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Osteoprotegerin/receptor activator of nuclear factor‑κB ligand are involved in periodontitis‑promoted vascular calcification

  • Authors:
    • Mengyu Jiao
    • Pengmei Zhang
    • Xinbo Yu
    • Pei Sun
    • Meiwei Liu
    • Yanya Qiao
    • Keqing Pan
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China
    Copyright: © Jiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 512
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    Published online on: June 14, 2022
       https://doi.org/10.3892/etm.2022.11439
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Abstract

The present study explored the potential role of osteoprotegerin (OPG)/receptor activator of nuclear factor‑κB (RANK)/receptor activator of nuclear factor‑κB ligand (RANKL) in promoting vascular calcification by periodontitis. Thirty‑six male Wistar rats were randomly assigned to four groups to establish animal models as follows: the sham group (group C), vascular calcification group (group VDN), periodontitis group (group CP), and test group (group CP+VDN). After eight weeks, all the rats were sacrificed. The periodontal and vascular calcification indices were detected. Quantitative polymerase chain reaction (qPCR), immunohistochemistry, western blot analysis, and enzyme‑linked immunosorbent assay (ELISA) were used to quantify OPG/RANK/RANKL expression in vascular tissue and serum. Protein expression analyses revealed the expression of OPG and RANKL in the vascular tissues of the four groups. The expression of OPG in group C was the highest, which was similar to group CP+VDN, and the expression of OPG in groups CP and VDN were lower. However, the expression of RANKL was inversely correlated with OPG, and the ratio of RANKL/OPG was also higher in groups CP and VDN than that in groups C and CP+VDN. In conclusion, OPG/RANK/RANKL may play an essential role in the promotion of vascular calcification by periodontitis. However, the expression levels of OPG and RANKL were not simply superimposed when periodontitis and vascular calcification co‑existed.
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Copy and paste a formatted citation
Spandidos Publications style
Jiao M, Zhang P, Yu X, Sun P, Liu M, Qiao Y and Pan K: Osteoprotegerin/receptor activator of nuclear factor‑κB ligand are involved in periodontitis‑promoted vascular calcification. Exp Ther Med 24: 512, 2022.
APA
Jiao, M., Zhang, P., Yu, X., Sun, P., Liu, M., Qiao, Y., & Pan, K. (2022). Osteoprotegerin/receptor activator of nuclear factor‑κB ligand are involved in periodontitis‑promoted vascular calcification. Experimental and Therapeutic Medicine, 24, 512. https://doi.org/10.3892/etm.2022.11439
MLA
Jiao, M., Zhang, P., Yu, X., Sun, P., Liu, M., Qiao, Y., Pan, K."Osteoprotegerin/receptor activator of nuclear factor‑κB ligand are involved in periodontitis‑promoted vascular calcification". Experimental and Therapeutic Medicine 24.2 (2022): 512.
Chicago
Jiao, M., Zhang, P., Yu, X., Sun, P., Liu, M., Qiao, Y., Pan, K."Osteoprotegerin/receptor activator of nuclear factor‑κB ligand are involved in periodontitis‑promoted vascular calcification". Experimental and Therapeutic Medicine 24, no. 2 (2022): 512. https://doi.org/10.3892/etm.2022.11439
Copy and paste a formatted citation
x
Spandidos Publications style
Jiao M, Zhang P, Yu X, Sun P, Liu M, Qiao Y and Pan K: Osteoprotegerin/receptor activator of nuclear factor‑κB ligand are involved in periodontitis‑promoted vascular calcification. Exp Ther Med 24: 512, 2022.
APA
Jiao, M., Zhang, P., Yu, X., Sun, P., Liu, M., Qiao, Y., & Pan, K. (2022). Osteoprotegerin/receptor activator of nuclear factor‑κB ligand are involved in periodontitis‑promoted vascular calcification. Experimental and Therapeutic Medicine, 24, 512. https://doi.org/10.3892/etm.2022.11439
MLA
Jiao, M., Zhang, P., Yu, X., Sun, P., Liu, M., Qiao, Y., Pan, K."Osteoprotegerin/receptor activator of nuclear factor‑κB ligand are involved in periodontitis‑promoted vascular calcification". Experimental and Therapeutic Medicine 24.2 (2022): 512.
Chicago
Jiao, M., Zhang, P., Yu, X., Sun, P., Liu, M., Qiao, Y., Pan, K."Osteoprotegerin/receptor activator of nuclear factor‑κB ligand are involved in periodontitis‑promoted vascular calcification". Experimental and Therapeutic Medicine 24, no. 2 (2022): 512. https://doi.org/10.3892/etm.2022.11439
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