Open Access

Protective effect of hydroxysafflor yellow A on renal ischemia‑­reperfusion injury by targeting the Akt‑Nrf2 axis in mice

  • Authors:
    • Yueming Wang
    • Kaiyue Han
    • Zile Li
    • Xiaoxuan Tang
    • Chen Wang
    • Yaxuan Zhao
    • Hengchao Zhang
    • Ziran Geng
    • Jie Kong
    • Xiying Luan
    • Yanlian Xiong
  • View Affiliations

  • Published online on: November 3, 2022     https://doi.org/10.3892/etm.2022.11677
  • Article Number: 741
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ischemic/reperfusion (I/R) injury is the primary cause of acute kidney injury (AKI). Hydroxysafflor yellow A (HSYA), a natural compound isolated from Carthamus tinctorius L., has been found to possess anti‑inflammatory and antioxidant properties. However, the protective effects and potential mechanism of HSYA on I/R‑induced AKI remains unclear. In the present study, the in vitro hypoxia/reoxygenation (H/R) and in vivo renal I/R models were employed to investigate the renal protective effects and molecular mechanisms of HSYA on I/R‑induced AKI. The present results indicated that HSYA pretreatment significantly ameliorated renal damage and dysfunction in the I/R injury mice via enhancing the antioxidant capacity and suppressing the oxidative stress injury, inflammatory response, and apoptosis. Mechanistic studies showed that HSYA could upregulate Akt/GSK‑3β/Fyn‑Nrf2 axis‑mediated antioxidant gene expression both in vitro and in vivo. Moreover, HSYA‑mediated improvement in antioxidant, anti‑inflammatory, and anti‑apoptotic effects in H/R‑treated HK‑2 cells was abrogated by Akt inhibitor LY294002 supplementation. In summary, the present results demonstrated that HSYA attenuated kidney oxidative stress, inflammation response, and apoptosis induced by I/R, at least in part, via activating the Akt/GSK‑3β/Fyn‑Nrf2 axis pathway. These findings provided evidence that HSYA may be applied as a potential therapeutic agent in the treatment of I/R induced AKI.
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December-2022
Volume 24 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang Y, Han K, Li Z, Tang X, Wang C, Zhao Y, Zhang H, Geng Z, Kong J, Luan X, Luan X, et al: Protective effect of hydroxysafflor yellow A on renal ischemia‑­reperfusion injury by targeting the Akt‑Nrf2 axis in mice. Exp Ther Med 24: 741, 2022.
APA
Wang, Y., Han, K., Li, Z., Tang, X., Wang, C., Zhao, Y. ... Xiong, Y. (2022). Protective effect of hydroxysafflor yellow A on renal ischemia‑­reperfusion injury by targeting the Akt‑Nrf2 axis in mice. Experimental and Therapeutic Medicine, 24, 741. https://doi.org/10.3892/etm.2022.11677
MLA
Wang, Y., Han, K., Li, Z., Tang, X., Wang, C., Zhao, Y., Zhang, H., Geng, Z., Kong, J., Luan, X., Xiong, Y."Protective effect of hydroxysafflor yellow A on renal ischemia‑­reperfusion injury by targeting the Akt‑Nrf2 axis in mice". Experimental and Therapeutic Medicine 24.6 (2022): 741.
Chicago
Wang, Y., Han, K., Li, Z., Tang, X., Wang, C., Zhao, Y., Zhang, H., Geng, Z., Kong, J., Luan, X., Xiong, Y."Protective effect of hydroxysafflor yellow A on renal ischemia‑­reperfusion injury by targeting the Akt‑Nrf2 axis in mice". Experimental and Therapeutic Medicine 24, no. 6 (2022): 741. https://doi.org/10.3892/etm.2022.11677