Transcriptome analysis of hydrogen inhibits osteoclastogenesis of mouse bone marrow mononuclear cells

Liu,Yong; Wang,Wei; Zeng,Yong; Zeng,Hui

doi:10.3892/etm.2023.12135

Transcriptome analysis of hydrogen inhibits osteoclastogenesis of mouse bone marrow mononuclear cells

Authors:
- Yong Liu
- Wei Wang
- Yong Zeng
- Hui Zeng
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Affiliations: Department of Orthopedics, Chengdu Second People's Hospital, Chengdu, Sichuan 610017, P.R. China, Department of Human Anatomy and Histoembryology, Zhuhai Campus of Zunyi Medical University, Zhuhai, Guangdong 519041, P.R. China, Department of Bone and Joint Surgery, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China
Published online on: July 31, 2023 https://doi.org/10.3892/etm.2023.12135
Article Number: 436
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hydrogen (H₂) is a major biodegradation product of implanted magnesium (Mg) alloys that are commonly used in the healing of bone fractures. Our earlier study showed that H₂ can inhibit mouse bone marrow mononuclear cell (BMMC) osteoclastogenesis during the differentiation of these cells into osteoclasts, thereby facilitating fracture healing. However, the way by which H2 inhibits osteoclastogenesis remains to be elucidated. The present study used RNA‑sequencing to study the transcriptome of H₂‑exposed BMMCs in an osteoclast‑induced environment and identified the target genes and signaling pathways through which H2 exerts its biological effects. Several upregulated genes were identified: Fos, Dusp1, Cxcl1, Reln, Itga2b, Plin2, Lif, Thbs1, Vegfa and Gadd45a. Several downregulated genes were also revealed: Hspa1b, Gm4951, F830016B08Rik, Fads2, Hspa1a, Slc27a6, Cacna1b, Scd2, Lama3 and Col4a5. These differentially expressed genes were mainly involved in osteoclast differentiation cascades, as well as PI3K‑AKT, Forkhead box O (FoxO), MAPK, peroxisome proliferator‑activated receptor (PPAR), TNF, TGF‑β, JAK‑STAT, RAS, VEGF, hypoxia‑inducible factor (HIF‑1) and AMPK signaling pathways. In summary, the present study revealed the key genes and signaling pathways involved in the H2‑mediated inhibition of osteoclastogenesis, thereby providing a theoretical basis for the significance of H₂ and an experimental basis for the application of Mg alloys in the treatment of osteoporosis.

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