Identification of Pold2 as a novel interaction partner of protein inhibitor of activated STAT2

Zheng,Ying; Sheng,Shudong; Wang,Haiyan; Jia,Xiaoqing; Hu,Yanqiu; Qian,Yayun; Zhu,Yongze; Wang,Jianjun

doi:10.3892/ijmm.2012.1065

Identification of Pold2 as a novel interaction partner of protein inhibitor of activated STAT2

Authors:
- Ying Zheng
- Shudong Sheng
- Haiyan Wang
- Xiaoqing Jia
- Yanqiu Hu
- Yayun Qian
- Yongze Zhu
- Jianjun Wang
View Affiliations

Affiliations: Department of Histology and Embryology, Medical College, Yangzhou University, Yangzhou 225001, P.R. China, Department of Medicine, Yangzhou Vocational College of Environment and Resources, Yangzhou 225001, P.R. China, Center of Reproductive Medicine, Clinical Medical College, Yangzhou University, Yangzhou 225001, P.R. China
Published online on: July 16, 2012 https://doi.org/10.3892/ijmm.2012.1065
Pages: 884-888

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Pold2 is a subunit of the DNA polymerase δ complex, encoding a protein involved in DNA replication and repair. In this study, using a yeast two-hybrid screening technique and the common cDNA fragment of the mouse PIAS2 as a bait, Pold2 was found to interact with PIAS2. A direct interaction between Pold2 and PIAS2 was confirmed by direct yeast two-hybrid. In vivo evidence of Pold2 association with PIAS2 was obtained by co-immunoprecipitation using HEK-293 cells. Subcellular localization studies demonstrated that Pold2 and PIAS2 were partially co-localized in mammalian cells. Collectively, our results suggest that Pold2 interacts under physiological conditions with PIAS2.

