Open Access

Effects of DJ‑1 on apoptosis and mitophagy of glomerular podocytes

  • Authors:
    • Jing Xiao
    • Junjie Tan
    • Li Yu
    • Guosheng Liu
    • Shengyou Yu
  • View Affiliations

  • Published online on: August 11, 2023     https://doi.org/10.3892/etm.2023.12162
  • Article Number: 463
  • Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

By studying the effects of DJ‑1 overexpression and silencing on the morphological structure and mitophagy of glomerular podocytes, the present study aimed to identify the effects of DJ‑1 on glomerular podocyte apoptosis and mitophagy. MPC5 mouse glomerular podocytes were cultured in vitro and divided into four groups: Control, DJ‑1 overexpression, empty vector and DJ‑1 silencing. DJ‑1 gene overexpression and silencing models were prepared, the morphological structures of podocytes and mitochondria in each group were observed, and podocyte apoptosis and DJ‑1/PTEN expression were subsequently detected in each group. The experimental results showed reduced volume, retracted foot processes, loosened intercellular connections, presence of dead cells, increased apoptotic rate, increased expression of PTEN, and swollen mitochondria due to the number of vacuoles and autophagosomes in podocytes in the DJ‑1 silencing group. The surface areas of podocytes in the DJ‑1 overexpression group were greater than those in the control group. Moreover, the structure of the foot processes was more obvious, the number of cells was greater, the intercellular connections were closer, the apoptotic rate was reduced, the expression of PTEN was decreased, the mitochondrial structure was more obvious and the mitochondrial cristae were more whole. Notably, there were no differences between the empty vector and control groups. In conclusion, these results indicated that DJ‑1 may regulate podocyte apoptosis and mitophagy through the DJ‑1/PTEN pathway, and could maintain the stability of the normal morphology, structure and function of glomerular podocytes.
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October-2023
Volume 26 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Xiao J, Tan J, Yu L, Liu G and Yu S: Effects of DJ‑1 on apoptosis and mitophagy of glomerular podocytes. Exp Ther Med 26: 463, 2023
APA
Xiao, J., Tan, J., Yu, L., Liu, G., & Yu, S. (2023). Effects of DJ‑1 on apoptosis and mitophagy of glomerular podocytes. Experimental and Therapeutic Medicine, 26, 463. https://doi.org/10.3892/etm.2023.12162
MLA
Xiao, J., Tan, J., Yu, L., Liu, G., Yu, S."Effects of DJ‑1 on apoptosis and mitophagy of glomerular podocytes". Experimental and Therapeutic Medicine 26.4 (2023): 463.
Chicago
Xiao, J., Tan, J., Yu, L., Liu, G., Yu, S."Effects of DJ‑1 on apoptosis and mitophagy of glomerular podocytes". Experimental and Therapeutic Medicine 26, no. 4 (2023): 463. https://doi.org/10.3892/etm.2023.12162