Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes

An,In-Sook; An,Sungkwan; Choe,Tae-Βoo; Kang,Sang-Μo; Lee,Jae  Ho; Park,In-Chul; Jin,Young-Woo; Lee,Su-Jae; Bae,Seunghee

doi:10.3892/ijmm.2012.1157

Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes

Authors:
- In-Sook An
- Sungkwan An
- Tae-Βoo Choe
- Sang-Μo Kang
- Jae Ho Lee
- In-Chul Park
- Young-Woo Jin
- Su-Jae Lee
- Seunghee Bae
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Affiliations: Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea, Department of Microbial Engineering, Konkuk University, Seoul 143-701, Republic of Korea, Laboratory of Molecular Oncology, Cheil General Hospital and Women's Healthcare Center, Kwandong University, College of Medicine, Seoul 100-380, Republic of Korea, Laboratory of Functional Genomics, Korea Institute of Radiological and Medical Sciences, Seoul 139-706, Republic of Korea, Division of Radiation Effect Research, Radiation Health Research Institute of KHNP, Seoul 132-703, Republic of Korea, Department of Chemistry, Hanyang University, Seoul 133-791, Republic of Korea, Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea
Published online on: October 15, 2012 https://doi.org/10.3892/ijmm.2012.1157
Pages: 1349-1356

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Abstract

This study aimed to evaluate the protective effects of Centella asiatica (C. asiatica) against ultraviolet B (UVB) damage in human keratinocytes using microRNA (miRNA) expression profiling analysis. Titrated extract of C. asiatica (TECA) demonstrated low cytotoxicity in normal human HaCaT keratinocytes only at low doses (<5 µg/ml). UVB (50 mJ/cm2) irradiation significantly decreased cell viability, and TECA treatment decreased the UVB toxicity. By using miRNA microarrays, we determined that 72 miRNAs had an altered expression following TECA treatment in UVB-irradiated keratinocytes (46 upregulated and 26 downregulated). Using an miRNA target gene prediction tool and Gene Ontology (GO) analysis, we determined that miRNAs with altered expression were functionally related with the inhibition of apoptosis and cell proliferation. Overall, these results provide meaningful information to facilitate the understanding of TECA-mediated UVB protection in human keratinocytes.

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