Cholestasis in a rat model of graft-versus-host disease is accompanied by alteration of the expression and distribution of tight-junction-associated proteins

Sasatomi,Kurumi; Sakisaka,Shotaro; Kawaguchi,Takumi; Hanada,Shinichiro; Taniguchi,Eitaro; Koga,Hironori; Harada,Masaru; Sata,Michio

doi:10.3892/ijmm.15.3.431

March 2005 Volume 15 Issue 3

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March 2005 Volume 15 Issue 3

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Article

Cholestasis in a rat model of graft-versus-host disease is accompanied by alteration of the expression and distribution of tight-junction-associated proteins

Authors:
- Kurumi Sasatomi
- Shotaro Sakisaka
- Takumi Kawaguchi
- Shinichiro Hanada
- Eitaro Taniguchi
- Hironori Koga
- Masaru Harada
- Michio Sata
View Affiliations / Copyright

Affiliations: Pittsburgh, PA 15217, USA. sasatomik@hotmail.com
Pages: 431-436
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Published online on: March 1, 2005

https://doi.org/10.3892/ijmm.15.3.431
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Abstract

Intrahepatic cholestasis has been recognized as one of the characteristic features of the hepatic manifestations in graft-versus-host disease (GVHD). Tight junctions (TJs) play crucial roles in bile formation and alterations of TJs in hepatocytes and/or biliary epithelial cells (BECs) that cause intrahepatic cholestasis. To assess the changes of TJs in hepatocytes and BECs in a rat model of GVHD, we examined the localization of TJ-associated proteins, 7H6 and zonula occludens (ZO)-1. GVHD was induced by injecting spleen cells of parental strain rats (Brown Norway) into non-irradiated (Brown Norway x Lewis) F1 hybrid rats. Untreated F1 hybrid rats served as controls. Double-labeled immunofluorescent staining for 7H6 and ZO-1 was performed in liver sections. In control rats, immunostaining for 7H6 and ZO-1 colocalized to the apical site of BECs and was continuous along the bile canaliculi. In GVHD, 7H6 expression was decreased in BECs and was discontinuous along the bile canaliculi. On the other hand, the intensity of ZO-1 staining in hepatocytes increased and did not change in BECs compared with that of control rats. Changes in TJ-associated proteins of both hepatocytes and BECs may reflect the immunopathogenesis of GVHD-associated intrahepatic cholestasis.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Cholestasis in a rat model of graft-versus-host disease is accompanied by alteration of the expression and distribution of tight-junction-associated proteins

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