View Figures

View References

1.	U HubscherM GiovanniS SpadariEukaryotic DNA polymerasesAnnu Rev Biochem71133163200210.1146/annurev.biochem.71.090501.150041
2.	MD SuttonGC WalkerManaging DNA polymerases: coordinating DNA replication, DNA repair, and DNA recombinationProc Natl Acad Sci USA9883428349200110.1073/pnas.11103699811459973
3.	SP BellA DuttaDNA replication in eukaryotic cellsAnnu Rev Biochem71333374200210.1146/annurev.biochem.71.110601.13542512045100
4.	MY LeeCK TanKM DowneyAG SoFurther studies on calf thymus DNA polymerase δ purified to homogeneity by a new procedureBiochemistry23190619131984
5.	SA MacNeillS MorenoN ReynoldsP NursePA FantesThe fission yeast Cdcl protein, a homologue of the small subunit of DNA polymerase 8, binds to Pol3 and Cdc27EMBO J154613462819968887553
6.	YI PavlovPV ShcherbakovaIB RogozinRoles of DNA polymerases in replication, repair, and recombination in eukaryotesInt Rev Cytol25541132200610.1016/S0074-7696(06)55002-817178465
7.	R MossiU HubscherClamping down on clamps and clamp loaders-the eukaryotic replication factor CEur J Biochem25420921619989660172
8.	Z KelmanPCNA: structure, functions and interactionsOncogene14629640199710.1038/sj.onc.12008869038370
9.	B XieN MazloumL LiuA RahmehH LiMY LeeReconstitution and characterization of the human DNA polymerase delta four-subunit holoenzymeBiochemistry411313313142200210.1021/bi026270712403614
10.	H LiB XieY ZhouA RahmehS TrusaS ZhangY GaoEY LeeMY LeeFunctional roles of p12, the fourth subunit of human DNA polymerase δJ Biol Chem2811474814755200616510448
11.	H LiB XieA RahmehY ZhouMY LeeDirect interaction of p21 with p50, the small subunit of human DNA polymerase deltaCell Cycle5428436200610.4161/cc.5.4.242516479163
12.	H HeCK TanKM DowneyAG SoA tumor necrosis factor α- and interleukin 6-inducible protein that interacts with the small subunit of DNA polymerase δ and proliferating cell nuclear antigenProc Natl Acad Sci USA9811979119842001
13.	L LiuEM Rodriguez-BelmonteN MazloumB XieMY LeeIdentification of a novel protein, PDIP38, that interacts with the p50 subunit of DNA polymerase δ and proliferating cell nuclear antigenJ Biol Chem2781004110047200312522211
14.	AM SzekelyYH ChenC ZhangJ OshimaSM WeissmanWerner protein recruits DNA polymerase δ to the nucleolusProc Natl Acad Sci USA971136511370200011027336
15.	B LiuJ LiaoX RaoSA KushnerCD ChungDD ChangK ShuaiInhibition of Stat1-mediated gene activation by PIAS1Proc Natl Acad Sci USA951062610631199810.1073/pnas.95.18.106269724754
16.	N KotajaS AittomäkiO SilvennoinenJJ PalvimoOA JänneARIP3 (androgen receptor-interacting protein 3) and other PIAS (protein inhibitor of activated STAT) proteins differ in their ability to modulate steroid receptor-dependent transcriptional activationMol Endocrinol1419862000200010.1210/mend.14.12.0569
17.	CD ChungJ LiaoB LiuX RaoP JayP BertaK ShuaiSpecific inhibition of Stat3 signal transduction by PIAS3Science27818031805199710.1126/science.278.5344.18039388184
18.	C ZhangX YuanL YueJ FuL LuoZ YinPIASy interacts with p73alpha and regulates cell cycle in HEK293 cellsCell Immunol263235240201010.1016/j.cellimm.2010.04.00520471636
19.	AD SharrocksPIAS proteins and transcriptional regulation-more than just SUMO E3 ligases?Genes Dev20754758200610.1101/gad.142100616600908
20.	K ShuaiRegulation of cytokine signaling pathways by PIAS proteinsCell Res16196202200610.1038/sj.cr.731002716474434
21.	T AroraB LiuH HeJ KimTL MurphyKM MurphyRL ModlinK ShuaiPIASx is a transcriptional co-repressor of signal transducer and activator of transcriptionJ Biol Chem2782132721330200310.1074/jbc.C30011920012716907
22.	Y PengJ LeeC ZhuZ SunA novel role for protein inhibitor of activated STAT (PIAS) proteins in modulating the activity of Zimp7, a novel PIAS-like protein, in androgen receptor-mediated transcriptionJ Biol Chem2851146511475201010.1074/jbc.M109.07932720159969
23.	M GrossB LiuJ TanFS FrenchM CareyK ShuaiDistinct effects of PIAS proteins on androgen-mediated gene activation in prostate cancer cellsOncogene2038803887200110.1038/sj.onc.120448911439351
24.	K TakahashiT TairaT NikiC SeinoSM Iguchi-ArigaH ArigDJ-1 positively regulates the androgen receptor by impairing the binding of PIASxα to the receptorJ Biol Chem2763755637563200111477070
25.	R HindgesU HübscherCloning, chromosomal localization, and interspecies interaction of mouse DNA polymerase delta small subunit (PolD2)Genomics444551199710.1006/geno.1997.48389286699
26.	AG BaranovskiyND BabayevaVG ListonIB RogozinEV KooninYI PavlovDG VassylyevTH TahirovX-ray structure of the complex of regulatory subunits of human DNA polymerase deltaCell Cycle730263036200810.4161/cc.7.19.672018818516
27.	J HarperG AdamiN WeiK KeyomarsiS ElledgeThe p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinasesCell75805816199310.1016/0092-8674(93)90499-G8242751
28.	A NodaY NingS VenableO Pereira-SmithJ SmithCloning of senescent cell-derived inhibitors of DNA synthesis using an expression screenExp Cell Res2119098199410.1006/excr.1994.10638125163
29.	J ChenPK JacksonMW KirschnerA DuttaSeparate domains of p21 involved in the inhibition of Cdk kinase and PCNANature374386388199510.1038/374386a07885482
30.	JM GulbisZ KelmanJ HurwitzM O’DonnellJ KuriyanStructure of the C-terminal region of p21(WAF1/CIP1) complexed with human PCNACell87297306199610.1016/S0092-8674(00)81347-18861913
31.	S AdhikaryM EilersTranscriptional regulation and transformation by Myc proteinsNat Rev Mol Cell Biol6635645200510.1038/nrm170316064138
32.	S HeroldM WanzelV BeugerC FrohmeD BeulT HillukkalaJ SyvaojaHP SaluzF HaenelM EilersNegative regulation of the mammalian UV response by Myc through association with Miz-1Mol Cell10509521200210.1016/S1097-2765(02)00633-012408820
33.	A WeberJ MarquardtD ElziN ForsterS StarkeA GlaumD YamadaPA DefossezJ DelrowRN EisenmanH ChristiansenM EilersZbtb4 represses transcription of P21CIP1 and controls the cellular response to p53 activationEMBO J2715631574200810.1038/emboj.2008.8518451802