Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
June 2013 Volume 31 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June 2013 Volume 31 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

p53 interferes with microtubule-stabilizing agent-induced apoptosis in prostate and colorectal cancer cells

  • Authors:
    • Ji Young Kim
    • Jin-Yong Chung
    • Seung Gee Lee
    • Yoon-Jae Kim
    • Ji-Eun Park
    • Jeanho Yun
    • Young Chul Park
    • Byeong Gee Kim
    • Young Hyun Yoo
    • Jong-Min Kim
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy and Cell Biology, Dong-A University, Busan 602-714, Republic of Korea, Mitochondria Hub Regulation Center, Dong-A University, Busan 602-714, Republic of Korea, Department of Biochemistry, Dong-A University, Busan 602-714, Republic of Korea, Department of Microbiology and Immunology, Pusan National University School of Medicine, Yangsan, Gyeongnam 626-870, Republic of Korea, Department of Biology, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea, Medical Research Science Center, Dong-A University, Busan 602-714, Republic of Korea
  • Pages: 1388-1394
    |
    Published online on: April 5, 2013
       https://doi.org/10.3892/ijmm.2013.1333
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Taxanes are microtubule-stabilizing agents that have anticancer activity against several types of human solid tumors. Although the primary mechanism of action of these drugs is well understood, the signaling pathways that confer resistance to these agents in certain types of cancer remain poorly understood. In particular, the association of p53 with the mechanism(s) of taxane-mediated cell death is still controversial. In this study, we showed that p53 has a profound inhibitory effect on docetaxel (Doc)-induced apoptosis in prostate and colorectal cancer cells and that caspases play a critical role in this process. Doc induced prostate cancer cell apoptosis at high levels in p53-null PC3 cells, at intermediate levels in p53-mutant DU145 cells and at low levels in p53 wild-type LNCaP cells. While transient overexpression of p53 in PC3 cells suppressed Doc-induced apoptosis, knockdown of p53 in LNCaP cells increased apoptosis. This finding was further confirmed using an isogenic pair of colorectal cancer cell lines, HCT-116 p53-/- and p53+/+, indicating that p53 inhibits induction of apoptosis by Doc. To our knowledge, this is the first report describing that chemical or genetic knockout of p53 enhances the susceptibility of both prostate and colorectal cancer cells to Doc-induced apoptosis. These results may suggest an approach to stratify patients for regimens involving Doc.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Rowinsky EK: The development and clinical utility of the taxane class of antimicrotubule chemotherapy agents. Annu Rev Med. 48:353–374. 1997. View Article : Google Scholar : PubMed/NCBI

2 

Diaz JF and Andreu JM: Assembly of purified GDP-tubulin into microtubules induced by taxol and taxotere: reversibility, ligand stoichiometry, and competition. Biochemistry. 32:2747–2755. 1993. View Article : Google Scholar : PubMed/NCBI

3 

Lavelle F, Bissery MC, Combeau C, Riou JF, Vrignaud P and André S: Preclinical evaluation of docetaxel (Taxotere). Semin Oncol. 22:3–16. 1995.

4 

Crown J and O’Leary M: The taxanes: an update. Lancet. 355:1176–1178. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Wang TH, Popp DM, Wang HS, et al: Microtubule dysfunction induced by paclitaxel initiates apoptosis through both c-Jun N-terminal kinase (JNK)-dependent and -independent pathways in ovarian cancer cells. J Biol Chem. 274:8208–8216. 1999. View Article : Google Scholar

6 

Blagosklonny MV, Schulte TW, Nguyen P, Mimnaugh EG, Trepel J and Neckers L: Taxol induction of p21WAF1 and p53 requires c-raf-1. Cancer Res. 55:4623–4626. 1995.PubMed/NCBI

7 

Haldar S, Chintapalli J and Croce CM: Taxol induces bcl-2 phosphorylation and death of prostate cancer cells. Cancer Res. 56:1253–1255. 1996.PubMed/NCBI

