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Gastrodin inhibits cell proliferation in vascular smooth muscle cells and attenuates neointima formation in vivo

  • Authors:
    • Lihua Zhu
    • Hongjing Guan
    • Changping Cui
    • Song Tian
    • Da Yang
    • Xinan Wang
    • Shuming Zhang
    • Lang Wang
    • Hong Jiang

  • View Affiliations

  • Published online on: August 20, 2012     https://doi.org/10.3892/ijmm.2012.1100
  • Pages: 1034-1040

  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Vascular smooth muscle cell (VSMC) proliferation plays a critical role in the development of vascular diseases. In the present study, we tested the efficacy and the mechanisms of action of gastrodin, a bioactive component of the Chinese herb Gastrodia elata Bl, in relation to platelet-derived growth factor-BB (PDGF-BB)-dependent cell proliferation and neointima formation after acute vascular injury. Cell experiments were performed with VSMCs isolated from rat aortas. WST and BrdU incorporation assays were used to evaluate VSMC proliferation. Eight-week-old C57BL/6 mice were used for the animal experiments. Gastrodin (150 mg/kg/day) was administered in the animal chow for 14 days, and the mice were subjected to wire injury of the left carotid artery. Our data demonstrated that gastrodin attenuated the VSMC proliferation induced by PDGF-BB, as assessed by WST assay and BrdU incorporation. Gastrodin influenced the S-phase entry of VSMCs and stabilised p27Kip1 expression. In addition, pre-incubation with sinomenine prior to PDGF-BB stimulation led to increased smooth muscle-specific gene expression, thereby inhibiting VSMC dedifferentiation. Gastrodin treatment also reduced the intimal area and the number of PCNA-positive cells. Furthermore, PDGF-BB-induced phosphorylation of ERK1/2, p38 MAPK, Akt and GSK3β was suppressed by gastrodin. Our results suggest that gastrodin can inhibit VSMC proliferation and attenuate neointimal hyperplasia in response to vascular injury. Furthermore, the ERK1/2, p38 MAPK and Akt/GSK3β signalling pathways were found to be involved in the effects of gastrodin.
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November 2012
Volume 30 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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  • Journal Metrics
  • Impact Factor: 5.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 10.7
  • CiteScore Rank: Q1: #35/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.994
  • SCImago Journal Rank (SJR): 0.922
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Spandidos Publications style
Zhu L, Guan H, Cui C, Tian S, Yang D, Wang X, Zhang S, Wang L and Jiang H: Gastrodin inhibits cell proliferation in vascular smooth muscle cells and attenuates neointima formation in vivo. Int J Mol Med 30: 1034-1040, 2012
APA
Zhu, L., Guan, H., Cui, C., Tian, S., Yang, D., Wang, X. ... Jiang, H. (2012). Gastrodin inhibits cell proliferation in vascular smooth muscle cells and attenuates neointima formation in vivo. International Journal of Molecular Medicine, 30, 1034-1040. https://doi.org/10.3892/ijmm.2012.1100
MLA
Zhu, L., Guan, H., Cui, C., Tian, S., Yang, D., Wang, X., Zhang, S., Wang, L., Jiang, H."Gastrodin inhibits cell proliferation in vascular smooth muscle cells and attenuates neointima formation in vivo". International Journal of Molecular Medicine 30.5 (2012): 1034-1040.
Chicago
Zhu, L., Guan, H., Cui, C., Tian, S., Yang, D., Wang, X., Zhang, S., Wang, L., Jiang, H."Gastrodin inhibits cell proliferation in vascular smooth muscle cells and attenuates neointima formation in vivo". International Journal of Molecular Medicine 30, no. 5 (2012): 1034-1040. https://doi.org/10.3892/ijmm.2012.1100