Taurine accelerates the synthesis of ceramides and hyaluronic acid in cultured epidermis and dermal fibroblasts

Yoshimura,Tomohisa; Manabe,Chika; Nagumo,Jun-Ichiro; Nagahama,Tohru; Sato,Takashi; Murakami,Shigeru

doi:10.3892/etm.2023.12211

Taurine accelerates the synthesis of ceramides and hyaluronic acid in cultured epidermis and dermal fibroblasts

Authors:
- Tomohisa Yoshimura
- Chika Manabe
- Jun-Ichiro Nagumo
- Tohru Nagahama
- Takashi Sato
- Shigeru Murakami
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Affiliations: R&D Laboratories, Department of Self‑Medication, Taisho Pharmaceutical Co., Ltd., Saitama, Saitama 331‑9530, Japan, Department of Biochemistry, Tokyo University of Pharmacy and Life Sciences, Tokyo 192‑0392, Japan, Department of Bioscience and Biotechnology, Fukui Prefectural University, Eiheiji, Fukui 910‑1195, Japan
Published online on: September 20, 2023 https://doi.org/10.3892/etm.2023.12211
Article Number: 512
Copyright: © Yoshimura et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Taurine is a sulfur‑containing amino acid derivative that can be found in the majority of mammalian tissues. Taurine is also present in the skin and is involved in maintaining skin homeostasis by exerting osmoregulatory and antioxidant effects. Previous studies have indicated that taurine treatment is effective against age‑, ultraviolet‑ or detergent‑induced skin dysfunction. To determine the mechanism responsible for the beneficial actions of taurine in the skin, the present study aimed to evaluate the effects of taurine on epidermal components (ceramides and filaggrin) and on the dermal extracellular matrix, in three‑dimensionally (3D) cultured epidermis and dermal fibroblasts, respectively. These cells were cultured in the presence of 3‑50 mM taurine, and cells or culture medium were collected for analysis. The effects of taurine on transepidermal water loss (TEWL) in the skin and the expression of inflammatory cytokines, including IL‑1α, IL‑1β and IL‑1 receptor antagonist, were investigated in acetone‑treated 3D‑cultured epidermis using a Tewameter and reverse transcription‑quantitative PCR (RT‑qPCR), respectively. The mRNA expression levels of MMP‑1 and hyaluronic acid (HA) production were measured in skin dermal fibroblasts using RT‑qPCR and ELISA, respectively. Taurine was found to suppress acetone‑induced elevation in TEWL in 3D‑cultured epidermis. Taurine also stimulated the mRNA expression of ceramide synthase 4 and filaggrin, a major structural protein in the stratum corneum, in 3D‑cultured epidermis. In skin dermal fibroblasts, taurine inhibited the IL‑1α‑stimulated mRNA and protein expression of MMP‑1. In addition, taurine treatment increased HA synthase‑2 mRNA expression and in turn HA production. Results from the present study suggest that the protective effect of taurine on the skin is associated with the enhancement of epidermal barrier component expression and modulation of dermal extracellular matrix metabolism.

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