8 

Bacus SS, Gudkov AV, Lowe M, et al: Taxol-induced apoptosis depends on MAP kinase pathways (ERK and p38) and is independent of p53. Oncogene. 20:147–155. 2001. View Article : Google Scholar : PubMed/NCBI

9 

Morse DL, Gray H, Payne CM and Gillies RJ: Docetaxel induces cell death through mitotic catastrophe in human breast cancer cells. Mol Cancer Ther. 4:1495–1504. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Kim JY, Chung JY, Lee SG, et al: Nuclear interaction of Smac/DIABLO with Survivin at G2/M arrest prompts docetaxel-induced apoptosis in DU145 prostate cancer cells. Biochem Biophys Res Commun. 350:949–954. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Woods CM, Zhu J, McQueney PA, Bollag D and Lazarides E: Taxol-induced mitotic block triggers rapid onset of a p53-independent apoptotic pathway. Mol Med. 1:506–526. 1995.PubMed/NCBI

12 

Debernardis D, Siré EG, De Feudis P, et al: p53 status does not affect sensitivity of human ovarian cancer cell lines to paclitaxel. Cancer Res. 57:870–874. 1997.PubMed/NCBI

13 

Takahashi M, Kigawa J, Minagawa Y, et al: Sensitivity to paclitaxel is not related to p53-dependent apoptosis in ovarian cancer cells. Eur J Cancer. 36:1863–1868. 2000. View Article : Google Scholar : PubMed/NCBI

14 

Osaki S, Nakanishi Y, Takayama K, Pei XH, Ueno H and Hara N: Alteration of drug chemosensitivity caused by the adenovirus-mediated transfer of the wild-type p53 gene in human lung cancer cells. Cancer Gene Ther. 7:300–307. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Sezgin C, Karabulut B, Uslu R, et al: Potential predictive factors for response to weekly paclitaxel treatment in patients with metastatic breast cancer. J Chemother. 17:96–103. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Reinecke P, Kalinski T, Mahotka C, et al: Paclitaxel/Taxol sensitivity in human renal cell carcinoma is not determined by the p53 status. Cancer Lett. 222:165–171. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Wahl AF, Donaldson KL, Fairchild C, et al: Loss of normal p53 function confers sensitization to Taxol by increasing G2/M arrest and apoptosis. Nat Med. 2:72–79. 1996. View Article : Google Scholar : PubMed/NCBI

18 

Cassinelli G, Supino R, Perego P, et al: A role for loss of p53 function in sensitivity of ovarian carcinoma cells to taxanes. Int J Cancer. 92:738–747. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Vogelstein B: Cancer. A deadly inheritance. Nature. 348:681–682. 1990. View Article : Google Scholar : PubMed/NCBI

20 

Bates S and Vousden KH: p53 in signaling checkpoint arrest or apoptosis. Curr Opin Genet Dev. 6:12–18. 1996. View Article : Google Scholar : PubMed/NCBI

21 

Clarke AR, Purdie CA, Harrison DJ, et al: Thymocyte apoptosis induced by p53-dependent and independent pathways. Nature. 362:849–852. 1993. View Article : Google Scholar : PubMed/NCBI

22 

Lee JM and Bernstein A: p53 mutations increase resistance to ionizing radiation. Proc Natl Acad Sci USA. 90:5742–5746. 1993. View Article : Google Scholar : PubMed/NCBI

23 

Lowe SW, Schmitt EM, Smith SW, Osborne BA and Jacks T: p53 is required for radiation-induced apoptosis in mouse thymocytes. Nature. 362:847–849. 1993. View Article : Google Scholar : PubMed/NCBI

24 

Brown R, Clugston C, Burns P, et al: Increased accumulation of p53 protein in cisplatin-resistant ovarian cell lines. Int J Cancer. 55:678–684. 1993. View Article : Google Scholar : PubMed/NCBI

25 

Fan S, Smith ML, Rivet DJ II, et al: Disruption of p53 function sensitizes breast cancer MCF-7 cells to cisplatin and pentoxifylline. Cancer Res. 55:1649–1654. 1995.PubMed/NCBI

