Evaluation of the microvascular density in astrocytomas in adults correlated using SPECT-MIBI

Pantoja Cavalcante,Sandro; De Almeida,José R.W.; Clara,Carlos A.; Scapulatempo Neto,Cristovam; Verzinhase Peres,Stela; Moriguchi,Sônia M.; Dos Santos,Marcelo J.; Da Rocha,Euclides T.

doi:10.3892/etm_00000045

Evaluation of the microvascular density in astrocytomas in adults correlated using SPECT-MIBI

Authors:
- Sandro Pantoja Cavalcante
- José R.W. De Almeida
- Carlos A. Clara
- Cristovam Scapulatempo Neto
- Stela Verzinhase Peres
- Sônia M. Moriguchi
- Marcelo J. Dos Santos
- Euclides T. Da Rocha
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Affiliations: Hospital de Câncer - Fundação Pio XII, 14784-400 Barretos, São Paulo, Brazil
Published online on: March 1, 2010 https://doi.org/10.3892/etm_00000045
Pages: 293-299

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Abstract

Microvascular density (MVD) may be an additional prognostic marker for astrocytomas, but the heterogeneity of these tumors limits its use. Thus, imaging examinations such as SPECT-MIBI (2-methoxyisobutyl isonitrile) may take on an indirect role in astrocytoma evaluation. The aim of this study was to evaluate MVD in astrocytomas using immunohistochemistry with anti-CD34 monoclonal antibodies. The relationship between the immunohistochemical data and the parameters obtained from SPECT-MIBI was evaluated. This cross-sectional study evaluated 48 patients with brain tumors including low-grade astrocytomas (LGAs), anaplastic astrocytomas (AAs) and glioblastoma multiformes (GBMs). Patients had been admitted to the Hospital de Câncer de Barretos - Fundação Pio XII, and underwent brain SPECT-MIBI prior to any treatment. MVD was determined under an optical microscope by counting microvessels on slides from each case. SPECT-MIBI images were analyzed visually and semiquantitatively. GBMs, AAs and LGAs represented 50, 16.7 and 33.3% of the total sample, respectively. There were 13 normal and 35 abnormal SPECT-MIBI images. Significant differences in MVD were found between AA and LGA cases (p=0.040), but not between normal and abnormal SPECT-MIBI. The mean counts from SPECT-MIBI were not correlated with MVD. Among the GBM cases, there were no significant findings, except for an increased likelihood of abnormal histological test results. MVD was related to histological grade (in AA and LGA cases) but was not correlated with SPECT-MIBI.

