Alternative splicing of apoptosis-related genes in imatinib-treated K562 cells identified by exon array analysis

  • Authors:
    • Jing Liu
    • Yun Xiao
    • Huo-Mei Xiong
    • Jing Li
    • Bo Huang
    • Hai-Bin Zhang
    • Dan-Qin Feng
    • Xi-Min Chen
    • Xiao-Zhong Wang
  • View Affiliations

  • Published online on: December 29, 2011     https://doi.org/10.3892/ijmm.2011.872
  • Pages: 690-698
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Abstract

Imatinib is the therapeutic standard for newly diagnosed patients with chronic myeloid leukemia (CML). In these patients, imatinib has been shown to induce an apoptotic response specifically in cells expressing the oncogenic fusion protein BCR-ABL. Previous studies in our lab revealed that imatinib-induced apoptosis in K562 cells involves a shift in production of Bcl-x splice isoforms towards the pro-apoptotic Bcl-xs splice variant. Here, we report the findings from our subsequent study to identify other apoptosis-related genes that are differentially spliced in response to imatinib treatment. Gene expression profiling of imatinib-treated K562 cells was performed by the Affymetrix GeneChip® Human Exon 1.0 ST array, and differences in exon-level expression and alternative splicing were analyzed using the easyExon software. Detailed analysis by reverse transcription-PCR (RT-PCR) and sequencing of key genes confirmed the experimental results of the exon array. Our results suggest that imatinib treatment of K562 cells causes a transcriptional shift towards alternative splicing in a large number of apoptotic genes. The present study provides insight into the molecular character of apoptotic leukemia cells and may help to improve the mechanism of imatinib therapy in patients with CML.
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April 2012
Volume 29 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Liu J, Xiao Y, Xiong H, Li J, Huang B, Zhang H, Feng D, Chen X and Wang X: Alternative splicing of apoptosis-related genes in imatinib-treated K562 cells identified by exon array analysis. Int J Mol Med 29: 690-698, 2012.
APA
Liu, J., Xiao, Y., Xiong, H., Li, J., Huang, B., Zhang, H. ... Wang, X. (2012). Alternative splicing of apoptosis-related genes in imatinib-treated K562 cells identified by exon array analysis. International Journal of Molecular Medicine, 29, 690-698. https://doi.org/10.3892/ijmm.2011.872
MLA
Liu, J., Xiao, Y., Xiong, H., Li, J., Huang, B., Zhang, H., Feng, D., Chen, X., Wang, X."Alternative splicing of apoptosis-related genes in imatinib-treated K562 cells identified by exon array analysis". International Journal of Molecular Medicine 29.4 (2012): 690-698.
Chicago
Liu, J., Xiao, Y., Xiong, H., Li, J., Huang, B., Zhang, H., Feng, D., Chen, X., Wang, X."Alternative splicing of apoptosis-related genes in imatinib-treated K562 cells identified by exon array analysis". International Journal of Molecular Medicine 29, no. 4 (2012): 690-698. https://doi.org/10.3892/ijmm.2011.872