IGF2BP2‑dependent STIM1 inhibition protects against LPS‑induced pneumonia <em>in vitro</em> by alleviating endoplasmic reticulum stress and the inflammatory response

Zhou,Wei; Dai,Qigang; Su,Ning; Liu,Zhihui; Hu,Jinxing

doi:10.3892/etm.2023.12273

IGF2BP2‑dependent STIM1 inhibition protects against LPS‑induced pneumonia in vitro by alleviating endoplasmic reticulum stress and the inflammatory response

Authors:
- Wei Zhou
- Qigang Dai
- Ning Su
- Zhihui Liu
- Jinxing Hu
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Affiliations: Department of Pathology, Guangzhou Chest Hospital, Guangzhou, Guangdong 510095, P.R. China, Department of Oncology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510699, P.R. China, Department of Oncology, Guangzhou Chest Hospital, Guangzhou, Guangdong 510095, P.R. China, Department of Clinical Laboratory, Guangzhou Chest Hospital, Guangzhou, Guangdong 510095, P.R. China, Department of Tuberculosis, Guangzhou Chest Hospital, Guangzhou, Guangdong 510095, P.R. China
Published online on: October 26, 2023 https://doi.org/10.3892/etm.2023.12273
Article Number: 575
Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Pneumonia is a disease caused by inflammation and has high morbidity and mortality rates. Stromal interaction molecule 1 (STIM1) is involved in the regulation of inflammatory processes. However, to the best of the authors' knowledge, the role of STIM1 in pneumonia has not yet been reported. In the present study, lipopolysaccharide (LPS) was administered to A549 cells to construct a cell damage model. The expression of STIM1 in the model cells was detected by western blotting and reverse transcription‑quantitative PCR. Then, STIM1 expression was inhibited and cell survival was detected by Cell Counting Kit‑8 and flow cytometry. The expression of inflammatory factors was detected by enzyme‑linked immunosorbent assay and endoplasmic reticulum stress (ERS)‑related proteins were detected by immunofluorescence and western blotting. Subsequently, the relationship between insulin‑like growth factor 2 mRNA binding protein 2 (IGF2BP2) and STIM1 was verified by RNA‑binding protein immunoprecipitation assay and actinomycin D treatment. Finally, the regulatory mechanism of IGF2BP2 and STIM1 in LPS‑induced A549 cells was further investigated. The results of the present study demonstrated that STIM1 expression was increased in LPS‑induced A549 cells and that STIM1 knockdown inhibited LPS‑induced A549 cell apoptosis and alleviated LPS‑induced A549 cell inflammation and ERS. In addition, IGF2BP2 enhanced the stability of STIM1 mRNA and knockdown of IGF2BP2‑regulated STIM1 expression alleviated LPS‑induced ERS and inflammatory responses in A549 cells. In conclusion, knockdown of IGF2BP2‑regulated STIM1 improved cell damage in the LPS‑induced pneumonia cell model by alleviating ERS and the inflammatory response.

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