Carboxyl-terminus of the amyloid protein precursor and ERβ are required for estrogenic effect in activating mitogen-activated protein kinase
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- Published online on: May 1, 2004 https://doi.org/10.3892/ijmm.13.5.691
- Pages: 691-696
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Abstract
Estrogen influences the processing of the amyloid β precursor protein (APP) in the pathogenesis of Alzheimer's disease, and this effect is mediated by estrogen receptors (ERs) in activating mitogen-activated protein kinase (MAPK)-signaling pathway. To test whether the estrogenic effect on both carboxyl-terminal amino acid fragment (C-terminal) of APP (APP-C105)- and ERβ-mediated MAPK activation in in vitro, two hybrid genes containing each human ERβ and APP-C105 gene fused to the neuron-specific enolase (NSE) promoter were constructed and were transfected to the neuronal SK-N-MC cells. Western blot shows that the activation of JNK-signaling pathway, but not p38 and ERK, is dependent on ERβ through estrogen treatment and APP-C105 is also mediated through estrogen in activating MAPK-signaling pathway. The results suggest that ERβ and APP-C105 derived from APP are necessary for estrogenic effect in activating MAPK-signaling pathway.
