Open Access

Complement factor H inhibits endothelial cell migration through suppression of STAT3 signaling

  • Authors:
    • Jiang Li
    • Hong Huang
    • Shanhu Xu
    • Mengge Fan
    • Kaili Wang
    • Xia Wang
    • Jiao Zhang
    • Shengshi Huang
    • Alex Gatt
    • Ju Liu
  • View Affiliations

  • Published online on: July 10, 2023     https://doi.org/10.3892/etm.2023.12107
  • Article Number: 408
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Complement factor H (CFH), a major soluble inhibitor of complement, is a plasma protein that directly interacts with the endothelium of blood vessels. Mutations in the CFH gene lead to diseases associated with excessive angiogenesis; however, the underlying mechanisms are unknown. The present study aimed to determine the effects of CFH on endothelial cells and to explore the underlying mechanisms. The adenoviral plasmid expressing CFH was transduced into HepG2 cells, and the culture medium supernatant was collected and co‑cultured with human umbilical vein endothelial cells (HUVECs). Cell proliferation was measured by CCK8 and MTT assays, and cell migration was measured by wound healing and Transwell assays. Reverse transcription‑quantitative PCR was performed to detect gene transcription. Western blotting was used to determine protein levels. The results revealed that CFH can inhibit migration, but not viability, of HUVECs. In addition, CFH did not significantly alter MAPK or TGF‑β signaling, whereas it decreased STAT3 phosphorylation in HUVECs. Furthermore, CFH failed to reduce migration of HUVECs, with inhibition of STAT3 signaling by STATTIC or activation of STAT3 signaling by overexpression of STAT3 (Y705D) compromising CFH‑inhibited HUVEC migration. CFH also decreased the expression levels of vascular endothelial growth factor receptor 2, a downstream effector of STAT3 mediating endothelial cell migration. In conclusion, the present study suggested that CFH may be a potential therapeutic target for angiogenesis‑related diseases.
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August-2023
Volume 26 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Li J, Huang H, Xu S, Fan M, Wang K, Wang X, Zhang J, Huang S, Gatt A, Liu J, Liu J, et al: Complement factor H inhibits endothelial cell migration through suppression of STAT3 signaling. Exp Ther Med 26: 408, 2023
APA
Li, J., Huang, H., Xu, S., Fan, M., Wang, K., Wang, X. ... Liu, J. (2023). Complement factor H inhibits endothelial cell migration through suppression of STAT3 signaling. Experimental and Therapeutic Medicine, 26, 408. https://doi.org/10.3892/etm.2023.12107
MLA
Li, J., Huang, H., Xu, S., Fan, M., Wang, K., Wang, X., Zhang, J., Huang, S., Gatt, A., Liu, J."Complement factor H inhibits endothelial cell migration through suppression of STAT3 signaling". Experimental and Therapeutic Medicine 26.2 (2023): 408.
Chicago
Li, J., Huang, H., Xu, S., Fan, M., Wang, K., Wang, X., Zhang, J., Huang, S., Gatt, A., Liu, J."Complement factor H inhibits endothelial cell migration through suppression of STAT3 signaling". Experimental and Therapeutic Medicine 26, no. 2 (2023): 408. https://doi.org/10.3892/etm.2023.12107