Oxidative stress-induced poly(ADP-ribosyl)ation in chick limb bud-derived chondrocytes

  • Authors:
    • Róza Zákány
    • Edina Bakondi
    • Tamás Juhász
    • Csaba Matta
    • Zsolt Szíjgyártó
    • Katalin Erdélyi
    • éva Szabó
    • László Módis
    • László Virág
    • Pál Gergely
  • View Affiliations

  • Published online on: April 1, 2007     https://doi.org/10.3892/ijmm.19.4.597
  • Pages: 597-605
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Abstract

Oxidative stress has been implicated in the pathogenesis of various diseases affecting chondrogenesis or the function of articular cartilage. DNA damage caused by oxidative stress may trigger the activation of the nuclear enzyme, poly(ADP-ribose) polymerase-1 (PARP-1) which may contribute to tissue injury. We aimed at investigating the effects of peroxynitrite (100-600 µM) and hydrogen peroxide (0.1-4 mM) on PARP activation and extracellular matrix production of high density micromass cultures (HDC) prepared from chick limb bud mesenchymal cells. We found that both oxidative species strongly inhibited matrix formation of HDCs treated on day 2 but not on day 5. The PARP inhibitor 3-aminobenzamide (3-AB) stimulated matrix production in non-stressed cells and prevented suppressed matrix production in oxidatively stressed cells. Both hydrogen peroxide and peroxynitrite induced PARP activation and poly(ADP-ribose) accumulation. Decreased proliferation, viability and NAD+ content were not or only slightly improved by 3-AB, indicating that 3-AB directly affects matrix formation. In conclusion, oxidative stress stimulates poly(ADP-ribose) metabolism and inhibits extracellular matrix production of HDCs in a PARP-dependent manner. Our findings may have implications for potential therapeutic approaches aimed at restoring the matrix production capacity of chondrogenic cells.

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April 2007
Volume 19 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zákány R, Bakondi E, Juhász T, Matta C, Szíjgyártó Z, Erdélyi K, Szabó é, Módis L, Virág L, Gergely P, Gergely P, et al: Oxidative stress-induced poly(ADP-ribosyl)ation in chick limb bud-derived chondrocytes. Int J Mol Med 19: 597-605, 2007.
APA
Zákány, R., Bakondi, E., Juhász, T., Matta, C., Szíjgyártó, Z., Erdélyi, K. ... Gergely, P. (2007). Oxidative stress-induced poly(ADP-ribosyl)ation in chick limb bud-derived chondrocytes. International Journal of Molecular Medicine, 19, 597-605. https://doi.org/10.3892/ijmm.19.4.597
MLA
Zákány, R., Bakondi, E., Juhász, T., Matta, C., Szíjgyártó, Z., Erdélyi, K., Szabó, é., Módis, L., Virág, L., Gergely, P."Oxidative stress-induced poly(ADP-ribosyl)ation in chick limb bud-derived chondrocytes". International Journal of Molecular Medicine 19.4 (2007): 597-605.
Chicago
Zákány, R., Bakondi, E., Juhász, T., Matta, C., Szíjgyártó, Z., Erdélyi, K., Szabó, é., Módis, L., Virág, L., Gergely, P."Oxidative stress-induced poly(ADP-ribosyl)ation in chick limb bud-derived chondrocytes". International Journal of Molecular Medicine 19, no. 4 (2007): 597-605. https://doi.org/10.3892/ijmm.19.4.597