<em>Humulus japonicus</em> ameliorates irritant contact dermatitis by suppressing NF‑κB p65‑dependent inflammatory responses in mice

Kim,Yu-Bin; Kang,Eun-Jung; Noh,Jung-Ran; An,Jin-Pyo; Park,Jong-Tae; Oh,Won Keun; Kim,Yong-Hoon; Lee,Chul-Ho

doi:10.3892/etm.2023.12145

Humulus japonicus ameliorates irritant contact dermatitis by suppressing NF‑κB p65‑dependent inflammatory responses in mice

This article is part of the special Issue: Immuno-Dermatology
Authors:
- Yu-Bin Kim
- Eun-Jung Kang
- Jung-Ran Noh
- Jin-Pyo An
- Jong-Tae Park
- Won Keun Oh
- Yong-Hoon Kim
- Chul-Ho Lee
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Affiliations: Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea, Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea, Department of Food Science and Technology, Chungnam National University, Daejeon 34134, Republic of Korea
Published online on: August 2, 2023 https://doi.org/10.3892/etm.2023.12145
Article Number: 446
Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

As a type of contact dermatitis (CD), irritant CD (ICD) is an acute skin inflammation caused by external irritants, such as soap, water and chemicals. Humulus japonicus (HJ) is a herbal medicine widely distributed in Asian countries and has anti‑inflammatory, antimicrobial and antioxidant effects. The current study aimed to investigate the anti‑dermatitis effect of HJ on ICD and determine the molecular basis of this effect using 12‑O‑tetradecanoylphorbol‑13‑acetate (TPA)‑induced dermatitis mice models and lipopolysaccharide (LPS)‑stimulated RAW264.7 cells. Mice were orally administered HJ and luteolin, the major compound in HJ, and topically administered TPA on the right ear to induce dermatitis. Topical application of TPA induced ear redness, oedema and increased infiltration of neutrophils and macrophages, which ameliorated following HJ and luteolin administration. The gene expression levels of inflammatory cell migrating chemokines, chemokine ligand 3 (CCL3) and chemokine (C‑X‑C motif) ligand 2 (CXCL2), and pro‑inflammatory cytokine, IL‑1β, were reduced in the ears of HJ‑ and luteolin‑treated mice. HJ and luteolin also inhibited the gene expression of chemokines, CCL3 and CXCL2, and pro‑inflammatory cytokines, IL‑1β, IL‑6 and TNF‑α, in LPS‑stimulated RAW264.7 cells. Moreover, HJ and luteolin decreased the expression levels of two key inflammatory enzymes, cyclooxygenase‑2 (COX2) and inducible nitric oxide synthase (iNOS), and total and active phosphorylation of NF‑κB p65. These results suggest that HJ could have a protective effect against ICD by suppressing inflammatory responses; therefore, HJ is a promising therapeutic strategy for ICD treatment.

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