieved by downregulating miR‑1205 expression. In conclusion, circRNA hsa_circ_0011946 was discovered to promote the malignant process of SACC by downregulating miR‑1205 expression.
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April-2022
Volume 23 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Wei H, Li J, Xie C and Dong H: Circular RNA hsa_circ_0011946 promotes the malignant process of salivary adenoid cystic carcinoma by downregulating miR‑1205 expression. Exp Ther Med 23: 295, 2022.
APA
Wei, H., Li, J., Xie, C., & Dong, H. (2022). Circular RNA hsa_circ_0011946 promotes the malignant process of salivary adenoid cystic carcinoma by downregulating miR‑1205 expression. Experimental and Therapeutic Medicine, 23, 295. https://doi.org/10.3892/etm.2022.11224
MLA
Wei, H., Li, J., Xie, C., Dong, H."Circular RNA hsa_circ_0011946 promotes the malignant process of salivary adenoid cystic carcinoma by downregulating miR‑1205 expression". Experimental and Therapeutic Medicine 23.4 (2022): 295.
Chicago
Wei, H., Li, J., Xie, C., Dong, H."Circular RNA hsa_circ_0011946 promotes the malignant process of salivary adenoid cystic carcinoma by downregulating miR‑1205 expression". Experimental and Therapeutic Medicine 23, no. 4 (2022): 295. https://doi.org/10.3892/etm.2022.11224