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1.	Kleihues P, Louis DN, Scheithauer BW, Rorke LB, Reifenberger G, Burger PC and Cavanee WK: The WHO classification of tumors of the nervous system. J Neuropathol Exp Neurol. 6:215–225. 2002.
2.	Lois DN, Ohgaki H, Wiestler OD and Cavanee WK: WHO Classification of Tumors of the Central Nervous System. IARC; Lyon: 2007
3.	Birlik B, Canda S and Ozer E: Tumour vascularity is of prognostic significance in adult, but not paediatric astrocytomas. Neuropathol Appl Neurobiol. 32:532–538. 2006. View Article : Google Scholar : PubMed/NCBI
4.	Weidner N, Semple JP, Welch WR and Folkman J: Tumor angiogenesis and metastasis – correlation in invasive breast carcinoma. N Eng J Med. 324:1–8. 1991.
5.	Tanaka F, Otake Y, Yanagihara K, et al: Evaluation of angiogenesis in non-small cell lung cancer: Comparison between anti-CD34 antibody and anti-CD105 antibody. Clin Cancer Res. 7:3410–3415. 2001.PubMed/NCBI
6.	Offersen B, Pfeiffer P, Hamilton-Dutoit S and Overgaad J: Patterns of angiogenesis in non-small cell lung carcinoma. Cancer. 91:1500–1509. 2001. View Article : Google Scholar
7.	Ding S, Li C, Lin S, et al: Comparative evaluation of microvessel density determined by CD34 or CD105 in benign and malignant gastric lesions. Hum Pathol. 37:861–866. 2006. View Article : Google Scholar : PubMed/NCBI
8.	Amar A, Giovanini AF, Rosa MP, Yamassaki HO, De Carvalho MB and Rapoport A: Densidade microvascular no carcinoma de língua. Rev Assoc Med Bras. 48:204–208. 2002.
9.	Leon SP, Folkerth RD and Black PM: Microvessel density is a prognostic indicator for patients with astroglial brain tumors. Cancer. 77:362–372. 1996. View Article : Google Scholar : PubMed/NCBI
10.	Yao Y, Kubota T, Takeuchi H and Sato K: Prognostic significance of microvessel density determined by an anti-CD105/endoglin monoclonal antibody in astrocytic tumors: comparison with an anti-CD31 monoclonal antibody. Neuropathology. 25:201–206. 2005. View Article : Google Scholar : PubMed/NCBI
11.	Baldari S, Pecorella R, Cosentino S and Minutoli F: Investigation of brain tumours with 99mTc-MIBI SPECT. Q J Nucl Med. 46:336–345. 2002.PubMed/NCBI
12.	Goethals I, De Winter O, Dierckk R, Annovazzi A, Signore A and van de Wiele C: False-negative Tc-99m scintigraphy in histopathologically proved recurrent high-grade oligodendroglioma. Clin Nucl Med. 28:299–301. 2003. View Article : Google Scholar : PubMed/NCBI
13.	Minutoli F, Angileri FF, Conti A, et al: Timing of examination affects reliability of 99mTc-methoxyisobutylisonitrile SPECT in distinguishing neoplastic from nonneoplastic brain hematomas. J Nucl Med. 46:574–579. 2005.
14.	Andrews DW, Das R, Kim S, Zhang J and Curtis M: Technetium-MIBI as a glioma imaging agent for the assessment of multidrug resistance. Neurosurgery. 40:1323–1332. 1997. View Article : Google Scholar : PubMed/NCBI
15.	Sasajima T, Shimada N, Naitoh Y, Takahashi M, Hu Y, Satohh T and Mizoi K: 99mTc-MIBI imaging for prediction of therapeutic effects of second-generation MDR1 inhibitors in malignant brain tumors. Int J Cancer. 121:2637–2645. 2007. View Article : Google Scholar : PubMed/NCBI
16.	Staudenherz A, Fazeny B, Marosi C, et al: Does 99mTc-sestamibi in high-grade malignant brain tumors reflect blood-brain barrier damage only? Neuroimage. 12:109–111. 2000. View Article : Google Scholar : PubMed/NCBI
17.	Staudenherz A, Wolfsberg S, Killer M, et al: Microvessel density is not crucial for scintigraphic visualization of brain tumors using 99mTc-MIBI. Microvasc Res. 67:218–222. 2004. View Article : Google Scholar : PubMed/NCBI
18.	Nagamachi S, Jinnouchi S, Nabeshima K, et al: The correlation between 99mTc-MIBI uptake and MIB-1 as a nuclear proliferation marker in glioma – a comparative study with 201TL. Neuroradiology. 43:1023–1030. 2001.PubMed/NCBI
19.	Ak I, Gulbas Z, Altinel F and Vardareli E: TC-99m MIBI uptake and its relation to the proliferative potential of brain tumors. Clin Nucl Med. 28:29–33. 2003. View Article : Google Scholar : PubMed/NCBI
