Enhanced radiation-induced apoptosis of cancer cell lines after treatment with resveratrol

  • Authors:
    • Sarah Baatout
    • Hanane Derradji
    • Paul Jacquet
    • Daniella Ooms
    • Arlette Michaux
    • Max Mergeay
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  • Published online on: June 1, 2004     https://doi.org/10.3892/ijmm.13.6.895
  • Pages: 895-902
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Abstract

A search for new agents that can sensitise cancer cells to ionising radiation is of continual interest and mainly due to the use of radiation in cancer therapy. Resveratrol, a powerful antioxidant has been shown to inhibit carcinogenesis in animal models. The purpose of this study was to examine whether resveratrol can sensitise cancer cells to X-irradiation. The human cancer cell lines examined were HELA (cervix carcinoma), K-562 (chronic myeloid leukemia) and IM-9 (multiple myeloma). The assays that were performed following X-irradiation (doses from 0 to 8 Gy) and/or incubation in the presence of resveratrol (concentrations ranging from 0 to 200 µM), were the following: trypan blue exclusion test to determine cell viability, cell morphology after May-Grunwald Giemsa staining, DNA profile analysis by flow cytometry to assess cell cycle distribution and the presence of the sub-G1 peak. The cell lines showed different radiation sensitivity (IM-9, high radiation sensitivity, K-562, intermediate radiation sensitivity and HELA, low radiation sensitivity) as seen by the X-irradiation dose related inhibition of cell growth and induction of apoptosis. The addition of resveratrol alone to the cell cultures induced apoptosis and inhibited cell growth from 50 (IM-9), 100 (EOL-1) or 200 µM (HELA) resveratrol concentrations. Concomitant treatment of the cells with either resveratrol and X-irradiation induced a synergical effect at the highest dose of 200 µM. These results show that resveratrol can act as a potential radiation sensitiser at high concentrations. Further studies need to address the toxicity of resveratrol on normal cells.

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June 2004
Volume 13 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Baatout S, Derradji H, Jacquet P, Ooms D, Michaux A and Mergeay M: Enhanced radiation-induced apoptosis of cancer cell lines after treatment with resveratrol. Int J Mol Med 13: 895-902, 2004.
APA
Baatout, S., Derradji, H., Jacquet, P., Ooms, D., Michaux, A., & Mergeay, M. (2004). Enhanced radiation-induced apoptosis of cancer cell lines after treatment with resveratrol. International Journal of Molecular Medicine, 13, 895-902. https://doi.org/10.3892/ijmm.13.6.895
MLA
Baatout, S., Derradji, H., Jacquet, P., Ooms, D., Michaux, A., Mergeay, M."Enhanced radiation-induced apoptosis of cancer cell lines after treatment with resveratrol". International Journal of Molecular Medicine 13.6 (2004): 895-902.
Chicago
Baatout, S., Derradji, H., Jacquet, P., Ooms, D., Michaux, A., Mergeay, M."Enhanced radiation-induced apoptosis of cancer cell lines after treatment with resveratrol". International Journal of Molecular Medicine 13, no. 6 (2004): 895-902. https://doi.org/10.3892/ijmm.13.6.895