Suppression of RND3 activity by AES downregulation promotes cancer cell proliferation and invasion

  • Authors:
    • Hongwei Xia
    • Mingxing Li
    • Liang Chen
    • Weibing Leng
    • Dandan Yuan
    • Xiaohui Pang
    • Liu Chen
    • Ronghui Li
    • Qiulin Tang
    • Feng Bi
  • View Affiliations

  • Published online on: March 27, 2013     https://doi.org/10.3892/ijmm.2013.1321
  • Pages: 1081-1086
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Abstract

Amino-terminal enhancer of split (AES) is a member of the Groucho/TLE family. Although it has no DNA-binding site, AES can regulate transcriptional activity by interacting with transcriptional factors. Emerging evidence indicates that AES may play an important role in tumor metastasis, but the molecular mechanism is still poorly understood. In this study, we found that knockdown of AES by RNA interference (RNAi) downregulated RND3 expression at the mRNA and protein levels in MDA-MB-231 and HepG2, two cancer cell lines. Furthermore, luciferase assays showed that overexpression of AES significantly enhanced RND3 promoter activity. Moreover, inhibition of AES both in MDA-MB-231 and HepG2 cells by RNAi significantly promoted cell proliferation, cell cycle progression and invasion, consistent with the effects of RNAi-mediated RND3 knockdown in these cells. For the first time, data are presented showing that alteration of the malignant behavior of cancer cells by AES is related to RND3 regulation, and these findings also provide new insights into the mechanism of AES action in regulating tumor malignancy.
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May 2013
Volume 31 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Xia H, Li M, Chen L, Leng W, Yuan D, Pang X, Chen L, Li R, Tang Q, Bi F, Bi F, et al: Suppression of RND3 activity by AES downregulation promotes cancer cell proliferation and invasion. Int J Mol Med 31: 1081-1086, 2013.
APA
Xia, H., Li, M., Chen, L., Leng, W., Yuan, D., Pang, X. ... Bi, F. (2013). Suppression of RND3 activity by AES downregulation promotes cancer cell proliferation and invasion. International Journal of Molecular Medicine, 31, 1081-1086. https://doi.org/10.3892/ijmm.2013.1321
MLA
Xia, H., Li, M., Chen, L., Leng, W., Yuan, D., Pang, X., Chen, L., Li, R., Tang, Q., Bi, F."Suppression of RND3 activity by AES downregulation promotes cancer cell proliferation and invasion". International Journal of Molecular Medicine 31.5 (2013): 1081-1086.
Chicago
Xia, H., Li, M., Chen, L., Leng, W., Yuan, D., Pang, X., Chen, L., Li, R., Tang, Q., Bi, F."Suppression of RND3 activity by AES downregulation promotes cancer cell proliferation and invasion". International Journal of Molecular Medicine 31, no. 5 (2013): 1081-1086. https://doi.org/10.3892/ijmm.2013.1321