Anti‑inflammatory effects of methanol extract from <em>Peperomia dindygulensis</em> Miq. mediated by HO‑1 in LPS‑induced RAW 264.7 cells

Min,Won-Hong; Ko,Chae-Yeon; Kim,Hyemin; Kwon,Hyuk-Kwon; Jang,Hyun-Jae; Bach,Tran The; Han,Le Ngoc; Lee,Jeong-Hyung; Kim,Hyo-Jin; Hwangbo,Cheol

doi:10.3892/etm.2024.12606

Anti‑inflammatory effects of methanol extract from Peperomia dindygulensis Miq. mediated by HO‑1 in LPS‑induced RAW 264.7 cells

Authors:
- Won-Hong Min
- Chae-Yeon Ko
- Hyemin Kim
- Hyuk-Kwon Kwon
- Hyun-Jae Jang
- Tran The Bach
- Le Ngoc Han
- Jeong-Hyung Lee
- Hyo-Jin Kim
- Cheol Hwangbo
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Affiliations: Division of Life Science, College of Natural Sciences, Gyeongsang National University, Jinju‑si, Gyeongsang 52828, Republic of Korea, Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheonju‑si, Chungcheongbuk‑do 28116, Republic of Korea, Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Cau Giay, Hanoi 01211, Vietnam, Department of Biochemistry (BK21 Four), College of Natural Sciences, Kangwon National University, Chuncheon, Gangwon 24414, Republic of Korea
Published online on: June 14, 2024 https://doi.org/10.3892/etm.2024.12606
Article Number: 317
Copyright: © Min et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Inflammation serves as a multifaceted defense mechanism activated by pathogens, cellular damage and irritants, aiming to eliminate primary causes of injury and promote tissue repair. Peperomia dindygulensis Miq. (P. dindygulensis), prevalent in Vietnam and southern China, has a history of traditional use for treating cough, fever and asthma. Previous studies on its phytochemicals have shown their potential as anti‑inflammatory agents, yet underlying mechanisms remain to be elucidated. The present study investigated the regulatory effects of P. dindygulensis on the anti‑inflammatory pathways. The methanol extracts of P. dindygulensis (PDME) were found to inhibit nitric oxide (NO) production and induce heme oxygenase‑1 (HO‑1) expression in murine macrophages. While MAPKs inhibitors, such as SP600125, SB203580 and U0126 did not regulate HO‑1 expression, the treatment of cycloheximide, a translation inhibitor, reduced HO‑1. Furthermore, PDME inhibited lipopolysaccharide (LPS)‑induced inducible nitric oxide synthase (iNOS), cyclooxygenase‑2 (COX‑2) and TNF‑α expression at both the mRNA and protein levels. The activity of NOS and the expression of TNF‑α, iNOS and COX‑2 decreased in LPS‑stimulated Raw 264.7 cells treated with PDME and this effect was regulated by inhibition of HO‑1 activity. These findings suggested that PDME functions as an HO‑1 inducer and serves as an effective natural anti‑inflammatory agent in LPS‑induced inflammation.

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