20.	Carvalho PA, Chiu ML, Kronauge JF, Kawamura M, Jones AG, Holman BL and Piwnica-Worms D: Subcellular distribution and analysis of technetium-99-MIBI in isolated perfused rat hearts. J Nucl Med. 33:1516–1522. 1992.PubMed/NCBI
21.	Piwinca-Worms D, Kronauge JF and Chiu ML: Enhancement by tetraphenylborate of technetium-99m-MIBI uptake kinetics and accumulation in cultured chick myocardial cells. J Nucl Med. 32:1992–1999. 1991.PubMed/NCBI
22.	Offersen B, Sorensen F, Yilmaz M, Knoop A and Overgaard J: Chalkley estimates of angiogenesis in early breast cancer. Acta Oncol. 41:695–703. 2002. View Article : Google Scholar : PubMed/NCBI
23.	Mineo TC, Ambrogi V, Baldi A, Rabitti C, Bollero P, Vicenzi B and Tonini G: Prognostic impact of VEGF, CD31, CD34 and CD105 expression and tumor vessel invasion after radical surgery for IB-IIA non-small cell lung cancer. J Clin Pathol. 57:591–597. 2004. View Article : Google Scholar : PubMed/NCBI
24.	Beauchesne P and Soler C: Correlation of 99m Tc-MIBI brain spect (Functional index ratios) and survival after treatment failure in malignant glioma patients. Anticancer Res. 22:3081–3085. 2002.PubMed/NCBI
25.	Soler C, Beauchesne P, Maatougui K, et al: Technetium-99msestamibi brain single-photon emission tomography for detection of recurrent gliomas after radiation therapy. Eur J Nucl Med. 25:1649–1657. 1998. View Article : Google Scholar : PubMed/NCBI
26.	San Pedro EC, Yilmaz M, Liu HG, Rosenfeld SS and Mountz JM: A new semiquantitative method for comparing brain tumor uptake of Tc-99m sestamibi and TL-201. Clin Nucl Med. 24:868–873. 1999.PubMed/NCBI
27.	Field A: Discovering Statistic Using SPSS. 2nd edition. SAGE; California: 2005
28.	Glantz MJ, Burger PC, Herdon JE II, Friedman AH, Caircross JG, Vick NA and Shold SC Jr: Influence of the type of surgery on the histologic diagnosis in patients with anaplastic gliomas. Neurology. 41:1741–1744. 1991. View Article : Google Scholar : PubMed/NCBI
29.	Aronsson DE and Muhr C: Quantification of sensitivity of endothelial cell markers for the astrocytoma and oligodendroglioma tumors. Anticancer Res. 22:343–346. 2002.PubMed/NCBI
30.	Shivakumar S, Prabhakar BT, Jayashree K, Rajan MG and Salimath BP: Evaluation of serum vascular endothelial growth factor (VEGF) and microvessel density (MVD) as prognostic indicators in carcinoma breast. J Cancer Res Clin Oncol. 135:627–636. 2009. View Article : Google Scholar : PubMed/NCBI
31.	Tohama Y, Gratas C, van Meir EG, et al: Necrogenesis and Fas/APO-1 (CD95) expression in primary (de novo) and secondary glioblastomas. J Neuropathol Exp Neurol. 57:239–245. 1998.PubMed/NCBI
32.	Homma T, Fukushima T, Vaccarella S, et al: Correlation among pathology, genotype and patient outcomes in glioblastoma. J Neuropathol Exp Neurol. 65:846–854. 2006. View Article : Google Scholar : PubMed/NCBI
33.	Netto GC, Bleil CB, Hilbig A and Coutinho LM: Immunohistochemical evaluation of the microvascular density through the expression of TGF-β (CD 105/endoglin) and CD 34 receptors and expression of the vascular endothelial growth factor (VEGF) in oligodendrogliomas. Neuropathology. 28:17–23. 2008.PubMed/NCBI
34.	Vaquero J, Zurita M, Coca S, Oya S and Morales C: Prognostic significance of clinical and angiogenesis-related factors in low-grade oligodendrogliomas. Surg Neurol. 54:229–234. 2000. View Article : Google Scholar : PubMed/NCBI
35.	Ohgaki H and Kleihues P: Population-based studies on incidence, survival rates and genetic alterations in astrocytic and oligodendroglial gliomas. J Neuropathol Exp Neurol. 64:479–489. 2005.PubMed/NCBI
36.	Ohgaki H and Kleihues P: Genetic pathways to primary and secondary glioblastoma. Am J Pathol. 170:1445–1453. 2007. View Article : Google Scholar : PubMed/NCBI
37.	Folkerth RD: Descriptive analysis and quantification of angiogenesis in human brain tumors. J Neurooncol. 50:165–172. 2000. View Article : Google Scholar : PubMed/NCBI
38.	Traweek ST, Kandalaft PL, Metha P and Battifora H: The human hematopoietic progenitor cell antigen (CD34) in vascular neoplasia. Am J Clin Pathol. 96:25–31. 1991.PubMed/NCBI