Oridonin induces apoptosis and senescence by increasing hydrogen peroxide and glutathione depletion in colorectal cancer cells

  • Authors:
    • Feng-Hou Gao
    • Feng Liu
    • Wei Wei
    • Li-Bin Liu
    • Mang-Hua Xu
    • Zhu-Ying Guo
    • Wei Li
    • Bin Jiang
    • Ying-Li Wu
  • View Affiliations

  • Published online on: January 24, 2012     https://doi.org/10.3892/ijmm.2012.895
  • Pages: 649-655
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Abstract

We recently demonstrated that oridonin could induce apoptosis and senescence of colon cancer cells in vitro and in vivo. However, the underlying mechanism remains unknown. In this study, the involvement of reactive oxygen species in oridonin-induced cell death and senescence was investigated in colon adenocarcinoma-derived SW1116 cells. Oridonin increased intracellular hydrogen peroxide levels and reduced the glutathione content in a dose-dependent manner. N-acetylcysteine, a reactive oxygen species scavenger, not only blocked the oridonin-induced increase in hydrogen peroxide and glutathione depletion, but also blocked apoptosis and senescence induced by oridonin, as evidenced by the decrease in Annexin V and senescence-associated β-galactosidase- positive cells and the inhibition of oridonin-induced upregulation of p53 and p16 and downregulation of c-Myc. Moreover, exogenous catalase could inhibit the increase in hydrogen peroxide and apoptosis induced by oridonin, but not the glutathione depletion and senescence. Furthermore, thioredoxin reductase (TrxR) activity was reduced by oridonin in vitro and in cells, which may cause the increase in hydrogen peroxide. In conclusion, the increase in hydrogen peroxide and glutathione depletion account for oridonin-induced apoptosis and senescence in colorectal cancer cells, and TrxR inhibition is involved in this process. Given the importance of TrxR as a novel cancer target in colon cancer, oridonin would be a promising clinical candidate. The mechanism of oridonin-induced inhibition of TrxR warrants further investigation.
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April 2012
Volume 29 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Gao F, Liu F, Wei W, Liu L, Xu M, Guo Z, Li W, Jiang B and Wu Y: Oridonin induces apoptosis and senescence by increasing hydrogen peroxide and glutathione depletion in colorectal cancer cells. Int J Mol Med 29: 649-655, 2012.
APA
Gao, F., Liu, F., Wei, W., Liu, L., Xu, M., Guo, Z. ... Wu, Y. (2012). Oridonin induces apoptosis and senescence by increasing hydrogen peroxide and glutathione depletion in colorectal cancer cells. International Journal of Molecular Medicine, 29, 649-655. https://doi.org/10.3892/ijmm.2012.895
MLA
Gao, F., Liu, F., Wei, W., Liu, L., Xu, M., Guo, Z., Li, W., Jiang, B., Wu, Y."Oridonin induces apoptosis and senescence by increasing hydrogen peroxide and glutathione depletion in colorectal cancer cells". International Journal of Molecular Medicine 29.4 (2012): 649-655.
Chicago
Gao, F., Liu, F., Wei, W., Liu, L., Xu, M., Guo, Z., Li, W., Jiang, B., Wu, Y."Oridonin induces apoptosis and senescence by increasing hydrogen peroxide and glutathione depletion in colorectal cancer cells". International Journal of Molecular Medicine 29, no. 4 (2012): 649-655. https://doi.org/10.3892/ijmm.2012.895