26 

Hawkins DS, Demers GW and Galloway DA: Inactivation of p53 enhances sensitivity to multiple chemotherapeutic agents. Cancer Res. 56:892–898. 1996.PubMed/NCBI

27 

van Bree C, Savonije JH, Franken NA, Haveman J and Bakker PJ: The effect of p53-function on the sensitivity to paclitaxel with or without hyperthermia in human colorectal carcinoma cells. Int J Oncol. 16:739–744. 2000.PubMed/NCBI

28 

Matsuhashi N, Saio M, Matsuo A, Sugiyama Y and Saji S: Apoptosis induced by 5-fluorouracil, cisplatin and paclitaxel are associated with p53 gene status in gastric cancer cell lines. Int J Oncol. 26:1563–1567. 2005.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Kim JY, Chung J, Lee SG, Kim Y, Park J, Yun J, Park YC, Kim BG, Yoo YH, Kim J, Kim J, et al: p53 interferes with microtubule-stabilizing agent-induced apoptosis in prostate and colorectal cancer cells. Int J Mol Med 31: 1388-1394, 2013.
APA
Kim, J.Y., Chung, J., Lee, S.G., Kim, Y., Park, J., Yun, J. ... Kim, J. (2013). p53 interferes with microtubule-stabilizing agent-induced apoptosis in prostate and colorectal cancer cells. International Journal of Molecular Medicine, 31, 1388-1394. https://doi.org/10.3892/ijmm.2013.1333
MLA
Kim, J. Y., Chung, J., Lee, S. G., Kim, Y., Park, J., Yun, J., Park, Y. C., Kim, B. G., Yoo, Y. H., Kim, J."p53 interferes with microtubule-stabilizing agent-induced apoptosis in prostate and colorectal cancer cells". International Journal of Molecular Medicine 31.6 (2013): 1388-1394.
Chicago
Kim, J. Y., Chung, J., Lee, S. G., Kim, Y., Park, J., Yun, J., Park, Y. C., Kim, B. G., Yoo, Y. H., Kim, J."p53 interferes with microtubule-stabilizing agent-induced apoptosis in prostate and colorectal cancer cells". International Journal of Molecular Medicine 31, no. 6 (2013): 1388-1394. https://doi.org/10.3892/ijmm.2013.1333
Copy and paste a formatted citation
x
Spandidos Publications style
Kim JY, Chung J, Lee SG, Kim Y, Park J, Yun J, Park YC, Kim BG, Yoo YH, Kim J, Kim J, et al: p53 interferes with microtubule-stabilizing agent-induced apoptosis in prostate and colorectal cancer cells. Int J Mol Med 31: 1388-1394, 2013.
APA
Kim, J.Y., Chung, J., Lee, S.G., Kim, Y., Park, J., Yun, J. ... Kim, J. (2013). p53 interferes with microtubule-stabilizing agent-induced apoptosis in prostate and colorectal cancer cells. International Journal of Molecular Medicine, 31, 1388-1394. https://doi.org/10.3892/ijmm.2013.1333
MLA
Kim, J. Y., Chung, J., Lee, S. G., Kim, Y., Park, J., Yun, J., Park, Y. C., Kim, B. G., Yoo, Y. H., Kim, J."p53 interferes with microtubule-stabilizing agent-induced apoptosis in prostate and colorectal cancer cells". International Journal of Molecular Medicine 31.6 (2013): 1388-1394.
Chicago
Kim, J. Y., Chung, J., Lee, S. G., Kim, Y., Park, J., Yun, J., Park, Y. C., Kim, B. G., Yoo, Y. H., Kim, J."p53 interferes with microtubule-stabilizing agent-induced apoptosis in prostate and colorectal cancer cells". International Journal of Molecular Medicine 31, no. 6 (2013): 1388-1394. https://doi.org/10.3892/ijmm.2013.1333
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team